Method for quickly screening salmonella producing biological membranes

A Salmonella and biofilm technology, applied in the field of microorganisms, can solve the problems of low screening efficiency and poor specificity, and achieve the effect of high screening efficiency, low cost and ensuring accuracy

Pending Publication Date: 2018-03-20
NORTHWEST A & F UNIV
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Problems solved by technology

[0004] In order to solve the problems of poor specificity and low screening efficiency in the current screening process of producing Salmonella biofilm, the present invention discloses a method for rapidly screening and producing Salmonella biofilm

Method used

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Examples

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Embodiment Construction

[0014] The present invention is specifically implemented through the following operations:

[0015] a. Strain activation and inoculation: take out the Salmonella stored at -80°C with an inoculation loop, streak and inoculate it on the surface of sterile Luria-Bertani (LB) agar medium under sterile conditions, and incubate at 37°C for 18 hours; use inoculation under sterile conditions Take a single colony on the LB agar medium, streak it on the sterile BHI agar medium, and incubate at 37°C for 24 hours;

[0016] b. Cultivation and Congo red staining: put the BHI culture at 22°C for 24 hours under sterile conditions, absorb 5 mL of 0.08% Congo red solution, and add it to the BHI petri dish to stain the colony and bacterial lawn for 40 seconds;

[0017] c. Judgment of the results: the color of the outer circle of Salmonella biofilm colonies and bacterial lawns turns red, and the surface of the colonies and bacterial lawns appears rough; no biofilm-producing Salmonella colonies an...

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Abstract

The invention discloses a method for quickly screening salmonella producing biological membranes. The method comprises the steps: an inoculating loop is utilized to take out cryopreserved salmonella under the sterile condition, and a sterile agar culture medium is streak inoculated with the salmonella; BHI (brian heart infusion) culture is stood under the sterile condition to suck congo red dye liquor and is added into a culture dish to dye a bacterial colony and bacterial lawns; colors of outer circles of the bacterial colony and the bacterial lawns of the salmonella producing biological membranes go red, and the surfaces of the bacterial colony and the bacterial lawns are in rough shapes; the bacterial colony and the bacterial lawns of the biological membranes which cannot produce the salmonella have no obvious change; crystal violet straining comprises liquid culturing, biological membrane fixing and dying. According to the method, the congo red and a crystal violet straining methodare organically combined; thus, detection specificity is ensured, result accuracy is ensured, the purpose of specifically screening the salmonella producing biological membranes is achieved, the screening efficiency is high, cost is low, and higher scientific and practical values are achieved.

Description

technical field [0001] The invention relates to a method for screening and producing Salmonella biofilm, in particular to a method for rapidly screening and producing Salmonella biofilm, belonging to the field of microorganisms. Background technique [0002] salmonella( Salmonella ) is a zoonotic pathogen of great significance in public health. The biofilm produced by Salmonella will endow it with resistance to adverse conditions such as pH value, dryness, osmotic pressure, temperature, antibiotics and disinfectants. Salmonella can survive in the environment of acidic gastric juice, low water content, food with high sugar or salt content, which increases the probability of Salmonella reaching the human intestinal tract and causing infection, which may lead to food poisoning. In addition, once biofilm is formed, it can also weaken the killing effect of antibacterial drugs on bacteria, making antibiotic treatment ineffective. At the same time, biofilm can make Salmonella eas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12R1/42
CPCC12Q1/10G01N2333/255
Inventor 杨保伟张旭张英朔李爽曹晨阳盛敏盛焕精
Owner NORTHWEST A & F UNIV
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