Method for quickly screening salmonella producing biological membranes
A Salmonella and biofilm technology, applied in the field of microorganisms, can solve the problems of low screening efficiency and poor specificity, and achieve the effect of high screening efficiency, low cost and ensuring accuracy
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[0014] The present invention is specifically implemented through the following operations:
[0015] a. Strain activation and inoculation: take out the Salmonella stored at -80°C with an inoculation loop, streak and inoculate it on the surface of sterile Luria-Bertani (LB) agar medium under sterile conditions, and incubate at 37°C for 18 hours; use inoculation under sterile conditions Take a single colony on the LB agar medium, streak it on the sterile BHI agar medium, and incubate at 37°C for 24 hours;
[0016] b. Cultivation and Congo red staining: put the BHI culture at 22°C for 24 hours under sterile conditions, absorb 5 mL of 0.08% Congo red solution, and add it to the BHI petri dish to stain the colony and bacterial lawn for 40 seconds;
[0017] c. Judgment of the results: the color of the outer circle of Salmonella biofilm colonies and bacterial lawns turns red, and the surface of the colonies and bacterial lawns appears rough; no biofilm-producing Salmonella colonies an...
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