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Method of simultaneously extracting caffeic acid and ferulic acid in cimicifuga rhizome

A technology of caffeic acid and ferulic acid, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of low selectivity, low recovery rate, low product purity, etc. Degree, the effect of strong practical value

Active Publication Date: 2015-12-09
河北汇正生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects of low selectivity, low recovery rate and low product purity in the existing complex sample extraction technology, and provide a method for molecularly imprinted solid-phase extraction of the target substance, through metal ions, multiple Template molecules, functional monomers are effectively combined and then imprinted to obtain a Zn 2+ - Ferulic acid-caffeic acid coordinate imprinted polymer, and use it as a solid phase extraction material to simultaneously extract ferulic acid and caffeic acid in Cimicifuga

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of molecularly imprinted materials: Weigh the template molecule ferulic acid-caffeic acid mixture and zinc acetate, dissolve in the acetonitrile-dimethyl sulfoxide mixed solution and shake for 10 minutes, add the functional monomer 4-VP, shake for 30 minutes, and make It fully interacts with functional monomers, then add mixed cross-linking agent EDMA-DVB and initiator AIBN, after fully mixing, transfer to a round-bottomed flask, ultrasonic degassing for 15 minutes, and argon gas for 15 minutes, and seal the bottle; put the round-bottomed flask Put it in a constant temperature water bath at 60°C for 24 hours of polymerization reaction, pulverize the obtained massive polymer, pass through a 200-mesh sieve, settle with tetrahydrofuran, methanol and water in turn, remove the fine particles in the upper layer of the solution, filter, and use it under ultrasonic conditions 0.10mmol / LEDTA washes away the metal ions in the polymer, washes with methanol-acetic ac...

Embodiment 2

[0018] (1) Preparation of molecularly imprinted materials: Weigh the template molecule ferulic acid-caffeic acid mixture, dissolve zinc acetate in acetonitrile-dimethyl sulfoxide mixed solution and shake for 10 minutes, add functional monomer 4-VP, shake for 30 minutes, and make It fully interacts with functional monomers, then add mixed cross-linking agent EDMA-DVB and initiator AIBN, after fully mixing, transfer to a round-bottomed flask, ultrasonic degassing for 15 minutes, and argon gas for 15 minutes, and seal the bottle; put the round-bottomed flask Put it in a constant temperature water bath at 60°C for 24 hours of polymerization reaction, pulverize the obtained massive polymer, pass through a 200-mesh sieve, settle with tetrahydrofuran, methanol and water in turn, remove the fine particles in the upper layer of the solution, filter, and use it under ultrasonic conditions 0.10mmol / LEDTA washes away the metal ions in the polymer, washes with methanol-acetic acid (9 / 1, v / v...

Embodiment 3

[0022] (1) Preparation of molecularly imprinted materials: Weigh the template molecule ferulic acid-caffeic acid mixture, dissolve zinc acetate in acetonitrile-dimethyl sulfoxide mixed solution and shake for 10 minutes, add functional monomer 4-VP, shake for 30 minutes, and make It fully interacts with functional monomers, then add mixed cross-linking agent EDMA-DVB and initiator AIBN, after fully mixing, transfer to a round-bottomed flask, ultrasonic degassing for 15 minutes, and argon gas for 15 minutes, and seal the bottle; put the round-bottomed flask Put it in a constant temperature water bath at 60°C for 24 hours of polymerization reaction, pulverize the obtained massive polymer, pass through a 200-mesh sieve, settle with tetrahydrofuran, methanol and water in turn, remove the fine particles in the upper layer of the solution, filter, and use it under ultrasonic conditions 0.10mmol / LEDTA washes away the metal ions in the polymer, washes with methanol-acetic acid (9 / 1, v / v...

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Abstract

The invention discloses a method of simultaneously extracting caffeic acid and ferulic acid in cimicifuga rhizome. The method comprises the following steps: firstly, weighing according to certain ratio and dissolving ferulic acid, caffeic acid and zinc acetate in an acetonitrile-dimethyl sulfoxide mixed solution, vibrating, adding a functional monomer 4-VP, sufficiently vibrating, then adding a mixed cross-linking agent EDMA-DVB and an initiator AIBN, sufficiently mixing the raw materials, transferring the raw materials into a round-bottom flask, degassing, feeding argon, and sealing the bottle; secondly, laying the bottle into a 60 DEG C thermostat water bath, performing crushing after the completion of a polymerization reaction, and performing sieving, settling, filtering, eluting and vacuum drying; thirdly, loading an obtained molecularly imprinted polymer into a solid-phase extraction tube, and performing washing and eluting after the loading. The method adopts a metal ion coordination imprinting technology, takes a binary compound metal ion complex as template molecules, synthesizes the molecularly imprinted polymer with high selective recognition property, and has high practical value by simultaneously extracting the caffeic acid and the ferulic acid in the cimicifuga rhizome through the molecularly imprinted polymer.

Description

technical field [0001] The invention relates to a method for simultaneously extracting caffeic acid and ferulic acid in Cimicifuga by using a newly prepared molecular imprinted material. Background technique [0002] Molecularly imprinted technology aims to construct supramolecular compounds with high selectivity and even specific binding ability to target compounds. Due to the matching of the binding site with the target molecule in terms of shape, size and chemical functional groups, molecularly imprinted polymers can be used It is used for selective enrichment and even specific identification of target compounds in complex systems. So far, molecular imprinting technology has been widely used in solid phase extraction, chromatographic separation, sensing technology, immunoassay and simulated enzyme catalysis and other fields. However, most studies on molecular imprinting in the past mainly focused on the imprinting preparation and molecular recognition of a single templat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/47C07C59/64C07C59/52
CPCC07C51/47C07C59/64C07C59/52
Inventor 李辉王素素张月
Owner 河北汇正生物科技有限公司
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