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Achyranthes bidentata glycopolymer as well as preparation method and application thereof

A sugar polymer, polymer technology, applied in the preparation of refined sugar polymer, the application of the Achyranthes jaggery polymer in the prevention and/or treatment of osteoporosis, Achyranthes japonica jaggery polymer, sugar polymerization in plants It can solve the problem of osteoporosis research that has not yet been reported, and achieve the effect of remarkable effect, convenient operation and high content.

Active Publication Date: 2015-11-11
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on Achyranthes glycopolymer at home and abroad mainly focuses on its anti-tumor, anti-oxidation, immune regulation, hypoglycemic, anti-aging and other activities, and its research on the prevention and treatment of osteoporosis has not been reported yet.

Method used

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  • Achyranthes bidentata glycopolymer as well as preparation method and application thereof
  • Achyranthes bidentata glycopolymer as well as preparation method and application thereof
  • Achyranthes bidentata glycopolymer as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The extraction method of sugar polymer in the Radix Achyranthes Radix of embodiment 1

[0067] 1) Cutting: Cut 10kg of dried root of Achyranthes bidentata to 1~2cm with scissors, wash it quickly with cold water, and dry it;

[0068] 2) Water extraction: Extract the sheared Achyranthes bidentata with 10 times the volume of hot water (60-100°C) for 2 hours, collect the extract, and dry the residue.

[0069] 3) Fractional alcohol precipitation: After the extract was concentrated under reduced pressure at 60°C, ethanol was added to make the ethanol volume concentration 50%, and after standing at room temperature for 24 hours, centrifuged, collected the precipitate, weighed 323g, and it was crude sugar polymer AB1; collected the supernatant After the solution was concentrated under reduced pressure at 60°C, ethanol was added to make the volume concentration of ethanol 70%. After standing still at room temperature for 24 hours, it was centrifuged to collect the precipitate,...

Embodiment 2

[0072] The extraction method of embodiment 2 achyranthes glycopolymer ABW3-1

[0073] 1) Cutting: Cut 10kg of dried root of Achyranthes bidentata to 1~2cm with scissors, wash it quickly with cold water, and dry it;

[0074] 2) Water extraction: Extract the sheared Achyranthes bidentata with 10 times the volume of hot water (60-100°C) for 2 hours, collect the extract, and dry the residue.

[0075] 3) Alcohol precipitation: After the extract is concentrated under reduced pressure at 60°C, add ethanol to make the ethanol volume concentration 50%, let it stand for 24 hours, collect the supernatant, concentrate it under reduced pressure at 60°C, add ethanol to make the ethanol volume concentration 70% , stand still for 24 hours, repeat the same operation as above (except that the volume concentration of ethanol is 90%, other operations are the same as above), after standing at room temperature for 24 hours, centrifuge, collect the precipitate, weigh 671g, it is crude sugar polyme...

Embodiment 3

[0106] Example 3 Research on the Anti-osteoporosis Effect of Achyranthes Radix Glycopolymer

[0107] 1 Experimental method

[0108] 1.1 Experimental design and grouping: 70 3-month-old unmated female SD rats, SPF grade (provided by the Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine), were randomly divided into 7 groups according to the principle of weight equality, the number of animals in each group and the administration The methods are shown in Table 1, in which the positive control group E2 is 17β-estradiol, the model group OVX and the sham operation group SHAM are distilled water. After the operation, the drug was administered by intragastric administration every day, weighed once a week, and the corresponding dosage was adjusted according to the change of body weight, and the intragastric administration was continued for 90 days.

[0109] Table 1 Experimental design and grouping

[0110]

[0111] 1.2 Rat ovariectomy method:

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Abstract

The invention discloses a glycopolymer in achyranthes bidentata as well as a preparation method and application thereof. In the method, the achyranthes bidentata is used as a raw material, and is subjected to water-extraction alcohol precipitation and alkaline-extraction alcohol precipitation, so that polymers at all the parts of the achyranthes bidentata are obtained. The extraction method is implemented under a relatively gentle condition, and components of the glycopolymer are intactly preserved. The achyranthes bidentata glycopolymer has a remarkable effect at the aspect of anti-osteoporosis symptom; in addition, a glycopolymer pure product is obtained through separation and purification processes including Sevag method deproteinization, DEAE cellulose column chromatography, Sephacryl column chromatography and the like, and structural analysis shows that the achyranthes bidentata glycopolymer obtained through the method comprises polyose and oligose. Through the adoption of the achyranthes bidentata glycopolymer as well as the preparation method and application thereof, a basis is provided for the application of the achyranthes bidentata glycopolymer in the fields of health care products, medicines and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food, and specifically relates to a preparation method of sugar polymers in plants, more specifically to a preparation method of Achyranthes bidentata crude sugar polymers and refined sugar polymers, and also relates to the use of the Achyranthes bidentata crude sugar polymers in preventing And / or application in the treatment of osteoporosis. The sugar polymers in the present invention include polysaccharides and oligosaccharides. Background technique [0002] Osteoporosis (OP) is a disease characterized by decreased bone mineral mass, bone microstructure destruction, and decreased bone strength, resulting in increased bone fragility and susceptibility to fracture. Osteoporosis is a multiple senile disease. With the aging of the population, osteoporosis has seriously endangered the health of the elderly. Postmenopausal women are the biggest victims of the disease due to the dual effe...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/08A61K31/702A61P19/10A23L1/09
Inventor 严春艳王昌盛
Owner GUANGDONG PHARMA UNIV
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