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Sarcopyramis nepalensis wall steroidal saponin and application thereof

A technology of steroidal saponin and mulberry red, which is applied in the field of medicine, can solve the problems of low price, need for further research, and few adverse reactions of Chinese herbal medicine, and achieve the effects of less dosage, improving the body's immunity, and high development value

Inactive Publication Date: 2015-11-04
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many varieties of traditional Chinese herbal medicines, which have been widely used in the clinical treatment of chronic hepatitis B and have achieved certain curative effects, and have accumulated rich clinical experience. However, the mechanism of Chinese herbal medicines against HBV needs to be further studied, so the screening of Chinese herbal medicines against HBV remains to be done. It has great potential; and the traditional Chinese herbal medicine has few adverse reactions and low price, and has been paid more and more attention by researchers at home and abroad. Seeking natural medicines with antiviral effects is a hot spot in current research

Method used

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  • Sarcopyramis nepalensis wall steroidal saponin and application thereof
  • Sarcopyramis nepalensis wall steroidal saponin and application thereof
  • Sarcopyramis nepalensis wall steroidal saponin and application thereof

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Embodiment 1

[0017] 1. The preparation of steroidal saponins

[0018] 1.1 Extraction of steroidal saponins

[0019] Wash the whole herb of Chutouhong with distilled water, dry it at room temperature, crush it, weigh 200g of the whole herb of Chutouhong, put it in a round bottom flask (2000mL), add 1000mL of 95% ethanol, and reflux for 3h at 80°C. Filtration, repeated leaching of the filter residue twice (leaching time 2h, 2h), combined three filtrates. Ethanol was concentrated under reduced pressure into an extract. The extract was suspended in 100mL of distilled water, extracted with petroleum ether (30-60°C), chloroform, and ethyl acetate in sequence, and finally extracted with n-butanol several times until the n-butanol layer was colorless. The n-butanol layer extract was collected, concentrated under reduced pressure, and freeze-dried to obtain the thick saponin powder of chutou.

[0020] Separation and Purification of Steroidal Saponins

[0021] Crude saponins are separated by sil...

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Abstract

The present invention discloses a sarcopyramis nepalensis wall steroidal saponin and application thereof. The sarcopyramis nepalensis wall steroidal saponin is a furostanol steroidal saponin with a molecular formula of C32H50O9 and a molecular weight of 482. The sarcopyramis nepalensis wall steroidal saponin is prepared by: performing extraction on a medicinal plant sarcopyramis nepalensis wall with ethanol; then performing extraction with n-butanol; and performing isolation and purification with TLC and PHPLC. An in vitro biological activity test shows that the sarcopyramis nepalensis wall steroidal saponin has effects of inhibiting secretion of HBsAg and HBeAg and replication of HBV-DNA in HepG 2.2.2.15 cell culture, has a certain active effects on in vitro anti-Hepatitis B virus (HBV), and can be applied to preparing anti-HBV drugs.

Description

technical field [0001] The invention relates to the steroidal saponin and application thereof, and belongs to the field of medicine. Background technique [0002] Hepatitis B Virus (HBV) has been discovered for more than 40 years. In 1963, Blumberg and others found that the serum of Australian hemophiliac patients contained an antigen that could react with the serum of American hemophiliac patients, calling it the Australian antigen (ie, Hepatitis B Virus Surface Antigen, Hepatitis B Virus Surface Antigen, HBsAg ). In 1970, Dane et al. found HBV particles with a size of 42nm in the serum of hepatitis B patients, and the outer membrane component contained HBsAg. In 1986, the International Committee on Virus Nomenclature officially classified HBV as a hepadnaviridae. [0003] Hepatitis B virus (HBV) infection is a worldwide health problem with worldwide distribution. It can not only cause acute and chronic HBV infection, but also be related to 75%-90% of primary liver cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00A61P31/20A61P37/04A61P39/06
CPCC07J71/0005
Inventor 李清禄李凌峰李宇翔黄志坚谢勇平王玉林申爱丽王洪艳
Owner FUJIAN AGRI & FORESTRY UNIV
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