Preparation method and application of a compost low-temperature composite fermentation bacterial agent
A technology of composite fermentation bacteria and compost, applied in the field of agricultural biology, can solve the problems of ineffective fermentation of manure, large temperature difference between day and night, and long duration of low temperature
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Embodiment 1
[0038] Embodiment 1: Activation culture of Arthrobacter tobacco, Brevibacterium cold-resistant and Helicheli spirochete
[0039] 1. The bacterial strain of the present invention is isolated from the cow dung of Dalian Xinle Dairy Cow Breeding Plant and the permafrost layer of Dalian University of Technology. Through separation and purification and biological identification, it is finally determined to be Arthrobacternicotianae, Brevibacterium cold-resistant ( Brevibacterium frigoritolerans), Herbaspirillum huttiense. Store the three strains of bacteria in a -80°C refrigerator.
[0040] 2. Preparation method of beef extract peptone solid medium:
[0041]The activation medium of Arthrobacter nicotianae, Brevibacterium cold-resistant and Helicospira spirillum includes the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride, 15g of agar, and make up water to 1000mL. Mix the above components and adjust the pH to 7.0-7.2. If the pH is high, use hydrochl...
Embodiment 2
[0043] Embodiment 2: the preparation of compost low-temperature composite fermentation bacterial agent
[0044] S1. Take the number of effective viable bacteria > 1×10 8 CFU / mL of Arthrobacter tobacco ZL-1 bacterium liquid, Brevibacterium cold-resistant ZL-2 bacterium liquid, and Helicobacter herstii ZL-3 bacterium liquid are mixed in a volume ratio of 1:1:1 to obtain a fermentation liquid;
[0045] S2. Mix the fermented liquid obtained in step S1 with the carrier in a mass ratio of 2:1, and dry it at room temperature for 48 hours to a moisture content≤25%, to obtain a compost low-temperature composite fermentation bacterial agent; Corncob powder after 20min sterilization at ℃;
[0046] Wherein, the preparation method of the Arthrobacter tobacco ZL-1 bacterium liquid described in step S1 comprises the steps of:
[0047] 1. Preparation of seed solution: inoculate the single colony of Arthrobacter nicotianae ZL-1 obtained in Example 1 into 200ml beef extract peptone liquid med...
Embodiment 3
[0055] Embodiment 3: Effect evaluation of the application of composting low-temperature compound fermentation bacterial agent
[0056] 1. Establish an evaluation system for winter cow dung composting at an ambient temperature of 5-10°C. Fully mix fresh cow dung and straw at a mass ratio of 4:1, adjust the water content to about 60%, and obtain a cow dung composting system. Inoculate the compost low-temperature composite fermentation bacterial agent prepared by the present invention at a weight ratio of 0.5% in a cow dung composting system with a length of 1 m, a width of 0.8 m, and a height of 1.2 m, and set a blank control group without adding the bacterial agent.
[0057] 2. Record the ambient temperature and the temperature of the pile every day. The temperature of the pile is measured by a piercing thermometer. The measurement position is 30cm below the height of the center of the pile. The temperature of the pile is measured once a day at 9:00 and 17:00 and the average va...
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