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Tungsten-oxide-quantum-dot-material simulated peroxidase

A technology of tungsten oxide quantum dots and peroxidase, applied in tungsten oxide/tungsten hydroxide, physical/chemical process catalyst, analysis by chemical reaction of materials, etc., can solve easy deactivation, purification difficulty, limitation application and other problems, to achieve the effect of good peroxidase activity, simple preparation process and cost reduction

Active Publication Date: 2016-01-06
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Although natural enzymes have the characteristics of high catalytic efficiency, strong specificity, and mild reaction conditions, the activity of natural enzymes is easily affected by environmental factors such as pH value, temperature, and toxicity, so they have disadvantages such as instability and easy inactivation.
In addition, natural enzymes also have disadvantages such as limited sources, difficult purification, and high price, which greatly limit their applications.

Method used

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  • Tungsten-oxide-quantum-dot-material simulated peroxidase
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  • Tungsten-oxide-quantum-dot-material simulated peroxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 0.03g of tungsten sulfide, add 200mL of double-distilled water at 65°C, and sonicate in an ultrasonic cleaner (power 400W) for 15-24 hours (preferably 18 hours). Filter through a sand core funnel with a pore size of 0.22 μm to obtain a filter cake, and then resuspend the collected filter cake in 30 mL of twice distilled water. The above solution is placed in a polytetrafluoroethylene high-pressure reactor, reacted at 200°C for 12-18 hours (preferably 15 hours), and then cooled to room temperature to obtain a hydrothermal product. The obtained hydrothermal product is filtered through a sand core funnel with a pore size of 0.22 μm, and the filtrate is collected to obtain a tungsten oxide quantum dot material solution. After suspension steaming, ethanol dispersion, centrifugation (centrifuge speed is 16000r / min, time 30min), vacuum drying (temperature is 50°C-60°C (preferably 55°C), drying time is 8h-12h (preferably 10 hours) )) step to obtain tungsten oxide quantum...

Embodiment 2

[0032] Add 0.2mL of 3% hydrogen peroxide and 0.2mL of 4mmol / L 3,3',5,5'-tetramethylbenzidine to 0.5mLHAc-NaAc buffer (pH3.6, 20mmol / L) Hydrochloric acid and 100 μL of the tungsten oxide quantum dot material aqueous solution prepared in Example 1 with a concentration of 10 μg / mL were mixed and incubated at 37°C for 30 minutes, and the solution changed from colorless to blue (see figure 1 , left), with an absorption peak at 652nm (see figure 2 ).

Embodiment 3

[0034] Add 0.2mL of 3% hydrogen peroxide and 0.2mL of 4mmol / L 2,2'-azino-bis(3-ethylbenzene And thiazoline-6-sulfonic acid) diammonium salt and 100 μ L concentration are the tungsten oxide quantum dot material aqueous solution that the embodiment 1 of 10 μ g / mL makes, after mixing, the solution changes from colorless to green immediately (see figure 1 , middle image).

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Abstract

The invention discloses tungsten-oxide-quantum-dot-material simulated peroxidase. A tungsten oxide quantum dot material has excellent peroxidase activity and can catalyze color developing agents such as hydrogen-peroxide-oxidized 3,3',5,5'-tetramethyl benzidine hydrochloride, diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) and o-phenylenediamine to develop colors. In this way, a novel simulated peroxidase inorganic nano-material capable of replacing natural enzymes is provided. The tungsten oxide quantum dot material is prepared in the mode that tungsten sulfide serves as a precursor, and reacting is carried out through a simple ultrasonic step and a hydrothermal method. The material is simple in synthesis step, environmentally friendly and low in cost; in addition, the peroxidase simulating effect is obvious, and therefore good application prospects are achieved in novel catalytic oxidation and medical analysis.

Description

technical field [0001] The invention relates to a novel simulated peroxidase based on tungsten oxide quantum dot material, which belongs to the technical field of simulated catalytic oxidation. Background technique [0002] Although natural enzymes have the characteristics of high catalytic efficiency, strong specificity, and mild reaction conditions, the activity of natural enzymes is easily affected by environmental factors such as pH value, temperature, and toxicity, so they have disadvantages such as instability and easy inactivation. In addition, natural enzymes also have disadvantages such as limited sources, difficult purification, and high price, which greatly limit their applications. In recent years, the emergence of nanomaterials to artificially simulate peroxidase can effectively overcome the above shortcomings of natural enzymes, showing strong catalytic activity and specificity, and has the advantages of simple preparation, low price, good stability, and reusab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/30C01G41/02G01N21/78G01N21/31
Inventor 彭花萍刘爱林陈伟林新华刘盼林丹薇
Owner FUJIAN MEDICAL UNIV
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