Method for measuring inhibition zone of yellow rice wine by filter paper dispersion method

A technology of filter paper and diffusion method, which is applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problem that the systematic research on rice wine process has not been reported in detail.

Inactive Publication Date: 2015-09-16
SHAOXING UNIVERSITY
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Problems solved by technology

[0004] There are many related reports on the production of rice wine, but th...

Method used

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  • Method for measuring inhibition zone of yellow rice wine by filter paper dispersion method

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preparation example Construction

[0022] (1) Preparation of bacterial solution: Inoculate Escherichia coli and Staphylococcus aureus strains on the slant of beef extract peptone medium, incubate at 37°C for 24 hours, pick bacteria from the slant with an inoculation loop into 100mL sterile water to make a bacterial solution, and use The tampon is ready for use;

[0023] (2) Preparation and treatment of filter paper sheets: use a puncher to punch several circular filter paper sheets with a diameter of 5.5 mm, dry heat sterilization at 120°C for 2 hours, and then use tweezers to pick up the sterilized filter paper sheets and soak them in Prepare in different concentrations of liquid medicine and wine samples; use filter paper soaked in sterile water as blank;

[0024] (3) Drug dilution: Inhale 20 μL of the drug solution as a standard. If the paper is thin, the drug stock solution needs to be adjusted; prepare the drug stock solution according to the different concentrations of various antibacterial drugs in the p...

Embodiment 1

[0033] Using 4% alcohol and sterile water as controls, the inhibitory effects of different concentrations of medicinal solutions and wine samples added with functional Chinese medicinal materials on Staphylococcus aureus and Escherichia coli were determined. The size of the inhibition zone is shown in Table 1 below.

[0034]

[0035] The data in the table is the average value of the diameter of the bacteriostatic zone, in mm, the larger the diameter of the bacteriostatic zone, the better the bacteriostatic effect, "—" means no bacteriostatic zone.

[0036] It can be seen from the above table 1 that different concentrations of traditional Chinese medicine extracts have certain inhibitory effects on Staphylococcus aureus and Escherichia coli, but the inhibitory effect on Staphylococcus aureus is stronger, and its inhibition zone is larger than that of Escherichia coli ; The wine samples made with functional Chinese medicinal materials had stronger inhibitory effects on Staphy...

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Abstract

The invention relates to a method for measuring the inhibition zone of yellow rice wine by a filter paper dispersion method, which comprises the following steps of: (1) bacterial solution preparation: inoculating escherichia coli and staphylococcus aureus strains on the inclined plane of a beef extract-peptone culture medium, picking bacteria from the inclined plane to 100 mL of sterile water by an inoculating loop to prepare the bacterial solution, and plugging by tampon for later use; (2) preparation and treatment of filter paper: punching round filter paper by a puncher, performing dry heat sterilization for later use, clamping the sterilized filter paper by forceps and soaking the filter paper into medicine liquid and wine samples with different concentrations for later use; and (3) inoculation and cultivation: preparing a flat plate by the sterilized beef extract-peptone culture medium under the aseptic condition, adding bacteria for testing after cooling and solidification, coating uniformly by an aseptic coating device, clamping the filter paper, which is subjected to extraction treatment, by aseptic forceps, and adhering the filter paper to a bacterium-containing plate. By the method, the inhibition effect of mildew and bacteria can be controlled, so that a theoretical basis and a technical support are provided for production, research and development of the functional yellow rice wine.

Description

technical field [0001] The invention relates to a method for measuring the bacteriostatic zone of rice wine by a filter paper sheet diffusion method, and belongs to the technical field of rice wine brewing. Background technique [0002] Yellow rice wine is one of the oldest wines in the world, and it is also a special product of my country, enjoying the reputation of "national wine". Because of its moderate alcohol content, rich aroma, unique taste, rich nutrition and therapeutic value, it is very popular among consumers. [0003] The composition of yellow rice wine is quite complex, and its wine body is an unstable colloid. Changes in factors such as temperature, light, and pH value during storage will destroy the balance of its colloid to a certain extent, so that some substances in it will be precipitated and formed. precipitation. Although these precipitations are harmless to the human body, they seriously affect the appearance quality of yellow rice wine. It is immin...

Claims

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Application Information

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IPC IPC(8): C12Q1/18
Inventor 鲁志康张苏敏
Owner SHAOXING UNIVERSITY
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