Efficient culture medium for achieving efficient cultivation of armillaria mellea by improving extracellular enzyme activity thereof and preparation method therefor

Abstract

The present invention discloses an efficient culture medium for achieving efficient cultivation of armillaria mellea by improving extracellular enzyme activity thereof. The efficient culture medium comprises the following components in parts by weight: polycaprolactone microspheres, porous bioactive calcium phosphate microspheres, pine needle powder, edible fungus residue, cucurmosin, expanded rice bran husks, yeast cell walls, and yeast. According to the efficient culture medium provided by the invention, the pine needle power is subjected to hydrolysis performed by modified cellulases, and is used in combination with the edible fungus residue and the cucurmosin. The expanded rice bran husks are fermented to prepare the armillaria mellea culture medium, so that reasonable combination and abundant nutrition can improve the extracellular enzyme activity of the armillaria mellea, thereby improving cultivation efficiency of an extracellular enzyme. The used modified cellulases exist in the culture medium for a long time so as to decompose cellulose continuously and collaboratively and provide nutrition for the armillaria mellea. The porous bioactive calcium phosphate microspheres are used to adsorb the loaded yeast for fermentation, so that activity of the yeast is increased and decomposition of the culture medium raw material is promoted, thereby facilitating absorption and application, and providing a foundation for efficient cultivation of the armillaria mellea.

Description

technical field [0001] The invention relates to the field of edible fungus culture medium, in particular to a high-efficiency medium and a preparation method thereof for realizing high-efficiency cultivation of Armillaria armillaria by increasing the activity of extracellular enzymes. Background technique [0002] Armillaria, also known as clam mushrooms, is a facultative parasitic fungus belonging to the genus Armillaria in the family Agaricaceae. It is an important edible fungus and the main source of nutrition to promote the development of Tianma and Polyporus. Armillaria can not directly use macromolecular nutrients in the culture medium, it needs to be degraded into small molecules by extracellular enzymes for absorption. CMC enzyme belongs to cellulase to decompose cellulose, xylanase can decompose hemicellulose, honey ring The extracellular laccase is the main enzyme produced in the secondary growth and metabolism stage of Armillaria armillaria, and the regulation of ...

AI Technical Summary

Problems solved by technology

[0003] The micropores of the microspheres with a porous structure cover the entire microsphere and go deep into the center from the surface. When used as a carrier, it has the characteristics of many adsorption sites, large specific surface area and strong adsorption capacity, which can change the load. Object solubility, release ability and other characteristics are often used as carriers for slow-release fertilizers; in the preparation process of high-efficiency submerged fermentation medium for edible fungus cultivation, the decomposition and fermentation of medium raw materials directly determine the quality of the medium, and then Determine the effect of using gold in cultivation; the control of related enzymes and fermentation bacteria activity is the key and difficulty in the preparation of high-efficiency submerged fermentation medium. At present, there is no porous structure microsphere as the control of related enzymes and fermentation bacteria activity in the production process of the medium. According to reports, but according to the large specific surface area of ​​porous microspheres, it is conducive to the adhesion of a large number of cells, the transport of nutrients, the discharge of metabolites and the interaction between cells, which should be used in enzymes and fermentation. control of bacterial activity