Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing L-tyrosine from fermentation broth of Chinese prickly ash endogenous brevibacterium frigoritolerans

A cold-resistant Brevibacterium, fermentation broth technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc.

Inactive Publication Date: 2015-07-29
SICHUAN UNIV
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the isolation and preparation of L-tyrosine from the fermentation product of endophytic Brevibacterium cold-resistant in Zanthoxylum bungeanum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing L-tyrosine from fermentation broth of Chinese prickly ash endogenous brevibacterium frigoritolerans
  • Method for preparing L-tyrosine from fermentation broth of Chinese prickly ash endogenous brevibacterium frigoritolerans
  • Method for preparing L-tyrosine from fermentation broth of Chinese prickly ash endogenous brevibacterium frigoritolerans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. A method for preparing L-tyrosine from endogenous cold-resistant Brevibacterium fermented liquid of Zanthoxylum bungeanum, characterized in that the method comprises the following steps:

[0034] (1) Microbial strains and culture medium

[0035] Strain: a strain of endophytic cold-resistant Brevibacterium GH-HJZ2 (Brevibacterium frigoritolerans GH-HJZ2, preservation number: CCTCC M2013422) strain preserved by the China Center for Type Culture Collection. Above, after culturing in a constant temperature incubator at a temperature of 30°C for 24 hours, inoculate a single colony into 150 mL of fermentation medium, and inoculate for 24 hours at a temperature of 28°C on a water bath constant temperature shaker at 120 r / min.

[0036] Brevibacterium cold-resistant slant culture medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, distilled water 1000mL, initial pH value 7.2-7.4. The fermentation medium is: 1% soluble starch, 3.5% soybean powder, 1% NaCl, and the initia...

Embodiment 2

[0046] (1) Microbial strains and culture medium

[0047] Strain: a strain of endophytic cold-resistant Brevibacterium GH-HJZ2 (Brevibacterium frigoritolerans GH-HJZ2, preservation number: CCTCC M2013422) strain preserved by the China Center for Type Culture Collection, the culture condition is streak inoculation into slant medium Above, after culturing in a constant temperature incubator at a temperature of 30°C for 24h, inoculate a single colony into 150mL fermentation medium, and inoculate for 48h at a temperature of 30°C on a water bath constant temperature shaker at 140r / min.

[0048] Brevibacterium cold-resistant slant culture medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, distilled water 1000mL, initial pH value 7.2-7.4. The fermentation medium is: 1% soluble starch, 3.5% soybean powder, 1% NaCl, and the initial pH value is 7.0.

[0049] (2) Fermentation production

[0050] Using the above-mentioned strains and cultivation methods, the endophytic Brevibacteri...

Embodiment 3

[0058] (1) Microbial strains and culture medium

[0059] Strain: a strain of endophytic cold-resistant Brevibacterium GH-HJZ2 (Brevibacterium frigoritolerans GH-HJZ2, preservation number: CCTCC M2013422) strain preserved by the China Center for Type Culture Collection, the culture condition is streak inoculation into slant medium Above, after culturing in a constant temperature incubator at a temperature of 30°C for 24h, inoculate a single colony into 150mL fermentation medium, and inoculate for 72h at a temperature of 37°C on a 180r / min water bath constant temperature shaker.

[0060] Brevibacterium cold-resistant slant culture medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, distilled water 1000mL, initial pH value 7.2-7.4. The fermentation medium is: 1% soluble starch, 3.5% soybean powder, 1% NaCl, and the initial pH value is 7.0.

[0061] (2) Fermentation production

[0062] Using the above-mentioned strains and cultivation methods, the endophytic Brevibacterium ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing L-tyrosine from a fermentation broth of Chinese prickly ash endogenous brevibacterium frigoritolerans. The method is characterized by comprising the following steps: carrying out constant temperature incubation on Chinese prickly ash endogenous brevibacterium frigoritolerans at the temperature of 30 DEG C for 24h, inoculating the strain to a fermentation medium, and carrying out constant temperature shaking fermentation culture by water bath at 28-37 DEG C and at 120-180 r / min; centrifuging the fermentation broth at normal temperature and at the speed of 4000-6000 r / min for 15 min to remove thalli so as to obtain a supernatant, and carrying out freeze-drying on the supernatant; processing the fermentation broth through a Sephadex LH-20 chromatographic column under the condition that mass ratio of the sample to Sephadex LH-20 is 1:20-50 and loading quantity of sample is 20% of the column volume, carrying out isocratic elution with 100 mL of pure water, and equally collecting eluants F1-F5 according to the collection amount of 20 mL for each eluant; and carrying out separation and purification on the component F3 by a preparative liquid chromatograph, and carrying out freeze-drying to obtain L-tyrosine with the yield being 8-15 mg / L.

Description

technical field [0001] The invention relates to a method for preparing L-tyrosine from endogenous cold-resistant Brevibacterium fermented liquid of Zanthoxylum bungeanum. [0002] The bacterial strain obtained in the present invention has been preserved in the China Center for Type Culture Collection on September 14, 2013, number: CCTCC M 2013422, and the classification name is Brevibacterium frigoritolerans GH-HJZ2. Background technique [0003] L-tyrosine is an aromatic amino acid, which is a conditionally essential amino acid. Supplementing tyrosine in an appropriate amount can stimulate brain nerves, improve memory, help improve anxiety, irritability and other emotions, and enhance body resistance. In addition, L-tyrosine is also the main raw material for the synthesis of L-dopa and melanin. L-dopa is a commonly used drug for the clinical treatment of Parkinson's disease. The traditional L-tyrosine synthesis method is to use acid, alkali or enzyme to hydrolyze protein-c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C12R1/13
Inventor 钟凯陈冲邓鹏翅李霄洁赵志峰高鸿山口五十磨
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com