Compositions, formulations, applications thereof, methods of preparation and methods of use
A composition and preparation technology, applied in the field of compositions, can solve the problems of poor stability, high injection frequency, and insignificant improvement of melanin.
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[0045] The application also provides a preparation method of the preparation, comprising the following steps:
[0046] mixing hyaluronidase with a solvent to obtain a first preparation;
[0047] The second preparation is obtained by mixing deer antler essence, vitamin C, persimmon leaf extract, autologous platelet-rich plasma, autologous platelet-rich plasma activator and hyaluronic acid.
[0048] The preparation methods of the autologous platelet-rich plasma (PRP) and the autologous platelet-rich plasma (HT) have been described in detail, and will not be repeated here.
[0049] The preparations provided by this application include the first preparation and the second preparation, wherein the first preparation contains hyaluronidase, which promotes the hydrolysis of hyaluronic acid in the extracellular matrix, reduces the hindrance of the extracellular matrix to the injection of nutrients, and improves the elasticity of the dermal tissue. Permeability, thereby improving the p...
Embodiment 1
[0051] The preparation of embodiment 1 autologous platelet-rich plasma (PRP)
[0052] 1) Collect 10-20ml of autologous peripheral blood;
[0053] 2) Centrifuge the blood for the first time (400G, 5min);
[0054] 3) The centrifuge tube can be roughly divided into 3 layers from top to bottom: supernatant, platelet-rich layer and red blood cell layer, extract the upper yellow supernatant and the 2mm red blood cell layer at the junction, and perform a second centrifugation (400G, 10min) ;
[0055] 4) After extracting all the supernatant, divide it into two centrifuge tubes, extract 4ml from one tube and keep it as the subsequent preparation of autologous PRP activator, and perform the third centrifugation (1200G, 5min) on the remaining one to extract the upper plasma And leave about 1-2ml of PRP at the bottom of the tube.
Embodiment 2
[0057] 1) Let the 2-4ml of the above-mentioned reserved supernatant stand for 30 minutes and then centrifuge (800G, 15 minutes);
[0058] 2) Then remove the upper layer of plasma, keep the bottom 0.75 ~ 1.5ml of plasma, and then mix it with 5% ~ 10% CaCl at a ratio of 3:1 2 Mixed (HT: CaCl 2 =3:1), to obtain 1-2ml of PRP activator (HT) modulated by homemade thrombin.
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