Carbon dioxide biochemical analysis dry plate and preparation method thereof

Abstract

The invention relates to a carbon dioxide biochemical analysis dry plate and a preparation method thereof, and belongs to the technical field of blood-gas biochemical sensors. The dry plate is used for detecting the concentration of carbon dioxide in a body fluid, and comprises a supporting layer, an insulating layer, a salt bridge, electrodes, a reference layer, a carbonate ion selective membrane and a buffering layer. According to the preparation method, metal / metal salt thin slice electrodes are prepared, the reference layer is attached onto the electrodes, the ratio of sodium chloride to potassium chloride in the reference layer and the water content of the reference layer are constant, the carbonate ion selective membrane and the buffering layer are sequentially attached onto the solid reference layer, carbon dioxide selective electrodes are prepared, and hydroxyl vinyl resin is utilized as a bonding agent for the carbonate ion selective membrane. The prepared dry plate provided by the invention overcomes the defects that the conventional ion selective electrode is complex to operate, high in use cost and maintenance cost, slow in response, not easy to carry, and the like, and has the advantages of being simple to operate, high in response speed, small in size, simple in preparation process, low in production cost, and suitable for industrialized production.

Description

technical field [0001] The invention relates to the technical field of blood gas biochemical sensors, in particular to a carbon dioxide biochemical analysis dry sheet and a preparation method thereof. Background technique [0002] The carbon dioxide concentration in the blood is one of the important indicators reflecting the body's respiratory acid and alkalosis, which is helpful for the diagnosis and treatment of diseases caused by acid-base balance disorders, and is one of the indispensable inspection items in hospitals. [0003] In clinical laboratory medicine, there are four principles for the determination of carbon dioxide concentration, namely, based on ion-selective electrodes, based on ion-sensitive field effect tubes, based on the principle of gas-spectrum selective absorption, and based on the determination of gas-permeable skin characteristics. Among them, the test application based on the principle of ion selective electrode is the most widely used. [0004] Ch...

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Problems solved by technology

[0004] Chinese patent CN102156157A discloses an all-solid-state carbonate ion-selective electrode and its preparation method, but the ion-selective electrode in the patent needs to be paired with an external reference electrode when testing, and the method of use is complicated

Chinese patent CN103868965A discloses a CO 2 Microelectrode and its preparation method, the patent is to indirectly calculate the carbon dioxide content by testing the concentration of hydrogen ions in the liquid to be tested, the process is relatively complicated

[0005] The existing patents basically use screen printing technology to prepare electrodes, but the metal layer produced by screen printing is not pure enough and the surface is uneven, which will affect the stability of the potential and response time; in the existing solid-state ion selective electrodes, all use polychloride Ethylene is used as a polymer binder for ion-selective membranes (patents CN101852761A, CN104330449A, CN102636532A, CN101871912A, CN102558724A), polyvinyl chloride has high strength and chemical stability, and is cheap, but it must add plasticizers to ensure Ion-selective membrane response, as time goes by, the plasticizer and the carrier dissolved in it will leak out of the ion-selective membrane together, resulting in a poorer lower limit of the ion-selective membrane response and a shorter life

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