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Complex enzyme preparation for extracting effective plant components and method for preparing complex enzyme preparation

A technology of plant active ingredients and compound enzyme preparations, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of complex production processes, inconvenient mastery, and large-scale production

Inactive Publication Date: 2015-11-18
天津科建科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used enzymes are single liquid dosage forms, and the enzymes must be added in parts, so the production process is complicated, and it is inconvenient to master and large-scale production

Method used

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  • Complex enzyme preparation for extracting effective plant components and method for preparing complex enzyme preparation
  • Complex enzyme preparation for extracting effective plant components and method for preparing complex enzyme preparation
  • Complex enzyme preparation for extracting effective plant components and method for preparing complex enzyme preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of Plant Extract Compound Enzyme Preparation

[0062] (1) The source of each strain:

[0063] Bacillus licheniformis Bacillus licheniformis , No. CGMCC1970;

[0064] Bacillus subtilis Bacillus subtilis , No. AS1.1229;

[0065] Aspergillus niger Aspergillus niger , No. IFFI.02439;

[0066] Trichoderma viride Trichodermaviride , No. CCTCCAF.93252.

[0067] (2) Slant culture:

[0068] Bacillus licheniformis slant medium (g / L): beef extract 5, peptone 10, yeast extract 5, NaCl5, agar 30, tap water, pH 7.5, cultured statically at 30°C for 24 hours;

[0069] Bacillus subtilis slant medium (g / L): peptone 10, beef extract 5, sodium chloride 5, agar 20, pH value 7.2, static culture at 30°C for 24 hours;

[0070] Aspergillus niger species use slant medium (g / L): Potato 200, glucose 20, dipotassium hydrogen phosphate 3, magnesium sulfate heptahydrate 1.5, natural pH, static culture at 0°C for 48 hours;

[0071] Trichoderma viride slant medium (g / L): sodium...

Embodiment 2

[0093] Preparation of Plant Extract Compound Enzyme Preparation

[0094] (1) The source of each strain:

[0095] Bacillus licheniformis Bacillus licheniformis , No. CGMCC1970;

[0096] Bacillus subtilis Bacillus subtilis , No. AS1.1229;

[0097] Aspergillus niger Aspergillus niger , No. IFFI.02439;

[0098] Trichoderma viride Trichodermaviride , No. CCTCCAF.93252.

[0099] (2) Slant culture:

[0100] Bacillus licheniformis slant medium (g / L): beef extract 5, peptone 10, yeast extract 5, NaCl5, agar 30, tap water, pH 7.5, static culture at 28°C for 20 hours;

[0101] Bacillus subtilis use slant medium (g / L): peptone 10, beef extract 5, sodium chloride 5, agar 20, pH value 7.2, static culture at 28°C for 20 hours;

[0102] Aspergillus niger species use slant medium (g / L): Potato 200, glucose 20, dipotassium hydrogen phosphate 3, magnesium sulfate heptahydrate 1.5, natural pH, static culture at 28°C for 20 hours;

[0103] Trichoderma viride slant medium (g / L): sodium...

Embodiment 4

[0125] Comparative Test:

[0126]

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PUM

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Abstract

The invention discloses a complex enzyme preparation for extracting effective plant components and a method for preparing the complex enzyme preparation. The complex enzyme preparation comprises components of glycanase, cellulase, pectinase, beta-glucanase and alpha-amylase. The activity of the glycanase is 3000IU / g, the activity of the cellulase is 2000IU / g, the activity of the pectinase is 20000IU / g, the activity of the beta-glucanase is 5000IU / g, and the activity of the alpha-amylase is 3000IU / g. The method for preparing the complex enzyme preparation includes symbiotically cultivating, extracting and concentrating bacillus licheniformis, bacillus subtilis, aspergillus niger and trichoderma viride according to certain proportions, then adding expanded plant protein carriers into the bacillus licheniformis, the bacillus subtilis, the aspergillus niger and the trichoderma viride; spraying mist at low temperatures to obtain the complex enzyme preparation. The complex enzyme preparation and the method have the advantages that the effective component recovery rate of complex enzymes can be increased as compared with a method for individually fermenting and producing various enzymes and then combining the various enzymes with one another, production technologies can be simplified, the production cycle can be shortened, the production cost can be lowered, the production efficiency can be greatly improved, the stability of the activity quality of final products can be guaranteed, and plant extraction complex enzyme production can be industrialized.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparation, in particular to a compound enzyme preparation for extracting effective functional components such as ginsenoside, ginkgo saponin and cucurbitaceous plant raw materials such as polysaccharide and saponin and a preparation method thereof. Background technique [0002] Plants are rich in active ingredients and are widely used in medicines, health products and cosmetics. Our country's plant resources are very rich, which is the material basis for our country's unique development and utilization advantages and the development of strategic industries. Vigorously developing the deep processing of agricultural products and improving the technological innovation capability and product competitiveness of my country's agricultural product processing industry is an important way to promote the adjustment of agricultural structure, increase employment in the secondary and tertiary industries in ru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24C12N9/42C12N9/28C12N9/88C12N9/26C12N9/18C12R1/10C12R1/125C12R1/685C12R1/885
CPCC12N9/248C12N9/18C12N9/24C12N9/2405C12N9/2414C12N9/2437C12N9/88C12Y302/01001C12Y302/01004
Inventor 揣玉多杨扬马志刚刘皓
Owner 天津科建科技发展有限公司
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