Extraction method of mycelial fungus genome
A technology of filamentous fungus and extraction method, which is applied in the field of genome extraction of Penicillium chrysogenum, can solve the problems of corrosiveness, toxicity, and lengthy extraction process of organic reagents, and achieve the goal of reducing damage, simplifying the operation process, and avoiding the use Effect
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[0033] This example relates to the extraction method of filamentous fungal genome.
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[0035] This embodiment relates to a method for extracting genomes of filamentous fungi.
Embodiment 111
[0037] This embodiment relates to a method for extracting the genome of Penicillium chrysogenum, comprising the following extraction steps:
[0038] a. Obtain the mycelia of Penicillium chrysogenum: inoculate the rice spores of Penicillium chrysogenum on solid medium for culture, pick the clone to obtain the mycelium of Penicillium chrysogenum; 3 5g / L Penicillium chrysogenum liquid growth medium, shake culture at 26°C for 48h, centrifuge at 10000rpm for 2min to obtain the mycelia of Penicillium chrysogenum;
[0039] b. Add enzyme solution: add 60 μL of helicase solution containing helicase concentration of 10 mg / mL and 40 μL of cellulase solution containing cellulase concentration of 10000 U / mL to the mycelia of Penicillium chrysogenum in step a, totaling 100 μL of mixed enzyme solution , to obtain the Penicillium chrysogenum mycelia liquid; the mixed enzyme liquid is prepared by dissolving commercially available helicase and cellulase in the penetrant respectively, the helica...
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