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Triple-negative breast cancer cisplatin drug-resistance cell line, preparation method and uses thereof

A technology of triple-negative breast cancer and drug-resistant cells, which is applied in the field of biomedicine, can solve the problem of rare drug-resistant cell lines of triple-negative breast cancer, and achieve high scientific research and production application value and high drug resistance.

Inactive Publication Date: 2015-08-12
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The establishment of drug-resistant cell lines of breast cancer to doxorubicin has been commonly used at home and abroad, but drug-resistant cell lines for triple-negative breast cancer are still rare, especially the drug-resistant triple-negative breast cancer induced by DDP (cisplatin) Cell lines have not been reported

Method used

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  • Triple-negative breast cancer cisplatin drug-resistance cell line, preparation method and uses thereof
  • Triple-negative breast cancer cisplatin drug-resistance cell line, preparation method and uses thereof
  • Triple-negative breast cancer cisplatin drug-resistance cell line, preparation method and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1 Induction and Establishment of Human Triple Negative Breast Cancer Drug-resistant Cell Line MDA-MB-231 / DDP

[0033]The invention adopts the in vitro induction method of gradually increasing the concentration of DDP (cisplatin) to establish the human triple-negative breast cancer drug-resistant cell line MDA-MB-231 / DDP. Specific steps are as follows:

[0034] 1. Human triple-negative breast cancer MDA-MB-231 cell line (purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences and frozen by our laboratory), was resuscitated with L15 culture medium (containing 10% fetal bovine serum, penicillin 100U / ml, strepto Mycin 100 μg / ml) was cultivated at 37°C, 5% CO2, and 95% humidity;

[0035] 2. Take MDA-MB-231 cells in the logarithmic growth phase, change fresh culture medium, add DDP (cisplatin freeze-dried powder for injection was purchased from Qilu Pharmaceutical Co., Ltd., batch number: ZWA2A1305034A), so that the concentration is 100ng / ml, At 37°...

Embodiment 2

[0040] Embodiment 2 carries out morphological observation and biological characteristic identification to the MDA-MB-231 / DDP cell strain that application method of the present invention establishes:

[0041] 1. Inverted phase-contrast microscope to observe cell morphology

[0042] Take one bottle of MDA-MB-231 cells and MDA-MB-231 / DDP cells in the logarithmic growth phase, and observe the morphology of living cells under an inverted phase-contrast microscope after changing the medium and take pictures. It can be seen that the MDA-MB-231 cells are polygonal, the shape is relatively consistent, and the intracellular structure is uniform (such as figure 1 shown); the size and shape of MDA-MB-231 / DDP cells changed, the cells were more slender, and there were more dark substances in the cells, especially near the cell membrane (such as figure 2 shown).

[0043] 2. HPLC (High Performance Liquid Chromatography) method to detect intracellular DDP concentration

[0044] Take MDA-MB...

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Abstract

The present invention discloses a triple-negative breast cancer cisplatin drug-resistance cell line, which is named human triple-negative breast cancer drug-resistance cell line MDA-MB-231 / DDP and has the preservation number of CGMCC No.10406. The present invention further discloses a preparation method of the triple-negative breast cancer cisplatin drug-resistance cell line, and applications of the triple-negative breast cancer cisplatin drug-resistance cell line in preparation of breast cancer drug-resistance reversal drugs. According to the present invention, the triple-negative breast cancer cisplatin drug-resistance cell line can be used for analyzing the morphology and biological phenotype change of the triple-negative breast cancer cells after DDP resistance, researching the molecular mechanism of the tumor on DDP resistance, researching the tumor drug-resistance reversal method, screening other antitumor drugs, detecting tumor drug-resistance markers, screening and evaluating novel antitumor drugs and the like, and has high research values and high production application values; and experiment results verify that the drug-resistance index of the triple-negative breast cancer cisplatin drug-resistance cell line MDA-MB-231 / DDP is 11, and the triple-negative breast cancer cisplatin drug-resistance cell line MDA-MB-231 / DDP has high drug resistance and can be used for preparing breast cancer drug-resistance reversal drugs.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a drug-resistant cell line of breast cancer, in particular to a cisplatin-resistant cell line of triple-negative breast cancer and its preparation method and application. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. According to statistics, there are about 1.2 million cases of breast cancer in the world every year, and about 500,000 people die of breast cancer. With the deepening of research on breast cancer, breast cancer is currently considered to be a highly heterogeneous tumor, with different pathological and molecular characteristics leading to significantly different responses to treatment and prognosis. Estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (human epidermal growth factor receptor-2, HER-2) detected clinically by immunohistochemistry , Ki67 and other results divid...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02
Inventor 陈红风盛佳钰叶媚娜
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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