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A method for isolating a large amount of natural sweetener trilobatin

A natural sweetener, trilobatin technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of low separation efficiency and failure to realize large-scale preparation and separation, and achieve large-scale preparation , shorten the separation time, and the effect of simple separation method

Active Publication Date: 2017-12-15
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for the separation of natural sweetener trilobatin in large quantities, aiming to solve the problems of low separation efficiency of existing natural sweeteners and the failure to achieve large-scale preparation and separation

Method used

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  • A method for isolating a large amount of natural sweetener trilobatin
  • A method for isolating a large amount of natural sweetener trilobatin
  • A method for isolating a large amount of natural sweetener trilobatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Preparation of Duosuike sweet tea extract:

[0039] Take the dried Duosuike sweet tea leaves, and the samples are pulverized by a pulverizer. Weigh 500 g of Duosuike sweet tea powder, add 20 times the weight of ethanol solution with a volume fraction of 50%, reflux for extraction for 3 hours, and filter; repeat the above steps for the filter residue, and then extract twice, combine the filtrates, and concentrate under reduced pressure to extract Shape, Duosuike sweet tea extract 45.6g.

[0040] (2) Further purification of Duosuike sweet tea extract:

[0041] After taking 20 g of the sweet tea extract obtained in step (1) and adding an appropriate amount of water to suspend it, put it into a separatory funnel, add ethyl acetate to extract 500 mL, extract 3 times, collect the ethyl acetate extract, and rotate Concentrate and dry under reduced pressure by evaporation to obtain 8.3 g of ethyl acetate extract.

[0042] (3) trilobatin in the crude ethyl acetate isolate...

Embodiment 2

[0050] (1) Preparation of Duosuike sweet tea extract:

[0051] Take 200 g of Duosuike sweet tea powder pulverized by a pulverizer, add 50 times the weight of the ethanol solution with a volume fraction of 70%, reflux for extraction for 2.5 hours, and filter; repeat the above steps for the filter residue, and then extract twice, combine the filtrate, and depressurize Concentrate to the extract form, Duosuike sweet tea extract 21.4g.

[0052] (2) Further purification of Duosuike sweet tea extract:

[0053] After taking 10 g of the sweet tea extract obtained in step (1) and adding an appropriate amount of water to suspend it, put it into a separatory funnel, add ethyl acetate to extract 400 mL at the same time, extract 3 times, collect the ethyl acetate extract, and rotate Evaporate and concentrate to dry under reduced pressure to obtain 4.2 g of ethyl acetate extract.

[0054] (3) high-speed countercurrent chromatographic separation of trilobatin in the ethyl acetate extract:

...

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Abstract

The invention provides a method for isolating a large amount of natural sweetener trilobatin, by adding ethanol with a volume fraction of 30-90% to the pulverized trilobatin raw material at a solid-to-liquid mass ratio of 1:10-50, and heating to reflux Separating, filtering the separating liquid, separating the filter residue and filtrate, concentrating the filtrate under reduced pressure until it has no alcohol smell, and obtaining the isolate; dissolving the isolate in water, and extracting with ethyl acetate, and concentrating the extract under reduced pressure to obtain ethyl acetate extract; The ethyl acetate extract was separated by high-speed countercurrent chromatography to obtain a natural sweetener. The separation method of the invention is simple and quick, the separation period is short, the monomer preparation amount is large, and the monomer has various pharmacological activities.

Description

technical field [0001] The invention belongs to the technical field of monomer separation, and in particular relates to a method for separating a large amount of natural sweetener trilobatin. Background technique [0002] Sweeteners refer to food additives that impart sweetness to food or feed, improve food quality, and meet people's needs for food. Sweeteners generally have the advantages of high safety, good taste, high stability, and good water solubility. In order to improve the mouthfeel of food and medicine, many items must be added with appropriate amount of sweetener. Sucrose is the most commonly used sweetener, but sucrose is the carbohydrate with the highest caloric value. Excessive intake can cause diseases such as obesity, arteriosclerosis, high blood pressure, diabetes, and dental caries. Therefore, actively looking for and developing new sweetener resources has become an important research direction for scientists. [0003] With the continuous deepening of r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08
Inventor 孙印石张玉伟
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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