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Method for preventing and curing Bradysia odoriphaga by jointly applying entomopathogenic nematodes and chemical pesticides

A technology of entomopathogenic nematodes and chemical pesticides, which is applied in the field of biological control and chemical control, can solve the problems of instability, slow killing of entomopathogenic nematodes, etc., and achieve the effect of reducing the amount of use, improving the control effect, and safe leek maggots

Inactive Publication Date: 2015-11-25
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, when using entomopathogenic nematodes for biological control, not only field temperature, soil moisture, soil structure, natural enemies, solar ultraviolet rays and other factors need to be considered, but also the effective dose of nematodes, cold resistance, drought resistance, and exercise ability, etc., must be considered. These factors make entomopathogenic nematode killing slow and erratic

Method used

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  • Method for preventing and curing Bradysia odoriphaga by jointly applying entomopathogenic nematodes and chemical pesticides
  • Method for preventing and curing Bradysia odoriphaga by jointly applying entomopathogenic nematodes and chemical pesticides
  • Method for preventing and curing Bradysia odoriphaga by jointly applying entomopathogenic nematodes and chemical pesticides

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Experimental program
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Effect test

Embodiment 1

[0023] The impact of embodiment 1 chemical pesticide on entomopathogenic nematode survival

[0024] Heterorhabditis bacteriophora Cangzhou strain (CZ for short) was collected and isolated from the peanut planting area in Cangzhou, Hebei Province, and was propagated live with Galleria mellonella to obtain infective juvenile stages (IJs). The larvae of Mellonella mellonella used in the experiment were artificially reared indoors in our laboratory. All the tested pesticides were provided by the Pesticide Laboratory of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0025] Select 70% imidacloprid (Imidacloprid), 40% chlorpyrifos (Chlorpyrifos), 35% phoxim (Phoxim) and 25% thiamethoxam (Thiamethoxam) commonly used in the leek field as test chemical pesticides. , RD) is diluted 4000 times, and the recommended dosage of each pesticide is diluted 1 / 2 times (referred to as 1 / 2RD), 1 / 4 times (referred to as 1 / 4RD), 1 / 8 times (referred to as 1 / 8RD) as the ...

Embodiment 2

[0035] Example 2 Effect of Chemical Pesticides on Entomopathogenic Nematode Infection Ability

[0036] Shake the mixed solution of medicament and entomopathogenic nematode suspension or the mixed solution of clear water and pathogenic nematode suspension in Example 1, and evenly drop it in a petri dish (Φ=9cm) that is cushioned with two layers of medium-speed qualitative filter paper (double circle brand) Add 2mL of the mixed solution, place it in the dark at 25±1°C, and pick 5 last-instar larvae of Mellonella mellonella 24 hours later. Four replicates were set up for each treatment, and nematodes were treated alone as a control. After 72 hours of treatment, it was observed whether the larvae of Mellonella mellonella died. The dead worms treated with nematodes were taken out and placed in a clean petri dish, and dissected under a stereomicroscope to observe whether there were nematodes in the dead S. mellonella moth, and record the number of S. mellonella moth larvae infected b...

Embodiment 3

[0041] Embodiment 3, the lethal effect of imidacloprid and entomopathogenic nematodes in combination on leek maggots

[0042] The dosage of imidacloprid and the suspension of entomopathogenic nematodes are the same as in Example 1. The leek maggots (Bradysiaodoriphaga Yanget Zhang) for the test were provided by the Institute of Plant Protection, Tianjin Academy of Agricultural Sciences (long-term artificial rearing after collection in local leek fields in Tianjin).

[0043] Put a piece of medium-speed filter paper in a petri dish (Φ=9cm) equipped with 2.5% agar, add 0.6 mL of distilled water dropwise, and put 20 4th-instar tardyophthora larvae and appropriate amount of chive stems. Add 0.1mL of nematode solution (2000IJs) and 0.1mL of different doses of imidacloprid liquid respectively, and set imidacloprid and entomopathogenic nematode single-use treatment, and distilled water treatment as control. Place them in a constant temperature incubator at (22±1°C), check and record ...

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Abstract

The invention discloses a method for preventing and curing Bradysia odoriphaga by jointly applying entomopathogenic nematodes and chemical pesticides and belongs to the technical field of biological control and chemical control. At the 3-4 days old of the Bradysia odoriphaga, an entomopathogenic nematode resin agent and one-eighth (recommended dosage) of the chemical pesticides, which are safe to the nematodes, are jointly used to prevent and cure the Bradysia odoriphaga. According to the control method disclosed by the invention, by jointly using biological control and chemical control, the amount of use of the entomopathogenic nematodes and the chemical pesticides is reduced, the cost of prevention and cure is reduced, the control effect of the entomopathogenic nematodes and the chemical pesticides on the Bradysia odoriphaga is improved, and thus the aim of efficiently and safely preventing and curing the Bradysia odoriphaga is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological control and chemical control, and in particular relates to a method for jointly controlling chive maggots by applying entomopathogenic nematodes and chemical pesticides. Background technique [0002] Bradysiaodoriphaga Yanget Zhang, the larvae of Bradysiaodoriphaga Yanget Zhang, are commonly known as chive maggots. Chive larvae are mainly infested by larvae. Chive maggots feed on a wide range, and can harm more than 30 kinds of vegetables in 7 families, including Liliaceae, Chenopodiaceae, Compositae, Cucurbitaceae, Cruciferae, and Umbelliferae, among which leeks are affected. The most serious, followed by garlic, onion, lettuce, melons and mushrooms; in the main production areas of leeks in my country, the occurrence of this insect is relatively common, with an occurrence area of ​​up to 90%. The rate of damaged plants in the leek field is generally above 20%, resulting in a loss of 30% to 80%...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P7/04A01G1/00A01G13/00A01N51/00A01N57/14A01N57/16
Inventor 李克斌尹姣张帅梅向东曹雅忠吴文丹张开心
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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