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Preparation method and application of a compost low-temperature composite fermentation bacterial agent

A technology of composite fermentation bacteria and compost, applied in the field of agricultural biology, can solve the problems of ineffective fermentation of manure, large temperature difference between day and night, and long duration of low temperature

Active Publication Date: 2022-03-01
DALIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, composting is easily affected by ambient temperature, especially in Xinjiang and Northeast China. Due to the large temperature difference between day and night and the low temperature for a long time, a large amount of manure cannot be fermented effectively, which brings great challenges to the application of composting technology.
[0003] At present, in order to solve the problem of starting and stabilizing the heat preservation of compost in winter, the method of inputting hot steam and hot water, or directly laying heating devices to control and increase the temperature of compost, but these methods are expensive and consume electricity and energy, are not long-term solution

Method used

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  • Preparation method and application of a compost low-temperature composite fermentation bacterial agent
  • Preparation method and application of a compost low-temperature composite fermentation bacterial agent
  • Preparation method and application of a compost low-temperature composite fermentation bacterial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1: Activation culture of Arthrobacter tobacco, Brevibacterium cold-resistant and Helicheli spirochete

[0039] 1. The bacterial strain of the present invention is isolated from the cow dung of Dalian Xinle Dairy Cow Breeding Plant and the permafrost layer of Dalian University of Technology. Through separation and purification and biological identification, it is finally determined to be Arthrobacternicotianae, Brevibacterium cold-resistant ( Brevibacterium frigoritolerans), Herbaspirillum huttiense. Store the three strains of bacteria in a -80°C refrigerator.

[0040] 2. Preparation method of beef extract peptone solid medium:

[0041]The activation medium of Arthrobacter nicotianae, Brevibacterium cold-resistant and Helicospira spirillum includes the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride, 15g of agar, and make up water to 1000mL. Mix the above components and adjust the pH to 7.0-7.2. If the pH is high, use hydrochl...

Embodiment 2

[0043] Embodiment 2: the preparation of compost low-temperature composite fermentation bacterial agent

[0044] S1. Take the number of effective viable bacteria > 1×10 8 CFU / mL of Arthrobacter tobacco ZL-1 bacterium liquid, Brevibacterium cold-resistant ZL-2 bacterium liquid, and Helicobacter herstii ZL-3 bacterium liquid are mixed in a volume ratio of 1:1:1 to obtain a fermentation liquid;

[0045] S2. Mix the fermented liquid obtained in step S1 with the carrier in a mass ratio of 2:1, and dry it at room temperature for 48 hours to a moisture content≤25%, to obtain a compost low-temperature composite fermentation bacterial agent; Corncob powder after 20min sterilization at ℃;

[0046] Wherein, the preparation method of the Arthrobacter tobacco ZL-1 bacterium liquid described in step S1 comprises the steps of:

[0047] 1. Preparation of seed solution: inoculate the single colony of Arthrobacter nicotianae ZL-1 obtained in Example 1 into 200ml beef extract peptone liquid med...

Embodiment 3

[0055] Embodiment 3: Effect evaluation of the application of composting low-temperature compound fermentation bacterial agent

[0056] 1. Establish an evaluation system for winter cow dung composting at an ambient temperature of 5-10°C. Fully mix fresh cow dung and straw at a mass ratio of 4:1, adjust the water content to about 60%, and obtain a cow dung composting system. Inoculate the compost low-temperature composite fermentation bacterial agent prepared by the present invention at a weight ratio of 0.5% in a cow dung composting system with a length of 1 m, a width of 0.8 m, and a height of 1.2 m, and set a blank control group without adding the bacterial agent.

[0057] 2. Record the ambient temperature and the temperature of the pile every day. The temperature of the pile is measured by a piercing thermometer. The measurement position is 30cm below the height of the center of the pile. The temperature of the pile is measured once a day at 9:00 and 17:00 and the average va...

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Abstract

The invention provides a method for preparing a compost low-temperature composite fermentation bacterial agent, comprising steps: S1, taking the number of effective viable bacteria > 1 × 10 8 CFU / mL of Arthrobacter tobacco ZL‑1 bacterial liquid, Brevibacterium cold-tolerant ZL‑2 bacterial liquid, and Helispiribacter hersii ZL‑3 bacterial liquid are mixed in a volume ratio of 1:1:1 to obtain a fermented liquid; S2, step The fermented liquid obtained in S1 is mixed evenly with the corn cob powder according to the mass ratio of 2:1, and dried until the water content is ≤25%, and the compost low-temperature composite fermentation bacterial agent is obtained. The compost low-temperature composite fermentation bacterial agent prepared by the invention can be applied to the composting of fresh feces in autumn and winter, and solves the problem of environmental pollution caused by the difficulty in starting fermentation of the compost in a cold environment and the accumulation of a large amount of feces. In an environment of 5-10°C, the compost with the low-temperature compound fermentation bacteria agent added to the compost body heats up quickly and has a high temperature. It enters a high-temperature period on the 3rd to 4th day and lasts for 8-10 days. The highest temperature is 76.2°C, which has reached the level of composting in my country. harmless standard.

Description

technical field [0001] The technical field of agricultural biology of the present invention, more specifically, relates to a preparation method and application of a compost low-temperature composite fermentation bacterial agent. Background technique [0002] Composting technology is a resource utilization method of livestock and poultry manure with low cost, strong operability and low biological risk. Using this technology, organic matter can be biodegraded under the action of microorganisms, and the high temperature generated by its vigorous activities can kill pathogenic microorganisms and other harmful substances, and finally form a safe and green organic fertilizer. However, composting is easily affected by ambient temperature, especially in Xinjiang and Northeast China. Due to the large temperature difference between day and night and the low temperature for a long time, a large amount of manure cannot be fermented effectively, which brings great challenges to the appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/06
CPCC12N1/20C05F11/08C12R2001/06C12N1/205
Inventor 徐永平张喆李晓宇侯扶琴陈红莉吴妍妍陈岩王丽丽王思佳
Owner DALIAN UNIV OF TECH
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