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Method for refining papain

A technology of papain and papain, which is applied in the field of papain preparation, can solve the problems of unfavorable industrial production of refined papain, restriction of papain application, complex production process, etc., and achieve the reduction of enzyme activity and enzyme quality. Loss, the effect of high purity

Inactive Publication Date: 2009-11-04
徐闻县美仑生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, there have been many reports on the extraction and application of papain, but most of the crude enzymes used in the market are papain crude enzymes. Because the extraction process of crude enzymes is simple and the production cost is low, hot air drying is generally used to obtain papain. Vitality is only about 1 million u / g; at the same time, due to the complex production process and high production cost of other refined papain, it is not conducive to the industrial production of refined papain, which also limits the use of papain in medicine and health care products and beauty cosmetics and other high-end industry applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The manufacturing method of refined papain in this embodiment comprises the following steps: papaya pulp→pretreatment→grinding→heat exchange→two-stage centrifugation→microfiltration→ultrafiltration→vacuum freeze-drying→modulation, pulverization, and finished product. The specific operation is: harvest fresh papaya pulp, add 0.5% antioxidant and 0.5% heavy metal chelating agent, and fully stir for 20 minutes; pour the pretreated papaya pulp into a colloid mill and grind it fully to obtain papaya enzyme feed liquid The temperature of the papaya enzyme feed liquid is rapidly reduced to 8°C through a plate heat exchanger, and the subsequent extraction and separation process is maintained in this temperature range through a cold water circulation system; the papaya enzyme feed liquid is passed through a disc centrifuge and a tube centrifuge The continuous and continuous separation of papain enzyme to obtain clarified papaya enzyme clarified feed liquid; the clarified papain e...

Embodiment 2

[0028] The manufacturing method of refined papain in this embodiment comprises the following steps: papaya pulp→pretreatment→grinding→heat exchange→two-stage centrifugation→microfiltration→ultrafiltration→vacuum freeze-drying→modulation, pulverization, and finished product. The specific operation is: harvest fresh papaya pulp, add 1.0% antioxidant and 1.0% heavy metal chelating agent, and fully stir for 30 minutes; pour the pretreated papaya pulp into a colloid mill and grind it fully to obtain papaya enzyme feed liquid The temperature of the papaya enzyme feed liquid is rapidly reduced to 12°C through a plate heat exchanger, and the subsequent extraction and separation process is maintained in this temperature range through a cold water circulation system; the papaya enzyme feed liquid is passed through a disc centrifuge and a tube centrifuge The continuous and continuous separation of papain enzyme to obtain clarified papaya enzyme clarified feed liquid; the clarified papain ...

Embodiment 3

[0030] The manufacturing method of refined papain in this embodiment comprises the following steps: papaya pulp→pretreatment→grinding→heat exchange→two-stage centrifugation→microfiltration→ultrafiltration→vacuum freeze-drying→modulation, pulverization, and finished product. The specific operation is: harvest fresh papaya pulp, add 0.6% antioxidant and 0.6% heavy metal chelating agent, and fully stir for 25 minutes; pour the pretreated papaya pulp into a colloid mill and grind it fully to obtain papaya enzyme feed liquid The temperature of the papaya enzyme feed liquid is rapidly reduced to 10°C through a plate heat exchanger, and the subsequent extraction and separation process is maintained in this temperature range through a cold water circulation system; the papaya enzyme feed liquid is passed through a disc centrifuge and a tube centrifuge The continuous and continuous separation of papain enzyme to obtain clarified papaya enzyme clarified feed liquid; the clarified papain ...

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Abstract

The invention discloses a method for refining papain, which comprises the following steps: preparation of pawpaw puree; pretreatment; milling; heat exchange; two stages of centrifugation; micro-filtration; hyperfiltration; freeze drying in vacuum; pulverization and modulation; and preparation of a finished product. The extraction method is characterized in that: (1) by carrying out the pretreatment and the heat exchange and keeping an extraction process being carried out under a low-temperature condition, the influence on activity reduction of the papain under the conditions of high temperature for a long time, and the like can be effectively reduced; (2) by milling, the papain can be sufficiently dissolved in material liquid so as to be beneficial to improving the extraction and recovery ratio of the papain; (3) the papain extracted by the method has high purity and activity (the highest activity can reach 3,000,000u / g) and good thermal stability; indexes such as appearance, rationalization, microbial limit, and the like can reach or exceed relevant food and medicine standards at home and abroad; by modulation, products in different specifications can be produced and widely applied to the industries and the fields of medicinal health products, foods, cosmetics, chemical leather manufacture, and the like; and (4) the method has simple process, low production cost, high yield and industrial production.

Description

technical field [0001] The invention relates to a method for preparing papain, in particular to extracting and refining papain by using papaya pulp as a raw material and combining methods such as grinding, two-stage separation, ultrafiltration, vacuum freeze-drying, etc., belonging to the field of biotechnology. Background technique [0002] Papain (Papain) widely exists in papaya stems, leaves and fruits, and the content of immature fruit milk is the highest. It can be prepared by chemical methods (precipitation method) and biotechnology methods. Papain is a plant protease, referred to as papain, because of its good thermal stability, it has a very strong ability to decompose animal and plant proteins, lipids, amides, etc., and can be widely used in medicine and health products, food, cosmetics, and chemical leather. and other industries and fields. [0003] In recent years, there have been many reports on the extraction and application of papain, but most of the crude enz...

Claims

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Application Information

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IPC IPC(8): C12N9/50
Inventor 龙晓鹏林森黄忠华
Owner 徐闻县美仑生物制品有限公司
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