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Method for producing acetone, butanol and ethyl alcohol through mixed-bacterial fermented cassava

A technology of mixed bacterial fermentation and Clostridium acetobutylicum, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of high energy consumption, high amylase and saccharification enzymes, and low total solvent yield. and other problems, to achieve the effect of high ethanol yield, avoid high cost and improve yield

Inactive Publication Date: 2015-09-09
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are following problems and deficiencies in the current technology: 1. The saccharification process of raw materials in the cassava ethanol fermentation process needs to add amylase and glucoamylase, which are expensive and reduce the price competitiveness of the product; 2. The cassava ethanol fermentation process Liquefaction, saccharification and fermentation are carried out separately, and the whole process consumes a lot of energy
3. The yield of total solvent (acetone, butanol and ethanol) in the cassava butanol fermentation process is low, only 0.30-0.35g / g glucose

Method used

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  • Method for producing acetone, butanol and ethyl alcohol through mixed-bacterial fermented cassava
  • Method for producing acetone, butanol and ethyl alcohol through mixed-bacterial fermented cassava

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Embodiment 1

[0029] 1. Preparation of seed medium:

[0030] Preparation of Clostridium acetobutylicum seed culture medium: crush corn, pass through a 40-mesh sieve, add water to adjust the slurry to 5% (m / V), cook at 100°C for 20 minutes, and divide the corn mash into 20mL test tubes, 10mL test tubes, 121 Sterilize at ℃ for 120 min to obtain the seed culture medium of Clostridium acetobutylicum.

[0031] Saccharomyces cerevisiae seed medium preparation: glucose 20g / L, peptone 20g / L, yeast powder 10g / L, sterilized at 115°C for 30min to obtain Saccharomyces cerevisiae seed medium.

[0032] 2. Preparation of cassava fermentation medium: crush cassava dry, pass through a 40-mesh sieve, add water to adjust slurry to cassava content 75g / L, bran 5g / L, sterilize at 121°C for 120min to obtain cassava fermentation medium.

[0033] 3. Fermentation process

[0034] (1) Clostridium acetobutylicum seed culture: heat-shock the spore suspension of Clostridium acetobutylicum at 100°C for 90s, cool it dow...

Embodiment 2

[0044] 1, preparation seed culture medium: with embodiment 1.

[0045] 2, preparation of cassava fermentation medium: with embodiment 1.

[0046] 3. Fermentation process:

[0047] (1) Clostridium acetobutylicum seed culture: with embodiment 1.

[0048] (2) Saccharomyces cerevisiae seed culture: with embodiment 1.

[0049] (3) Inoculate matured Clostridium acetobutylicum seed solution into 200 mL of cassava fermentation medium for anaerobic fermentation. The culture temperature is 37° C., the inoculum size is 5%, and the fermentation time is 24 hours. 24 hours after the inoculation of Clostridium acetobutylicum, inoculate the mature Saccharomyces cerevisiae seed liquid into the cassava fermentation medium of Clostridium acetobutylicum, mix evenly, and carry out mixed-bacteria fermentation, the inoculum size is 5%, and the fermentation temperature is 30°C. The fermentation time is 108h.

[0050] (4) Determination of acetone, butanol and ethanol content in the fermentation brot...

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Abstract

The invention discloses a method for producing acetone, butanol and ethyl alcohol through mixed-bacterial fermented cassava. The method includes: taking the cassava as a carbon source, adding nitrogen and phosphorus source and preparing a cassava fermentation medium via size mixing and sterilizing; generating amylase and saccharifying enzyme by the aid of clostridium acetobutylicum, and saccharifying the cassava fermentation medium to obtain saccharomyces cerevisiae fermentation sugar so as to avoid high cost brought by commercial amylase and saccharifying enzyme; meanwhile, generating the acetone, the butanol and the ethyl alcohol through the saccharomyces cerevisiae fermented sugar saccharified by the saccharomyces cerevisiae fermented cassava to realize clostridium acetobutylicum and the saccharomyces cerevisiae cooperated mixed fermentation, wherein yield of solvent is obviously increased. The acetone, the butanol and the ethyl alcohol are produced by taking the cassava as raw materials by means of fermentation, non-staple crop fermentation production of the acetone, the butanol and the ethyl alcohol is realized, and great significance is achieved for production research of non-staple crop liquid biofuels.

Description

Technical field: [0001] The invention relates to a method for producing acetone, butanol and ethanol, in particular to a method for producing acetone, butanol and ethanol by fermenting cassava with mixed bacteria. Background technique: [0002] The existing process of producing ethanol by fermenting cassava as raw material is through steaming, gelatinization, liquefaction, saccharification and fermentation of cassava raw material, and distilling and separating the fermented mash to obtain ethanol. It is steamed to make it gelatinized, α-amylase is added for liquefaction, and then glucoamylase is added to hydrolyze the starch into fermentable sugar, nitrogen and phosphorus sources are added and sterilized, and then mature Saccharomyces cerevisiae seeds are inoculated for fermentation. Or the saccharification process and the fermentation process are carried out simultaneously, and after the fermentation, the fermented mash is distilled to obtain ethanol. [0003] The existing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/28C12P7/16C12P7/06C12R1/145C12R1/865
CPCY02E50/10
Inventor 陈新德齐高相熊莲林晓清陈雪芳黄超程镜蓉
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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