Extraction method of efficient active enzymes of fig latex

Abstract

The invention discloses an extraction method of efficient active enzymes of fig latex. The method comprises the steps that acetone precipitation, gel chromatography separation, anion chromatography and vacuum freeze-drying are sequentially conducted on the fig latex which is pretreated at the temperature of 4 DEG C to obtain efficient active enzyme powder. Due to the fact that pretreatment and heat exchange are conducted and the extraction process is conducted under a low-temperature condition, the influence of conditions such as long-term high temperature on reducing enzyme activity can be effectively reduced; by means of milling action of a colloid mill, fig enzymes are dissolved in material liquid as many as possible, and the subsequent extraction is facilitated. By means of continuous separation through a disc-type centrifuge and a tubular bowl centrifuge, clear fig protease material liquid is obtained. The vacuum freezing and drying technology is adopted, due to the fact that when water in materials becomes ice, the water directly sublimates to be discharged, it is guaranteed that drying is conducted on the enzymes in the low-temperature condition, and the loss of enzyme mass and reducing of the enzyme activity in the drying process are reduced.

Description

technical field [0001] The invention relates to the field of food. Specifically, it relates to a method for extracting high-efficiency active enzymes from fig milk. Background technique [0002] Fig (Ficus carica Linn.) is a flowering plant belonging to the genus Moraceae. It mainly grows in some tropical and temperate regions and is a subtropical deciduous small tree. There are currently 800 known varieties of figs, most of which are evergreen varieties, and only those that grow in temperate regions are deciduous varieties. The fruit is bulbous with a small hole in the tail, and the pollen is spread by wasps. Because of its good medicinal value, it is more and more popular. [0003] Ficin (Papain) widely exists in the stems, leaves and fruits of figs, and the content of immature fruit milk is the highest. It can be prepared by chemical methods (precipitation method) and biotechnology methods. Ficin is a plant protease, referred to as ficin, because of its good thermal s...

AI Technical Summary

Problems solved by technology

[0004] In recent years, there have been many reports on the extraction and application of ficin, but most of the crude enzymes used in the market are crude ficins. Because the extraction process of crude enzymes is simple and the production cost is low, hot air drying is generally used to obtain ficins. Vitality is only about 10000u / g; at the same time, due to the complex production process and high production cost of other refined ficinase, it is not conducive to the industrial production of refined ficinase, which also limits the application of ficinase in high-end medicine and health care products and beauty cosmetics. Industry application