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A bacitracin-fixed titanium alloy prosthesis for anti-inflammation, anti-infection and promotion of osteogenic differentiation and bone mineralization and its preparation method

A technology of osteogenic differentiation and titanium alloy, applied in the direction of chemical instruments and methods, prostheses, peptides, etc., to achieve the effect of inhibiting inflammatory reactions, mild reaction conditions, and appropriate setting of reaction parameters

Inactive Publication Date: 2018-04-17
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the titanium alloy prosthesis modified by bacitracin immobilization

Method used

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  • A bacitracin-fixed titanium alloy prosthesis for anti-inflammation, anti-infection and promotion of osteogenic differentiation and bone mineralization and its preparation method
  • A bacitracin-fixed titanium alloy prosthesis for anti-inflammation, anti-infection and promotion of osteogenic differentiation and bone mineralization and its preparation method
  • A bacitracin-fixed titanium alloy prosthesis for anti-inflammation, anti-infection and promotion of osteogenic differentiation and bone mineralization and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 Preparation of bacitracin-fixed titanium alloy prosthesis of the present invention (1)

[0045] 1. Introduction of functional functional group amino group on the surface of titanium sheet

[0046] A functional functional group amino group was introduced on the surface of titanium alloy by a two-step method. First, the titanium alloy Ti6AL4V (Shanghai Yantai Metal Materials Co., Ltd.) immersed in NaOH solution with a concentration of 5M, and reacted at 80°C for 12h. Then take out the titanium sheet and ultrasonically clean it three times with deionized water, soak it in deionized water after taking it out to remove the residual alkaline solution, and dry it. The titanium sheet was then immersed in 2 mg / ml dopamine Tris-HCl (pH=8.5) solution, reacted at 30° C. for 8 h, ultrasonically cleaned three times with deionized water, and dried.

[0047] 2. Covalent bond immobilization of bacitracin on the surface of titanium sheet

[0048] Put the titanium sheet ...

Embodiment 2

[0067] Embodiment 2 Preparation of bacitracin-fixed titanium alloy prosthesis of the present invention (two)

[0068] 1. Introduction of functional functional group amino group on the surface of titanium sheet

[0069] A functional functional group amino group was introduced on the surface of titanium alloy by a two-step method. Firstly, the titanium alloy disc was immersed in NaOH solution with a concentration of 10M, and reacted at 60°C for 10h. Then take out the titanium sheet and ultrasonically clean it three times with deionized water, soak it in deionized water after taking it out to remove the residual alkaline solution, and dry it. The titanium sheet was then immersed in a 5 mg / ml dopamine Tris-HCl (pH=9) solution, reacted at 25° C. for 12 hours, ultrasonically cleaned three times with deionized water, and dried.

[0070] 2. Covalent bond immobilization of bacitracin on the surface of titanium sheet

[0071] Put the titanium sheet introducing the functional function...

Embodiment 3

[0071] Put the titanium sheet introducing the functional functional group amino into the acetic acid solution of bacitracin (the concentration of bacitracin is 5 mg / ml, prepared with 5 μM acetic acid), pH=3, add 1-(3-dimethylaminopropyl)- 3-Ethylcarbodiimide (EDC) aqueous solution and N-hydroxysuccinimide (NHS) aqueous solution, so that the final concentration of EDC is 2.5 mg / mL, and the final concentration of NHS is 0.35 mg / mL, under inert gas nitrogen reflux The reaction was terminated after 6 hours of protection reaction, and the temperature during the reaction was 37°C. Ultrasonic cleaning with deionized water and vacuum drying. Embodiment 3 Preparation of bacitracin-fixed titanium alloy prosthesis of the present invention (three)

[0072] 1. Introduction of functional functional group amino group on the surface of titanium sheet

[0073] A functional functional group amino group was introduced on the surface of titanium alloy by a two-step method. Firstly, the titanium ...

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Abstract

The invention relates to a bacitracin-fixed titanium alloy prosthesis for anti-inflammation, anti-infection and promotion of osteogenic differentiation and bone mineralization and a preparation method thereof. The present invention immobilizes bacitracin on the titanium alloy by covalent grafting method, and the obtained bacitracin-modified titanium sheet can maintain its antibacterial biological activity and can promote osteogenic differentiation and bone mineralization of bone marrow mesenchymal stem cells. In addition, it has the function of inhibiting the secretion of inflammatory factors, and the preparation process is simple, the reaction conditions are mild, the efficiency is high, the cost is low, and the repeatability is good. The invention can effectively improve the defect that the current titanium alloy is applied to clinical biological function only, and reduce related complications such as infection and early loosening caused by excessive inflammatory response after the titanium alloy is implanted in the body.

Description

technical field [0001] The invention relates to the technical field of orthopedic prosthesis, in particular to a bacitracin-fixed titanium alloy prosthesis for anti-inflammation, anti-infection and promotion of osteogenic differentiation and bone mineralization and a preparation method thereof. Background technique [0002] A perfect orthopedic implant should avoid complications after implantation. Zhao (Zhao, Journal of biomedical materials research Part B, Applied biomaterials 2009, 91(1):470-480) proposed that antibacterial coating is an important means to improve the infection of internal plants. Zhang (Zhang, Int J Mol Sci 2014,15(7):11878-11921.) believes that implants that promote osseointegration and resist infection have the ability to reduce the risk of aseptic and septic loosening. Goodman (Goodman SB, Biomaterials 2013,34(13):3174-3183) proposed that the future focus of orthopedic biocoating implants should be to improve osseointegration, reduce infection, and r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/32A61L27/22A61L27/06A61L27/54
Inventor 岳冰聂彬恩敖海勇
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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