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Extraction method of Sparassis crispa fermentation product extract for cigarettes as well as application of extract

An extraction method and hydrangea technology are applied in the field of preparation of flavors and fragrances for tobacco to achieve the effect of natural fragrance, low cost and simple process

Inactive Publication Date: 2015-09-09
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the use of the fermented product extract of Hydrangea submerged fermentation for tobacco additives

Method used

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  • Extraction method of Sparassis crispa fermentation product extract for cigarettes as well as application of extract
  • Extraction method of Sparassis crispa fermentation product extract for cigarettes as well as application of extract
  • Extraction method of Sparassis crispa fermentation product extract for cigarettes as well as application of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Cut the small tissue fragments from the stipe of the Hydrangea sporocarp under aseptic conditions, inoculate them on the separation agar plate, and culture them statically at 28°C for 7 days. 10 pieces of agar with hyphae were picked with an inoculation hook under the conditions, inoculated into the seed medium, and cultivated for 5 days at a temperature of 28° C. and a shaker speed of 150 r / min to obtain Hydrangea inoculum. Inoculate the hydrangea inoculum into the fermentation medium at a volume ratio of 10%, at a temperature of 30°C, with an aeration rate of 1.0dm 3 / min, under the condition of rotating speed 150r / min, cultivated for 7 days, and carried out submerged fermentation to obtain hydrangea fermentation mixture;

[0017] (2) Mix hydrangea fermentation product and ethanol with a mass concentration of 95% in a volume ratio of 2:1 and add it into a distillation device evenly, carry out distillation and reflux extraction at a temperature of 90° C. for 1 hour...

Embodiment 2

[0019] (1) Cut the hydrangea sporocarp from the stipe under aseptic conditions to cut small tissue fragments, inoculate them on the separation agar plate, and culture them statically at 30°C for 5 days. Pick 15 pieces of agar with mycelium with an inoculation hook under the conditions, inoculate them into the seed medium, and cultivate them for 6 days at a temperature of 26° C. and a shaker speed of 150 r / min to obtain hydrangea inoculum. Inoculate the hydrangea inoculum into the fermentation medium at a volume ratio of 15%, at a temperature of 28°C, and an aeration rate of 1.5dm 3 / min, under the condition of rotating speed 120r / min, cultivated for 5 days, and carried out submerged fermentation to obtain hydrangea fermentation mixture;

[0020] (2) Mix hydrangea fermentation product and ethanol with a mass concentration of 90% in a volume ratio of 0.5:1 and add it into a distillation device evenly, carry out distillation and reflux extraction at a temperature of 88° C. for 2 ...

Embodiment 3

[0022] (1) Cut the hydrangea sporocarp from the stipe part under aseptic conditions to inoculate small pieces of tissue fragments on the separation agar plate, and culture it at a temperature of 29°C for 6 days. Under the conditions, 20 pieces of agar with hyphae were picked with an inoculation hook, inoculated into the seed medium, and cultured for 7 days at a temperature of 27° C. and a shaker speed of 150 r / min to obtain Hydrangea inoculum. Inoculate the hydrangea inoculum to the fermentation medium at a volume ratio of 5%, at a temperature of 25°C, and an aeration rate of 0.5dm 3 / min, under the condition of rotating speed 170r / min, cultivated for 3 days, and carried out submerged fermentation to obtain hydrangea fermentation mixture;

[0023] (2) Mix hydrangea fermentation product and ethanol with a mass concentration of 92% in a volume ratio of 1:1 and add it into a distillation device evenly, carry out distillation and reflux extraction at a temperature of 85° C. for 3 ...

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Abstract

The invention provides an extraction method of a Sparassis crispa fermentation product extract for cigarettes. The method comprises steps as follows: (1) firstly, Sparassis crispa fruiting bodies are inoculated, Sparassis crispa inoculum is obtained, then a fermentation medium is inoculated with the Sparassis crispa inoculum, submerged fermentation is performed, and a Sparassis crispa fermentation mixture is obtained; (2) reflux extraction is performed on the Sparassis crispa fermentation mixture obtained in Step (1) with ethyl alcohol with the mass concentration ranging from 90% to 95%, then filtration is performed, a filtrate is concentrated, and the Sparassis crispa fermentation product extract for the cigarettes is obtained. A submerged fermentation product of Sparassis crispa is extracted to be applied to essence and flavors for the cigarettes, the extract is natural, non-toxic and high in controllability and is not affected by factors such as seasons, weather, diseases and the like, the yield is stable, the production cycle is short, and the cost is low. Meanwhile, the prepared essence and flavors for the cigarettes are more natural and lasting in aroma, has better coordination performance, and can coordinate and cooperate with other essence and flavors for the cigarettes for aroma enhancement. The technology is simple, the cost is low, and the method has the very high practicability.

Description

technical field [0001] The invention relates to the field of preparation of flavors and fragrances for tobacco, in particular to a method for extracting hydrangea fermented tobacco extract and its application. Background technique [0002] Hydrangea is Basidiomycetes, Hydrangeaceae fungus, also known as hydrangea mushroom, which grows at an altitude of 2500-3700 and is mainly distributed in Heilongjiang, Jilin, Hebei, Yunnan, Shaanxi, Sichuan, Gansu, Tibet, Guangdong, Fujian and other places. Hydrangea per hectogram contains 15.58% protein, 7.95% fat, 58% polymer polysaccharide, 12% mannitol, 5% carbohydrate, multivitamins and amino acids and other nutrients. It is a high-grade tonic with strong Essence, kidney tonifying, fluid production, lung moistening, refreshing and brain strengthening, anti-fatigue functions. It is rich in more than 60% polymer hydrangea glucan. This special ingredient is the first choice drug for treating cancer. In addition to treating various cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A24B15/20C12R1/645
Inventor 郭国宁刘祥浩岳海波
Owner HUBEI CHINA TOBACCO IND
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