Uses of Rhododendron Extract
A plant extract, the technology of Aurantia genus, applied in the direction of dressing preparations, skin care preparations, pharmaceutical formulations, etc., can solve the problems of freckle removal, whitening, wrinkle removal, sun protection or anti-aging of plants of the genus Aurantia genus. question
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Embodiment 1
[0062] The dried leaves of Callicarpa cathayana H.T.Chang were crushed to the coarsest powder, and extracted by percolation with 90% ethanol at a flow rate of 1 mL / h per gram of dried leaf powder of Callicarpa cathayana H.T.Chang for 10 h. The percolation liquid is collected, and the percolation liquid is concentrated under reduced pressure to remove ethanol, and the volume of the concentrated liquid is equivalent to the amount of medicinal materials. Add double the volume of water, let it stand overnight, and centrifuge to obtain the supernatant. Make the supernatant pass through the D101 macroporous adsorption resin column, wash the impurities with water of 6 times the bed volume of the resin column, discard the washing solution, and then elute with 40% ethanol of 3 times the bed volume of the resin column, collect eluent. The eluate was concentrated under reduced pressure to remove ethanol. Dilute the concentrated solution with water to the volume of the initial supernata...
Embodiment 2
[0064] Crush the dried stems and leaves of Callicarpa cathayana H.T.Chang to the coarsest powder, add 12 times the amount (that is, 12ml / g dried stems and leaves of Callicarpa cathayana H.T.Chang) of 80% ethanol, and flash extract for 3 minutes. Extract twice, combine the extracts, concentrate under reduced pressure to remove ethanol, the volume of the concentrate is equivalent to the amount of medicinal materials. Add double the volume of water, let it stand overnight, and centrifuge to obtain the supernatant. Make the supernatant pass through the D101 macroporous adsorption resin column, wash with water of 6 times the bed volume of the resin column, discard the washing solution, elute with 30% ethanol of 3 times the bed volume of the resin column, and collect the eluted liquid. The eluate was concentrated under reduced pressure to remove ethanol. Dilute the concentrated solution with water to the volume of the initial supernatant, then pass through the polyamide chromatogr...
Embodiment 3
[0066] Crush the dried leaves of Callicarpa formosana Rolfe to the coarsest powder, add 12 times the amount (ie, 12ml / g dry leaf powder of Callicarpa formosana Rolfe) of 70% ethanol, and perform flash extraction for 3 minutes, and the extraction frequency is 2 times. Combine the two extracts, concentrate the extract under reduced pressure to remove ethanol, and the volume of the concentrate is equivalent to the amount of medicinal materials. Add double the volume of water, let it stand overnight, and centrifuge to obtain the supernatant. Make the supernatant pass through the D101 macroporous adsorption resin column, wash with water of 6 times the bed volume of the resin column, discard the washing solution, elute with 30% ethanol of 4 times the bed volume of the resin column, and collect the eluted liquid. Concentrate the eluent under reduced pressure and dry to obtain the extract of Rhododendron flower leaf as a tan uniform powder.
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