Method for preparing dodecyl-pectin microspheres carrying sheep serum protein

A technology of dodecyl and goat serum, applied in the field of medicine, can solve problems such as degradation, and achieve the effect of good cross-linking performance

Active Publication Date: 2015-11-11
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that protein drugs are degraded by proteases in the digestive tract, propose a method for preparing dodecylated pectin-loaded goat serum albumin microspheres, and synthesize a protein drug that can effectively carry protein drugs to the colon. and released drug carrier

Method used

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  • Method for preparing dodecyl-pectin microspheres carrying sheep serum protein
  • Method for preparing dodecyl-pectin microspheres carrying sheep serum protein
  • Method for preparing dodecyl-pectin microspheres carrying sheep serum protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Take 2g of pectin and dissolve it in water to get 100mL of solution to get a 2% pectin solution, add tetrabutylammonium hydroxide solution with a mass concentration of 25% in a fume hood, and neutralize the pH of the solution to 7.0.

[0021] The pectin solution with a pH of 7.0 was freeze-dried, and after freeze-drying, 1 g of the obtained pectin powder was weighed and dissolved in 50 mL of dimethyl sulfoxide solution, 1.28 g of bromododecane was added, and the reaction was stirred at room temperature for 24 h, and Dimethylsulfoxide was removed by dialysis against ultrapure water for 7 days at room temperature.

[0022] (2) Concentrate about 20 mL of the dialysate by rotary evaporation, and prepare an ethanol solution with a molar concentration of 1 mol / L sodium chloride, and the volume fraction of ethanol is 75%. At 4°C, the concentrated sample was reacted with sodium chloride solution for 24 hours.

[0023] (3) Filtration, first wash the filter residue with etha...

Embodiment 2

[0049] (1) Dissolve 3g of pectin in water to obtain a 100mL solution, prepare a 3% pectin solution, add tetrabutylammonium hydroxide with a mass concentration of 27% to neutralize the pH of the solution to 7.0, and after freeze-drying, weigh the pectin powder to dissolve Add equimolar dodecane bromide to the dimethyl sulfoxide solution, stir at room temperature for 24 hours, and dialyze in ultrapure water for 7 days at room temperature.

[0050] (2) The concentrated solution was obtained by rotary evaporation of the dialysate, and the concentrated solution was reacted with 1 mol / L sodium chloride ethanol solution with a volume fraction of 75% at 7°C for 24 hours.

[0051] (3) Filter the solution, first wash it with ethanol with a volume fraction of 75%, and then wash it with absolute ethanol until there is no chloride ion, then wash it with anhydrous acetone, and dry it under normal temperature and pressure to obtain dodecylated pectin .

[0052] (4) Prepare an aqueous soluti...

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Abstract

The invention provides a method for preparing dodecyl-pectin microspheres carrying sheep serum protein. According to the method, pectin is modified through bromododecane, and the obtained dodecyl-pectin is used for embedding sheep serum protein to prepare microspheres carrying medicine. The prepared dodecyl-pectin has good performance of crosslinking with calcium ions and is capable of embedding the sheep serum protein effectively to form a dense structure; the sheep serum protein can be kept in the whole form in the stomach liquid, the protein medicine can be released under the colon PH, and the purpose of releasing the medicine by the colon in a fixed-point mode is achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of pectin as a drug carrier. Background technique [0002] With the rapid development of the field of biotechnology, protein drugs have quickly become a very important part of therapeutic drugs. At present, protein drugs are mainly administered by subcutaneous injection. However, this administration method has a short action time and requires multiple and frequent injections. In order to reduce the harm caused by repeated injections to the physical and mental health of patients, it is urgent to find a Non-injection route of administration instead of injection. Oral administration, which has the characteristics of reducing patient pain, convenience, and reducing cross-contamination and needle-stick injuries, has become the first choice; however, protein drugs are easily digested and degraded by proteases in the upper gastrointestinal tract, and their ...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K47/36A61K38/02
Inventor 陈军戴涛涛刘成梅梁瑞红王玲华李俶
Owner NANCHANG UNIV
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