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Bacillus amyloliquefaciens strain and application thereof

A technology of amylolytic spores and bacilli, applied in the field of agricultural biology, can solve rare problems, achieve low production costs, improve raw material utilization, and reduce the loss of viable bacteria

Active Publication Date: 2015-11-18
SHANDONG ZOETICLAND BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that many microorganisms have the ability to degrade phenolic acids, including more than 20 genera such as Phanerochaete chrysosporium, Serratia marcescens, and Azospirillum lipogenicum, but Bacillus amyloliquefaciens rarely degrades phenolic acids. Substance reports

Method used

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  • Bacillus amyloliquefaciens strain and application thereof
  • Bacillus amyloliquefaciens strain and application thereof
  • Bacillus amyloliquefaciens strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening of Bacillus amyloliquefaciens ZTS-06

[0023] Bacillus amyloliquefaciens ZTS-06 was isolated from cucumber rhizosphere soil. The soil was sampled in Liangzhuang Town, Daiyue District, Tai'an City, Shandong Province, which is the soil of the protected land where cucumbers have been planted continuously for more than 3 years. The specific separation method is as follows: mix the soil sample evenly, weigh 5 g, put it into a conical flask filled with 100 mL enrichment medium and 10 glass beads, and shake and culture at 35°C and 180 rpm for 3 days. Take 1 mL of the enriched culture for 10 -1 ~10 -5 Serial concentration gradient dilution, and then take 10 -3 、10 -4 、10 -5 The three dilutions were spread on the plate containing the selection medium, and cultured upside down at 35°C for 3 days. Single colonies were picked and streaked onto plates containing selection medium, and cultured upside down at 35°C for 3 days. Pick a single colony and transf...

Embodiment 2

[0027] The identification of embodiment 2 Bacillus amyloliquefaciens ZTS-06

[0028] (1) Morphological and physiological and biochemical characteristics

[0029]The morphological characteristics of the ZTS-06 strain are: after culturing on NA medium at 35°C for 48 hours, the colony is round, white to off-white, with a moist, flat, opaque surface. The bacterium is rod-shaped, produces spores, the spores are round, not enlarged, without paraspore crystals, and the Gram stain is positive. The formula of the NA medium is: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000ml, pH7.0.

[0030] The physiological and biochemical characteristics of the ZTS-06 strain are: positive methyl red test, positive indole test, positive v-p test, positive starch hydrolysis test, positive casein hydrolysis test, positive gelatin liquefaction test, positive D-glucose test, L-Arabia The sugar test was positive, the D-xylose test was positive, the citrate utilization test was positive, the...

Embodiment 3

[0034] Example 3 Degradation Test of Bacillus amyloliquefaciens ZTS-06 to Phenylacrylic Acid, p-Hydroxybenzoic Acid and Cinnamic Acid

[0035] A medium containing phenylacrylic acid was prepared, and its formula was: 1000 mg of phenylacrylic acid, 5 g of peptone, 0.5 g of ammonium sulfate, 0.1 g of magnesium sulfate, 0.2 g of potassium chloride, 0.5 g of sodium chloride, 1000 ml of water, and pH 7.0.

[0036] Prepare a medium containing p-hydroxybenzoic acid, its formula is: p-hydroxybenzoic acid 1000mg, peptone 5g, ammonium sulfate 0.5g, magnesium sulfate 0.1g, potassium chloride 0.2g, sodium chloride 0.5g, water 1000ml, pH7. 0.

[0037] A medium containing cinnamic acid was prepared, and its formula was: 1000 mg cinnamic acid, 5 g peptone, 0.5 g ammonium sulfate, 0.1 g magnesium sulfate, 0.2 g potassium chloride, 0.5 g sodium chloride, 1000 ml water, pH 7.0.

[0038] Bacillus amyloliquefaciens ZTS-06 was transferred into the above three mediums, and cultured at 35° C. and 1...

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Abstract

The invention discloses a Bacillus amyloliquefaciens strain and application thereof. The strain is Bacillus amyloliquefaciens ZTS-06, and the collection number is CGMCC No.11165. The invention also discloses a live strain preparation containing the strain and a preparation method thereof. The live strain preparation is prepared by the following steps: carrying out amplification culture on the Bacillus amyloliquefaciens ZTS-06, inoculating in a fermentation culture medium, culturing to obtain a fermentation liquid, adding propyl gallate, beta-cyclodextrin and soluble starch, and carrying out spray drying to obtain live strain raw powder; and adding abundant soluble starch, and uniformly mixing to obtain the live strain preparation. The strain has favorable degradation effects on phenylallyl propionic acid, para-hydroxybenzoic acid, cinnamic acid and the like, and can relieve succession cropping obstacles caused by phenolic acid and other toxic substances. The living strain preparation has the advantages of short fermentation period, high fermentation liquid bacterium concentration, low live strain loss in the spray drying process, and high stability in the storage process.

Description

technical field [0001] The invention relates to a strain of bacillus amyloliquefaciens and its application, belonging to the field of agricultural biotechnology. Background technique [0002] Cucumber (CucumissativusLinn), also known as cucumber and cucumber, is a plant of Cucurbitaceae Cucumis (Cucumis) and is one of the widely cultivated vegetables. The obstacle of continuous cropping is one of the main restrictive factors affecting the yield and quality of cucumber, and with the increase of continuous planting years, the obstacle of continuous cropping becomes more and more serious. The causes of continuous cropping obstacles are complex, mainly including: soil compaction and secondary salinization, unbalanced distribution of soil nutrients, deterioration of soil micro-ecological system, aggravation of soil-borne diseases and insect pests, and plant autotoxicity. Among them, plant autotoxicity has attracted more and more attention as one of the main factors causing conti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/07
Inventor 辛寒晓范学明王泽利王维超孙中涛
Owner SHANDONG ZOETICLAND BIOLOGICAL TECH
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