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Bionic tissue engineering scaffold containing inner channel network and oriented pore structure as well as preparation method and application of bionic tissue engineering scaffold

A technology of tissue engineering scaffold and pore structure, which is applied in medical science, prosthesis, surgery, etc., to achieve good penetration characteristics, facilitate transmission, and improve the effect of cell planting density and depth

Active Publication Date: 2016-02-10
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method does not form a hierarchically connected vascular network, and cannot fundamentally solve the problem of oxygen supply to the cells inside the scaffold.

Method used

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  • Bionic tissue engineering scaffold containing inner channel network and oriented pore structure as well as preparation method and application of bionic tissue engineering scaffold
  • Bionic tissue engineering scaffold containing inner channel network and oriented pore structure as well as preparation method and application of bionic tissue engineering scaffold
  • Bionic tissue engineering scaffold containing inner channel network and oriented pore structure as well as preparation method and application of bionic tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1. Bionic Structure Tissue Engineering Scaffold for Rat Myocardial Defect Repair

[0058] Gelatin (Gelatin, 15000-250000Da) was dissolved in PBS solution to prepare a gelatin solution with a mass fraction of 0.5%; sodium alginate was dissolved in PBS solution to prepare a sodium alginate solution with a mass fraction of 3.0%; The above two solutions are prepared into a uniform, bubble-free, and precipitation-free mixed solution. The mass ratio of gelatin to sodium alginate is 1:1; PLGA (PLA:PGA=75:25, molar ratio, viscosity 0.93dl / g , CHCl 3 / 25°C) was dissolved in chloroform to prepare a PLGA solution with a concentration of 2 mg / mL. Mix sucrose, maltose, and glucose uniformly in a mass ratio of 1:12:2, put them into the nozzle chamber of the air-pressure-based melt extrusion equipment, heat and melt at 130°C, and continuously extrude half Solidified sugar, according to the pre-designed structure and path to build a multi-branched soluble core (such as imag...

Embodiment 2

[0064] Example 2: Containing internal channel and directional structure bionic scaffold for construction of three-dimensional skeletal muscle tissue in vitro

[0065] Prepare 1wt% acetic acid solution. Rat tail type I collagen (SIGMA company, product number C7661) was dissolved in acetic acid solution to prepare a collagen solution with a mass fraction of 1.0%; chitosan was dissolved in an acetic acid solution to prepare a shell with a mass fraction of 1.0%. Polycan solution: Prepare the above two solutions into a uniform, bubble-free, and precipitate-free mixed solution, and the mass ratio of collagen to chitosan is 1:5. Dissolve PDLGA in chloroform to prepare a PDLGA solution with a concentration of 0.4 mg / mL. Preparation of PDMS molds containing channel structures, such as figure 2 As shown, gelatin was cast in it to construct a multi-branched gelatin network structure. After the inner core is formed, the gelatin network structure is soaked in PDLGA solution and sealed ...

Embodiment 3

[0068] Example 3. The tissue engineering scaffold with bionic structure is used for the repair of rat sciatic nerve defect

[0069] Prepare 2wt% acetic acid solution. Silk fibroin (silkfiber) is dissolved in the acetic acid solution, is prepared into the silk fibroin solution that mass fraction is 0.4%; Chitosan is dissolved in the acetic acid solution, is prepared into the chitosan solution that mass fraction is 2.0%; The above two solutions were prepared into a uniform, bubble-free, and precipitate-free mixed solution, and the mass ratio of silk fibroin to chitosan was 1:1. The alkali-soluble photosensitive polymer (copolymer of dimethyl-acrylamide, methacrylic acid and polyvinylpyrrolidone, the mass ratio of the three is 11:11:3), according to the pre-designed structure and path (such as image 3 As shown in (b), a multi-branched alkali-soluble network structure is constructed by stereolithography. Fix the network structure in the longitudinal direction of the directional...

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Abstract

The invention discloses a bionic tissue engineering scaffold containing an inner channel network and an oriented pore structure as well as a preparation method and application of the bionic tissue engineering scaffold. The bionic tissue engineering scaffold comprises a scaffold main body and a channel network, wherein the channel network is arranged inside the scaffold main body; the scaffold main body has the oriented micro-pore structure, and oriented big holes of the oriented micro-pore structure are mutually communicated by virtue of transverse small holes; the channel network has a hierarchical multi-branch structure, and the branches of the channel network are mutually communicated; and the scaffold main body is made from a degradable natural high polymer material. The bionic tissue engineering scaffold disclosed by the invention, which combines a 'core' manufacturing process with an 'oriented pore' directional crystallization and thermally induced phase separation process on the basis of thinking from 'presetting' to 're-dissolving', can break through the technical bottleneck of a complex micro-channel structure which is directly formed. By virtue of a rapid forming technology or a mold casting method, and in particular by virtue of the rapid forming technology, a core forming structure becomes flexible, so that a complex channel structure in any morphologies are formed; and the channel structure is good in passing-through characteristic.

Description

technical field [0001] The invention relates to a biomimetic tissue engineering scaffold containing an internal channel network and a directional pore structure, a preparation method and application thereof, and belongs to the technical field of tissue engineering and biomanufacturing. Background technique [0002] Tissue Engineering (Tissue Engineering) is a cutting-edge interdisciplinary science. Its basic principle is: by constructing a three-dimensional complex of cells and scaffolds in vitro, simulating the environment in vivo, and cultivating and training them to obtain tissues with repair or replacement functions. At present, tissue engineering has developed rapidly in the fields of skin, bone / cartilage, bladder, blood vessels, liver, nerves, etc., and has brought new hope to most medical problems faced by human beings, such as cardiovascular disease, Alzheimer's disease, Parkinson's disease, congenital genetic defects and other diseases and the treatment of various t...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/50A61L27/24A61L27/20A61L27/22A61L27/26A61L31/14A61L31/04
Inventor 张婷方永聪孙伟林峰张磊赵亚玲
Owner TSINGHUA UNIV
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