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Rheumatoid factor detection reagent

A technology for rheumatoid factor and detection reagents, which is applied in measurement devices, instruments, disease diagnosis, etc., can solve problems such as poor prognosis of patients, and achieve the effect of improving detection performance, accuracy and stability, and meeting clinical needs.

Active Publication Date: 2015-12-23
郁东
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the detection range of the main RF detection kits on the market is 0-80IU / mL. Studies have shown that if the titer of IgM type RF in the serum of RA patients> 80IU / mL, and with severe joint dysfunction, this indicates that the patient's prognosis is poor

Method used

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Embodiment 1

[0038] A rheumatoid factor detection reagent is composed of reagent R1 and reagent R2;

[0039] Said, reagent R1 is made up of following components:

[0040] Phosphate buffer 50mmol / L

[0041] Lauryl imidazoline betaine 1ml / L-5ml / L

[0042] Dioctadecyldimethylammonium chloride 0.1ml / L-1ml / L

[0043] Dodecyltrimethylammonium chloride 0.1g / L-1g / L

[0044] Alkylphenol polyoxyethylene ether (APEO) 2g / L

[0045] Hydroxyethylidene diphosphonic acid 1ml / L-5ml / L

[0046] Sodium azide 0.095%

[0047] Stabilizer SHE-500.15%

[0048] Described, reagent R2 is made up of following components:

[0049] Phosphate buffer 50mmol / L

[0050] Thermally polymerized I human IgG30ml / L

[0051] Latex Microspheres 5%

[0052] Kathon-CG0.05%

[0053] Alkylphenol polyoxyethylene ether (APEO) 2g / L

[0054] Sodium azide 0.095%.

[0055] As mentioned, the volume ratio of reagent R1 and reagent R2 is 4:1.

[0056] Said, the pH of the phosphate buffer is 7.4.

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Abstract

The present invention discloses a rheumatoid factor detection reagent, which comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a phosphate buffer solution, lauryl imidazolinium betaine, dioctadecyl dimethyl ammonium chloride, dodecyl trimethyl ammonium chloride, alkyl phenol ethoxylates (APEO), 1-hydroxyethylidene-1,1-diphosphonic acid, sodium azide and a stabilizer SHE-50, and the reagent R2 comprises a phosphate buffer solution, thermal polymerization I human IgG, latex microspheres, Kathon-CG, alkyl phenol ethoxylates (APEO) and sodium azide. According to the present invention, a variety of the reagents in the detection reagent of the present invention provide the synergy effect, and the detection reagent has characteristics of good accuracy, good stability, wide linear range, cheap price and easy use, and can completely meet the clinical needs.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a rheumatoid factor detection reagent with good accuracy and wide linear range. Background technique [0002] Rheumatoid factor (rheumatoid factor, RF) detection is a relatively common detection item in clinical practice, mainly used in the diagnosis of autoimmune diseases, especially the diagnosis of rheumatoid arthritis. Rheumatoid factor (RF) is an autoantibody with denatured IgG as the target antigen, which is caused by hemolytic streptococcus or other bacterial metabolites, chronic viruses and other infectious agents. RF was first discovered by Rose and Waller (1984) in the serum of patients with rheumatoid arthritis (RA). RF is mainly 19S IgM, which has poor binding ability to natural IgG, and is most likely to bind to denatured IgG of humans and animals or IgG in immune complexes. Studies have shown that the positive rate of RF is only 2% in ...

Claims

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Application Information

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IPC IPC(8): G01N33/564
CPCG01N33/564G01N2800/102
Inventor 郁东高雪萍甘宜梧谢清华谭柏清
Owner 郁东
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