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Method for producing anti-human C-reaction protein antiserum for sheep

A technology of reactive protein and antiserum, applied in the direction of anti-animal/human immunoglobulin, immunoglobulin from serum, etc., can solve the problems of low level, high antigen dosage, slow antibody production speed, etc., to achieve simple process, antigenic The effect of small dosage and high antibody level

Inactive Publication Date: 2015-10-21
海奥斯生物科技镇江有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current production methods can produce a certain amount of antibodies, the antibody production speed is slow, the level is low, and the amount of antigen is high

Method used

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  • Method for producing anti-human C-reaction protein antiserum for sheep

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Choose 5 adult healthy goats and proceed with the following steps:

[0026] (1) Antigen preparation: select human C-reactive protein antigen and emulsify it with Freund's adjuvant before injection to obtain an antigen emulsion;

[0027] (2) The first injection: Take 2.5 mg of human C-reactive protein antigen, dilute to 5 ml with PBS or saline, and then add 5 ml of complete Freund’s adjuvant to make an antigen emulsion. Use this antigen emulsion to inject the sheep. Multi-point subcutaneous injection for plantar, groin, axilla, neck, back, 1-2 points for each part, 10-16 points in total, the injection dose of each sheep is 2ml containing 0.5mg antigen, and the dose of each injection is evenly distributed ;

[0028] (3) Second injection: Take the second injection 20 days after the first injection, take 5.0 mg of human C-reactive protein antigen, dilute to 5 ml with PBS or saline, and add 5 ml of incomplete Freund’s adjuvant to make it Antigen emulsion, use this antigen emulsio...

Embodiment 2

[0034] Choose 5 adult healthy sheep and proceed with the following steps:

[0035] (1) Antigen preparation: select human C-reactive protein antigen and emulsify it with Freund's adjuvant before injection to obtain an antigen emulsion;

[0036] (2) The first injection: Take 2.5 mg of human C-reactive protein antigen, dilute to 5 ml with PBS or saline, and then add 5 ml of complete Freund’s adjuvant to make an antigen emulsion. Use this antigen emulsion to inject the sheep. Multi-point injections for the sole, groin, axilla, neck, and back under the skin, 1-2 points for each site, 10-16 points in total, the injection dose of each sheep is 2ml containing 0.5mg antigen, and the dose of each injection is evenly distributed ;

[0037] (3) Second injection: Take the second injection 20 days after the first injection, take 5.0mg of human C-reactive protein antigen, dilute to 5ml with PBS or saline, and add 5ml of incomplete Freund’s adjuvant to make it Antigen emulsion, use this antigen em...

Embodiment 3

[0043] Choose 5 adult healthy goats and proceed with the following steps:

[0044] (1) Antigen preparation: select human C-reactive protein antigen and emulsify it with Freund's adjuvant before injection to obtain an antigen emulsion;

[0045] (2) The first injection: Take 2.5 mg of human C-reactive protein antigen, dilute to 5 ml with PBS or saline, and then add 5 ml of complete Freund’s adjuvant to make an antigen emulsion. Use this antigen emulsion to inject the sheep. Multi-point injections for the sole, groin, axilla, neck, and back under the skin, 1-2 points for each site, 10-16 points in total, the injection dose of each sheep is 2ml containing 0.5mg antigen, and the dose of each injection is evenly distributed ;

[0046] (3) Second injection: Take the second injection 20 days after the first injection, take 5.0 mg of human C-reactive protein antigen, dilute to 5 ml with PBS or saline, and add 5 ml of incomplete Freund’s adjuvant to make it Antigen emulsion, use this antigen...

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Abstract

The invention provides a method for producing anti-human C-reaction protein antiserum for sheep. The method comprises the following steps of: (1) preparing an antigen; (2) performing injection for the first time: using the antigen emulsion to inject the sheep, wherein injection parts are soles of feet, inguinal regions, armpits, necks, backs and subcutaneous multipoint injection; (3) performing injection for the second time; (4) performing injection for the third time; (5) performing injection for the fourth time; (6) performing injection for the fifth time; and (7) detecting serum titer: drawing blood from the vein of the sheep after 8-10 days from the injection each time, separating the serum and detecting dual-extending titer. Compared with the prior art, the method for producing the anti-human C-reaction protein antiserum for the sheep, disclosed by the invention, is low in cost, and simple in process; the method not only can stably produce the antibodies, but also is high in antibody producing speed; the antibody level is high, and the dosage of the antigen is small.

Description

Technical field [0001] The invention relates to a method for producing goat anti-human C-reactive protein antiserum, and belongs to the technical field of antiserum preparation. Background technique [0002] C-reactive protein (CRP) refers to some proteins that rise sharply in plasma when the body is infected or tissue damage. CRP can activate complement and strengthen the phagocytic function of phagocytes, thereby eliminating pathogenic microorganisms and damage that invade the body , Necrotic, and apoptotic tissue cells play an important protective role in the body's natural immune process. Its molecular weight is 105KD. [0003] CRP is the first protein considered to be an acute phase response protein, and its content is very small under normal conditions. Its plasma concentration increases sharply during acute trauma and infection. CRP is the most commonly used clinical acute phase response index. The concentration of CRP in plasma increases rapidly and significantly during a...

Claims

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Application Information

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IPC IPC(8): C07K16/18C07K16/06
Inventor 张传明袁金城徐志伟
Owner 海奥斯生物科技镇江有限公司
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