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Method for biological functionalization of silicon-based material surface

A biofunctionalized, silicon-based material technology, applied in the treatment of dyed organosilicon compounds, fibrous fillers, etc., can solve the problems of inactivation of biomolecules, high reaction specificity, and low coupling rate of biomolecules. Strong operability, wide applicability and high reproducibility

Inactive Publication Date: 2016-02-03
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Commonly used biomodification methods mainly include two types, one is to introduce carboxyl groups with succinic anhydride after aminosilane modification, and use succinimide to activate carboxyl groups to introduce biomolecules, but this method is only applicable to biomolecules containing amino groups, And the biomolecular coupling rate is low
Another method is to introduce certain chemical functional groups through specific silane coupling reagents, such as halosilane coupling agents, epoxy silane coupling agents, etc., to achieve biomolecular immobilization, but this method has high reaction specificity, Moreover, heating or metal catalysts are required during the reaction, which may cause the inactivation of biomolecules

Method used

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  • Method for biological functionalization of silicon-based material surface
  • Method for biological functionalization of silicon-based material surface
  • Method for biological functionalization of silicon-based material surface

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Embodiment approach

[0021] In another specific embodiment, the method includes the following steps:

[0022] (1) Surface modification of hydroxylated silicon-based materials with mercapto / aminosilane coupling agents;

[0023] (2) Divinyl sulfone is mixed with biomolecules and reacted at 0-40°C for 2-16 hours;

[0024] (3) Put the surface-modified silicon-based material in the mixed solution of step (2), keep it at 0-40° C. for 1-16 hours, take it out, and wash it with water and ethanol.

[0025] In any embodiment of the above-mentioned method for biofunctionalizing the surface of silicon-based materials of the present invention, in the reaction in which the silicon-based material reaction unit participates, the reaction temperature is 0-40°C, preferably 20-30°C; the reaction time is 1-16°C. hours, preferably 8-12 hours; in the reaction in which the biomolecular reaction unit participates, the reaction temperature is 0-40°C, preferably 20-30°C; the reaction time is 1-16 hours, preferably 8-12 hou...

Embodiment 1

[0033] Example 1: Preparation and performance testing of HCG functionalized optical fiber

[0034] Optical fiber (optical fiber) is a common silicon-based material, which is an ideal optical material due to the very low loss of light transmission in it. On the other hand, optical fiber has good biocompatibility, low price, and wide commercial sources, making it a widely used biosensing and medical detection element. In this embodiment, the biological molecule HCG is immobilized on the end face of the optical fiber to realize its biological function and be used for the detection of HCG antibody.

[0035] A fiber optic probe modified with an aminosilane coupling agent (DipandRead TMAminopropylsilane (APS) Biosensors, Fortebio, MenloPark, CA, USA) was immersed in an aqueous solution of divinyl sulfone (DVS) (10%, v / v, pH9.5, containing 10% acetone to aid dissolution), and activated at room temperature for 12 hours Then take it out, wash it with deionized water for 3 times, and ...

Embodiment 2

[0037] Example 2: Preparation and performance test of lactose-based functionalized glass sheet

[0038] Ultrasonic clean the glass slices twice with water and ethanol respectively, and place the cleaned glass slices in piranha lotion (sulfuric acid-hydrogen peroxide solution, concentrated sulfuric acid:hydrogen peroxide=3:1, v / v, note: piranha lotion is Organic matter is highly corrosive, please use it with care.) in 90°C for 30 minutes, ultrasonically cleaned with water for 3 times to achieve hydroxylation of the glass sheet. The hydroxylated glass slides were placed in 3-aminopropyltrimethoxysilane (APTES) solution (2%, v / v, the solvent was 95% ethanol, containing 5 drops of acetic acid) at room temperature for 24 hours, and the ethanol was ultrasonically cleaned for 3 The second time, vacuum crosslinking at 120°C for 4 hours to achieve amino modification of glass flakes. Place the amino-modified glass slide in a divinyl sulfone (DVS) solution (10%, v / v, pH9.5, containing 1...

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Abstract

The invention discloses a method for biological functionalization of a silicon-based material surface and a functionalized silicon-based material prepared by the method. The method is a method for connecting a hydroxylated silicon-based material subjected to surface modification through a sulfydryl / aminosilane coupling agent and biomolecules by divinyl sulphone. The method takes the commercialized sulfydryl / aminosilane coupling agent and the divinyl sulphone as coupling reagents, and is free from pretreatment, high in operability, high in reproducibility and wide in applicability; the divinyl sulphone can react with sulfydryl, azyl and hydroxyl, is suitable for the biomolecules and has broad spectrum property; related reactions are performed in an aqueous solution; the method is mild in condition and environment-friendly, and is a broad-spectrum method with large potential for biological functionalization of the silicon-based material surface.

Description

technical field [0001] The invention relates to a method and application of biological functionalization of the surface of silicon-based materials, in particular to a method and application of biologically active and inert functionalization of the surface of inorganic silicon materials based on natural biomolecules. technical background [0002] As a material with low price, many types, wide application, high stability and strong processability, silicon-based materials can be seen everywhere in our daily life and scientific research, such as single crystal silicon, glass, optical fiber, etc., are widely used in Aerospace, electrical and electronic, construction, transportation, energy, chemical, textile, food, light industry, medical, agricultural and other industries. On the other hand, silicon-based materials have good stability and biocompatibility under physiological conditions, making them widely used in biomedical fields such as cell culture, biochips, drug delivery, a...

Claims

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Application Information

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IPC IPC(8): C09C1/28C09C3/12
Inventor 程昉王汉奇孙世猷李明洋孙蔷陆安慧曲景平彭伟
Owner DALIAN UNIV OF TECH
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