A method for producing bletilla striata seedlings based on liquid medium

The technology of a liquid culture medium and a production method is applied in the field of Bletilla striata seedling production based on the liquid culture medium, and can solve the problems of large proliferation usage, increased cost, long time and the like, and achieves the effects of low cost, consistent emergence, and easy operation.

Active Publication Date: 2018-05-11
成都极谷基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with common hormones such as BA, TDZ is slightly more expensive. It is used in a large amount for proliferation and takes a long time, which increases the cost

Method used

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  • A method for producing bletilla striata seedlings based on liquid medium
  • A method for producing bletilla striata seedlings based on liquid medium
  • A method for producing bletilla striata seedlings based on liquid medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 immature bletilla striata culture

[0031] 1. Seed surface sterilization and germination

[0032] After 15 weeks of flowering and pollination, uncracked Bletilla striata capsules (including seeds) were surface-sterilized with 75% ethanol for 30 seconds on an ultra-clean workbench, and washed 1-2 times with sterile water. Carefully peel off the fruit with a scalpel and tweezers, carefully shake off the seeds into a sterile tissue culture bottle, add an appropriate amount of 5% sodium hypochlorite solution (1-2 drops of Tween 20) for disinfection for 20 minutes. Filter the seeds with a sterilized 50-mesh cell sieve; rinse and filter with sterile water, repeat 4-6 times, and wash away the residual disinfectant on the surface. The seeds filtered on the cell sieve were rinsed into the Erlenmeyer flask with liquid seed germination medium of MS+1.0mg / L TDZ+30g / L white granulated sugar, pH5.8. 25°C, 100rpm, dark culture for 15-20 days.

[0033] About 3 days late...

Embodiment 2

[0042] Example 2 Cultivation of nearly mature bletilla striata

[0043] 1. Seed surface sterilization and germination:

[0044] More than 20 weeks after flowering, the nearly mature Bletilla striata capsules (uncracked, containing seeds) are wrapped in gauze and washed with running water for 30 minutes; the surface is disinfected with 75% ethanol for 30 seconds on an ultra-clean workbench, and washed 1-2 times with sterile water . Carefully peel off the fruit with a scalpel and tweezers, carefully put the seeds into a sterile tissue culture bottle, add an appropriate amount of 5% sodium hypochlorite solution (add 2-3 drops of Tween) to disinfect for 20 minutes. Filter the seeds with a sterilized cell sieve (50 mesh); rinse and filter with sterile water, repeat 4-6 times, and wash away the residual disinfectant on the surface. The seeds filtered on the cell sieve were rinsed into the Erlenmeyer flask with MS+2.0mg / L TDZ+30g / L white granulated sugar, pH5.8 liquid seed germinat...

Embodiment 3

[0051] Embodiment 3 mature bletilla striata culture

[0052] 1. Seed surface sterilization and germination:

[0053] Mature Bletilla striata capsules (uncracked, containing seeds) were wrapped in gauze and rinsed with running water for 30 minutes; the surface was disinfected with 75% ethanol for 30 seconds on an ultra-clean workbench, and rinsed with sterile water 1-2 times. Carefully peel off the fruit with a scalpel and tweezers, carefully put the seeds into a sterile tissue culture bottle, add an appropriate amount of 5% sodium hypochlorite solution (add 2-3 drops of Tween) to disinfect for 20 minutes. Filter the seeds with a sterilized cell sieve (50 mesh); rinse and filter with sterile water, repeat 4-6 times, and wash away the residual disinfectant on the surface. The seeds filtered on the cell sieve were washed into the Erlenmeyer flask with MS+0.5mg / L TDZ+30g / L white granulated sugar, pH5.8 liquid seed germination liquid medium. 25° C., 100 rpm, and culture in the da...

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Abstract

The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for producing bletilla striata seedlings based on a liquid medium. The technical problem to be solved by the invention is to provide a new method for rapidly obtaining bletilla striata seedlings. The method comprises the following steps: a. sterilization; b. germination; c. growing into buds; d. rooting and culturing; e. hardening the grown seedlings and transplanting. The method is easy to operate and low in cost, and tens of thousands of seeds can be germinated in each bottle of liquid culture medium, and the seeds germinate neatly, emerge uniformly, and have low pollution rate. The present invention finally realizes the efficient and fast production of Bletilla striata tissue-cultured seedlings, effectively simplifies the operation procedure, reduces the production cost, and is convenient for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for producing bletilla striata seedlings based on a liquid medium. Background technique [0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae, and it is a national key protected wild plant. The flowers of Bletilla striata are large and colorful, and have high ornamental value. At the same time, Bletilla striata has massive pseudobulbs with thick flesh, which is one of the important Chinese medicinal materials. [0003] In the natural wild state, mature Bletilla striata seeds are powdery, very small, and mostly spindle-shaped under the microscope, about 1.2mm long and 0.2mm wide. The structure of its seeds is very simple, consisting of a transparent seed coat and a seed embryo. The seed coat is composed of a single layer of cells, the organelles and protoplasm have disappeared, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 邓科君郑雪莲张勇
Owner 成都极谷基因科技有限公司
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