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Trichoderma viride strain and application thereof

A technology of Trichoderma viride and bacterial strains is applied in the field of microorganisms, which can solve the problems of chemical control of environmental pollution and unsafety, and achieves the effects of simple and practical process, high control effect and stable control effect.

Active Publication Date: 2015-12-16
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Trichoderma viride bacterial strain and its application

Method used

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  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation, purification and identification of Trichoderma viride:

[0028] Take soil samples evenly from the tomato planting fields in Shunyi, mix each 10g of soil with 100ml of sterilized water to make a suspension, and then perform gradient dilutions in sequence, take 10000 times diluted soil mixture 100μl and spread it on the PDA medium plate (i.e. 200 g of potatoes, 20 g of glucose, 17 g of agar, and distilled water to a volume of 1000 ml. This culture medium was sterilized at 121° C. and spread into a plate in a petri dish). PDA medium contains 75 μg / ml ampicillin, which inhibits bacterial growth. The culture medium was cultured at 25°C. After the tissue pieces grew colonies, single spores were isolated, and single colonies were picked and purified on PDA medium for classification and identification. The strains were morphologically determined according to the Fungal Identification Manual.

[0029] Pick the mycelium on the colony, put it into an EP tube containin...

Embodiment 2

[0033] Preparation and application of Trichoderma viride seed coating agent:

[0034] The Tvir-6 strain was inoculated into 250ml Erlenmeyer flasks, each bottle containing 125ml of PDB liquid medium, and cultured at 25°C for 8 days with shaking (160rpm) to obtain a large number of mycelia and conidia. The liquid fermentation medium is a potato dextrose liquid (PDB) medium, that is, 200 g of potatoes, 20 g of glucose, and distilled water to a volume of 1000 ml.

[0035] The spore mycelium mixed solution obtained by liquid shaking culture (fermentation), the spore concentration is 10 7 pcs / ml), add the following ingredients: the ratio is as follows: 1L of spore liquid, 500g of diatomaceous earth, 200g of corn flour, and 50g of wheat bran. The solid fermented product is placed at room temperature and naturally dried for 3 days (the water content of the fermented product is controlled between 15-25%), crushed and passed through a 60-mesh sieve to obtain spore powder. The spore p...

Embodiment 3

[0037] Preparation and application of Trichoderma viride seed coating agent:

[0038] The Tvir-6 strain was inoculated into 250ml Erlenmeyer flasks, each bottle containing 125ml of PDB liquid medium, and cultured at 28°C for 6 days with shaking (160rpm) to obtain a large number of mycelia and conidia. The liquid fermentation medium is a potato dextrose liquid (PDB) medium, that is, 200 g of potatoes, 20 g of glucose, and distilled water to a volume of 1000 ml.

[0039] The spore mycelium mixed solution obtained by liquid shaking culture (fermentation), the spore concentration is 10 10pcs / ml), add the following ingredients: the ratio is as follows: 2L of spore liquid, 700g of diatomaceous earth, 400g of corn flour, and 150g of wheat bran. The solid fermented product is placed at room temperature to dry naturally for 5 days (the water content of the fermented product is controlled between 15-25%), crushed, and passed through a 60-mesh sieve to obtain spore powder. The spore po...

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Abstract

The invention discloses a Trichoderma viride strain and application thereof. The Trichoderma viride strain Tvir-6 is collected by China General Microbiological Culture Collection Center, and the collection number is CGMCC NO.10922. The invention also discloses application of a biocontrol seed coating prepared from the Trichoderma viride strain Tvir-6 in controlling Meloidogyne. The Trichoderma viride Tvir-6 strain is a fungus separated from soil, is safe and harmless to crops, has a control action on Meloidogyne, has high safety in application, and is suitable for biological control of Meloidogyne. The lethality rate of the Trichoderma viride Tvir-6 strain fermentation liquid for Meloidogyne second instar larvae is up to 99% or above, and the control effect in a greenhouse is up to 79.5%; and the Trichoderma viride Tvir-6 strain has high and stable control effect, and is suitable for agricultural production demands.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Trichoderma viride and its application. Background technique [0002] Root-knot nematode (Meloidogyne) is a highly specialized omnivorous plant pathogenic nematode. Nematodes known to harm vegetables mainly include root-knot nematode peanut, root-knot nematode northern, root-knot nematode southern, root-knot nematode java, and root-knot nematode beet. Nematodes have a wide range of hosts, often harming more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, carrots, lettuce, and cabbage, and can also spread some fungal and bacterial diseases. Root-knot nematode is an important crop pathogen, causing losses in the order of billions of dollars each year. Root-knot nematodes mainly infect the roots of crops. After infection, the roots swell to form huge root knots, which can be connected to form beads. Because the roots are damaged,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/885
Inventor 茆振川马玉琴张晓平凌键陈国华杨宇红谢丙炎
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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