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Method for producing protein feed material by enzymolysis and fermentation of guar meal

A technology of guar soybean meal and protein feed, which is applied in the field of guar soybean meal enzymolysis and fermentation to produce protein feed raw materials, can solve the problems of reducing the digestibility of nutrients, increasing the viscosity of chyme in the digestive tract, etc., to reduce the pollution of miscellaneous bacteria, The effect of shortening the fermentation cycle and high biotransformation rate

Inactive Publication Date: 2015-09-16
河南双成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Galactomannan is highly water-soluble and viscous, which can significantly increase the viscosity of chyme in the digestive tract, thus reducing the digestibility of various nutrients in the feed
In addition, guar soybean meal, like most other cakes, contains anti-nutritional factors such as trypsin inhibitors, antigenic proteins, uric acid, lectin, and non-starch polysaccharides, so it cannot be directly used as protein feed

Method used

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  • Method for producing protein feed material by enzymolysis and fermentation of guar meal
  • Method for producing protein feed material by enzymolysis and fermentation of guar meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The method for producing protein feed material by enzymatic hydrolysis and fermentation of guar soybean meal of the present invention comprises the following steps:

[0052] The first step, liquid fermentation

[0053] The specific preparation method of Candida utilis fermented liquid, Bacillus natto fermented liquid, Lactobacillus plantarum fermented liquid is the same as described in the specific embodiment, and it is standby to make mixed bacterial liquid by the ratio of 1:1:1;

[0054] The second step, solid enzymatic hydrolysis and fermentation

[0055] a. Put the raw material of guar soybean meal into the explosion tank, the filling coefficient is 60~80%, and the saturated steam at 215°C is introduced after the explosion tank body is sealed. steam explosion;

[0056] b. Mix the gas-exploded material, the mixed bacterial solution prepared in the first step, and molasses in a mixer at a weight ratio of 1:1.2:0.08, and add neutral protease 75 U / g raw materials, ...

Embodiment 2

[0063] The method for producing protein feed material by enzymatic hydrolysis and fermentation of guar soybean meal of the present invention comprises the following steps:

[0064] The first step, liquid fermentation

[0065] The specific preparation method of Candida utilis fermented liquid, Bacillus natto fermented liquid, Lactobacillus plantarum fermented liquid is the same as described in the specific embodiment, and it is standby to make mixed bacterial liquid by the ratio of 2:1:1;

[0066] The second step, solid enzymatic hydrolysis and fermentation

[0067] a. Put the raw material of guar soybean meal into the explosion cylinder, the filling coefficient is 60~80%. After the explosion cylinder is sealed, the saturated steam at 185°C is introduced. When the pressure of the explosion cylinder reaches 0.5Mpa, keep it for 10 minutes, and immediately open the pneumatic valve to carry out steam explosion;

[0068] b. Mix the gas-exploded material, the mixed bacterial liq...

Embodiment 3

[0075] The method for producing protein feed material by enzymatic hydrolysis and fermentation of guar soybean meal of the present invention comprises the following steps:

[0076] The first step, liquid fermentation

[0077] The specific preparation method of Candida utilis fermented liquid, Bacillus natto fermented liquid, Lactobacillus plantarum fermented liquid is the same as described in the specific embodiment, and it is standby to make mixed bacterial liquid by the ratio of 2:2:1;

[0078] The second step, solid enzymatic hydrolysis and fermentation

[0079] a. Put the raw material of guar soybean meal into the explosion cylinder, the filling coefficient is 60~80%, and the explosion cylinder tank is sealed and then filled with saturated steam at 250°C. When the pressure of the explosion cylinder reaches 2.0Mpa, keep it for 0.5min, and immediately open the pneumatic valve conduct steam explosion;

[0080] b. Mix the material after gas explosion, the mixed bacterial so...

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Abstract

The invention discloses a method for producing protein feed material by enzymolysis and fermentation of guar meal, comprising the steps of: liquid culturing candida utilis fermentation liquor, bacillus natto fermentation liquor and lactobacillus plantarum fermentation liquor; preparing a mixed bacterium solution according to the proportion; putting guar meal into an explosion tank, and performing steam explosion under certain conditions; mixing the materials after steam explosion with the mixed bacterium solution and molasses according to the proportion, and meanwhile adding enzymatic preparations to prepare mixed materials; sending the mixed materials into a special modular solid state biological material continuous fermentation device for performing enzyme solution and fermentation, drying the fermented materials and thereby obtaining the protein feed material. According to the method, the modular automatic fermentation device is adopted, and after being mixed with the fermentation bacterium solution and the enzymatic preparations, the materials after explosion is put into the automatic fermentation device for performing enzyme solution and biological fermentation, so that the requirements of automatically controlling temperature, humidity and ventilation quantity are achieved, the biotransformation efficiency is high, the fermentation period is short, the loss is little, the costs are low, and the requirement of mechanized and large-scale production can be achieved.

Description

technical field [0001] The invention relates to waste utilization of guar soybean meal, in particular to a method for producing protein feed raw material by enzymatic hydrolysis and fermentation of guar soybean meal. Background technique [0002] Guar meal is a by-product of the extraction of guar gum from guar beans. It contains a high level of protein (up to 48.9% to 55.16%), and the amino acid is relatively balanced. In today's shortage of protein feed, guar soybean meal can be used as a new type of protein feed for livestock and poultry in livestock and poultry breeding. However, since guar soybean meal contains residual guar gum components, it is mainly galactomannan. Galactomannan is highly water-soluble and viscous, which can significantly increase the viscosity of chyme in the digestive tract, thus reducing the digestibility of various nutrients in the feed. In addition, guar soybean meal, like most other cakes, contains anti-nutritional factors such as trypsin inh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/00C12P21/06C12R1/07C12R1/72C12R1/25
Inventor 曹胜炎李伟张军峰
Owner 河南双成生物科技有限公司
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