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Ganoderma lucidum spore wall breaking method

A technology of Ganoderma lucidum spores and wall-broken Ganoderma lucidum spore powder is applied in the directions of biochemical equipment and methods, the dissolution of microorganisms, and the treatment of microorganisms with electricity/wave energy. Efficacy, low rate of Ganoderma lucidum spore wall breaking, etc., to achieve the effect of being conducive to effective separation, better clinical effect, and high dissolution rate

Inactive Publication Date: 2016-01-06
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing methods, the wall breaking rate of ganoderma lucidum spores is not high, the active ingredients cannot be fully absorbed, and it is difficult to reflect the multi-component efficacy of ganoderma lucidum spores

Method used

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  • Ganoderma lucidum spore wall breaking method
  • Ganoderma lucidum spore wall breaking method
  • Ganoderma lucidum spore wall breaking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A method for breaking walls of ganoderma lucidum spores, comprising the following steps:

[0051] (1) Immobilization and catalytic reaction of compound enzyme

[0052] Weigh 5.0g of sodium alginate into a beaker of 150mL distilled water, heat until boiling and completely dissolve, then place room temperature and gradually cool to about 35°C, add cellulase, chitinase, dextranase prepared in advance (weight ratio 3:1:1) solution 100mL.

[0053] (2) Extract the sodium alginate-enzyme mixture with a syringe, slowly drop the mixture into a beaker containing 500mL of 3.0% calcium chloride solution, and at the same time place the beaker on a magnetic stirrer to stir at a constant speed, and let it stand for 30 minutes to harden after dropping , pour off the calcium chloride solution, wash with appropriate amount of distilled water for 3-4 times, remove the floating cavitation beads, and then prepare the immobilized complex enzyme.

[0054] Put the immobilized compound enzyme...

Embodiment 2

[0057] Embodiment 2 A method for breaking walls of Ganoderma lucidum spores, comprising the following steps:

[0058] (1) Immobilization and catalytic reaction of compound enzyme

[0059] Weigh 5.0g of agar into a 150mL beaker of distilled water, heat it to boiling and dissolve completely, place it at room temperature and gradually cool it to about 35°C, add cellulase, chitinase, and dextranase prepared in advance (weight ratio 3 :1:1) solution 100mL.

[0060] (2) Extract the sodium alginate-enzyme mixture with a syringe, slowly drop the mixture into a beaker containing 500mL of 3.0% calcium chloride solution, and at the same time place the beaker on a magnetic stirrer to stir at a constant speed, and let it stand for 30 minutes to harden after dropping , pour off the calcium chloride solution, wash with appropriate amount of distilled water for 3-4 times, remove the floating cavitation beads, and then prepare the immobilized complex enzyme.

[0061] Put the immobilized comp...

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Abstract

The invention discloses a ganoderma lucidum spore wall breaking method. According to the ganoderma lucidum spore wall breaking method, pretreatment is performed by using immobilized compound enzyme first, and then broken-wall ganoderma lucidum spore powder is obtained by adopting a superfine pulverization technology, ultrasonic waves or a homogenizing method through processing. The ganoderma lucidum spore wall breaking method is a processing method which is optimized by screening different processing processes through a large number of experiments and integrates with the immobilized compound enzyme method, the superfine pulverization technology and other technologies, and the wall breaking rate within a short time can be up to 100%. In addition, effective ingredients including total triterpenes, total polysaccharides and the like are higher in dissolution rate and are easier to absorb in clinical application, the clinic effect is better, the whole process is reasonable in design and convenient to operate, industrial production can be achieved, and the ganoderma lucidum spore wall breaking method has important promotional value and economic benefit.

Description

technical field [0001] The invention relates to a processing method of traditional Chinese medicine, in particular to a method for breaking the wall of ganoderma lucidum spores with high wall-breaking rate and higher active ingredient content. Background technique [0002] Ganoderma lucidum spores are germ cells ejected from the fruiting bodies of Ganoderma lucidum in the later stage of development. They are light brown to yellowish brown, oval, double-walled, and the inner spore wall is light brown to yellowish brown with protruding thorns. The outer spore wall is smooth and without color. Ganoderma lucidum spores contain active ingredients such as polysaccharides, triterpenoids, fatty acids, amino acids, polypeptides, sterols, and inorganic ions, which are more abundant than the fruiting bodies of Ganoderma lucidum. Ganoderma lucidum spores are rich in polysaccharides, and the protein content is as high as 18%, among which isoleucine, lysine, valine, methionine and other ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12N13/00
Inventor 江曙钱大玮郭建明赵敏
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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