Construction method of contrast agent induced acute kidney injury animal model

Abstract

The invention provides a construction method of a contrast agent induced acute kidney injury animal model. A clinically commonly used low permeability contrast agent is used in the construction method to make the stable CI-AKI model within a short time, so interferences of other confounding factors are effectively avoided, bases are provided for further researches of the pathologic and physiologic mechanisms, the molecular cytology and the molecular biology of the CI-AKI, and bases are provided for early prevention and treatment of CI-AKI of clinic patients.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing an animal model of acute kidney injury caused by a contrast agent. Background technique [0002] With the wide application of contrast-enhanced CT and interventional diagnosis and treatment in the clinic, the number of people using contrast agents in China reaches 20-30 million per year, and contrast-induced acute kidney injury (Contrast-Induced acute kidney injury, CI-AKI) The incidence rate is 1%-5%, about 200,000 to 1 million cases of CI-AKI occur each year, CI-AKI significantly increases the mortality rate, as high as 9.7-14%, the hospitalization time is longer, and the medical expenses increase, which brings huge to the society economic and social burden. In 2013, the national interventional treatment registered case data showed that the number of PCI cases had exceeded 450,000, and the number of coronary artery diagnosis and treatment p...

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Problems solved by technology

[0002] With the wide application of contrast-enhanced CT and interventional diagnosis and treatment in the clinic, the number of people using contrast agents in China reaches 20-30 million per year, and contrast-induced acute kidney injury (Contrast-Induced acute kidney injury, CI-AKI) The incidence rate is 1%-5%, about 200,000 to 1 million cases of CI-AKI occur each year, CI-AKI significantly increases the mortality rate, as high as 9.7-14%, the hospitalization time is longer, and the medical expenses increase, which brings huge to the society economic and social burden

In 2013, the national interventional treatment registered case data showed that the number of PCI cases had exceeded 450,000, and the number of coronary artery diagnosis and treatment patients was close to 2 million, but the average number of PCI cases per million population was 309.42 cases, while the United States was about 1,800 cases / million people. Therefore, the number of coronary interventional diagnosis and treatment cases in my country will continue to increase, and the risk of CI-AKI is still increasing. In addition, some studies have found that for people with high-risk factors, such as underlying renal insufficiency, diabetes, congestive heart failure, and hypertension , advanced age, etc., the incidence of CI-AKI further increases, but the pathophysiological mechanism of CI-AKI is still not completely clear, and there is no effective treatment

[0007] Among the CI-AKI models involved in the above basic studies, the method of establishing the CI-AKI model needs to be established through surgery. The operation is complicated and invasive, and the waiting time after the operation is long, which is not conducive to generalization; in addition, based on Although the CI-AKI model established by combined dehydration, injection of non-steroidal drugs such as indomethacin and hyperosmolar contrast media is superior to the model of surgical methods, the current clinically relevant guidelines have clearly suggested that patients should be exposed to contrast media Before discontinuing nephrotoxic drugs, including non-steroidal drugs and aminoglycoside antibiotics, etc.

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