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A kind of Brevibacterium prickly ash and its screening and purification method and application

A technology of endophyte and Brevibacterium cold-resistant, applied in the directions of biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve the problem of no oxidation resistance of the metabolites of Zanthoxylum bungeanum endophytes, and no patent documents of Zanthoxylum bungeanum endophytes. and non-patent literature reports, etc., to achieve the effects of convenient operation, good antioxidant activity, and simple screening method

Inactive Publication Date: 2016-07-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no patent literature and non-patent literature reports on the endophytic bacteria of Zanthoxylum bungeanum, and there is no research report on the antioxidant activity of the metabolites of the endophytic bacteria of Zanthoxylum bungeanum

Method used

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  • A kind of Brevibacterium prickly ash and its screening and purification method and application
  • A kind of Brevibacterium prickly ash and its screening and purification method and application
  • A kind of Brevibacterium prickly ash and its screening and purification method and application

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Effect test

Embodiment 1

[0038] (1) cleaning and disinfection

[0039] In the aseptic operating table, soak the fresh red pepper fruit from Hanyuan with 1% sodium hypochlorite for 10 minutes, disinfect with 75% ethanol for 10 minutes, rinse with sterile water three times, and keep the water for the last washing as a control.

[0040] (2) Enrichment culture

[0041] The Zanthoxylum bungeanum fruit after surface disinfection was ground and inoculated on a nutrient agar medium plate, and cultured in a constant temperature incubator at a temperature of 28° C. for 7 days.

[0042] (3) flat plate coating

[0043] Pick bacterial colonies from the colonies cultured on nutrient agar solid medium, dilute them in sterile saline, spread them on a plate, place them in a constant temperature incubator at 28°C for 7 days, and then pick bacterial colonies on nutrient agar solids Streak the plate on the culture medium and place it in a constant temperature incubator at a temperature of 28°C for 7 days.

[0044] (4)...

Embodiment 2

[0051] (1) Cleaning and disinfection:

[0052]In the aseptic operation bench, soak the fresh red pepper fruit from Hanyuan with 2% sodium hypochlorite for 7 minutes, disinfect with 75% ethanol for 13 minutes, rinse with sterile water three times, and keep the water for the last washing as a control.

[0053] (2) Enrichment culture: Grind the Zanthoxylum bungeanum fruit after surface disinfection, inoculate it on a nutrient agar medium plate, and culture it in a constant temperature incubator at a temperature of 29° C. for 6 days.

[0054] (3) flat plate coating:

[0055] Pick bacterial colonies from the colonies cultured on nutrient agar solid medium, dilute them in sterile saline, spread them on a plate, place them in a constant temperature incubator at 29°C for 6 days, and then pick bacterial colonies on nutrient agar solids Streak the plate on the culture medium and place it in a constant temperature incubator at 29°C for 6 days.

[0056] (4) Primary screening:

[0057] ...

Embodiment 3

[0062] (1) Cleaning and disinfection:

[0063] In the aseptic operation bench, soak the fresh red Zanthoxylum bungeanum fruit from Hanyuan with 3% sodium hypochlorite for 3 minutes, disinfect with 75% ethanol for 15 minutes, rinse with sterile water three times, and keep the water for the last washing as a control.

[0064] (2) Enrichment culture:

[0065] The Zanthoxylum bungeanum fruit after surface disinfection was ground and inoculated on a nutrient agar medium plate, and cultured in a constant temperature incubator at a temperature of 30° C. for 5 days.

[0066] (3) flat plate coating:

[0067] Pick bacterial colonies from colonies cultured on nutrient agar solid medium, serially dilute them in sterile saline, spread them on plates, place them in a constant temperature incubator at 30°C for 5 days, and then pick bacterial colonies on nutrient agar solids After streaking the plate on the culture medium, place it in a constant temperature incubator at a temperature of 30°...

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Abstract

The invention discloses an endophytic curtobacterium of zanthoxylum as well as a screening and purifying method and application of the endophytic curtobacterium of the zanthoxylum. The screening and purifying method is characterized by comprising the steps of with a fresh zanthoxylum fruit as the raw material, inhibiting the growth of fungi by using potassium dichromate, and then, screening and purifying by using methods such as sterilizing and cleaning, plate enrichment culture, gradient dilution plate coating, primary screening, secondary screening and the like. The endophytic curtobacterium of the zanthoxylum is determined as a novel subspecies of Brevibacterium frigoritolerans. The endophytic curtobacterium obtained by the invention is wide in carbon source selection range, and a fermentation metabolic product of the endophytic curtobacterium has oxidation resistance, so that the endophytic curtobacterium of the zanthoxylum has favorable application prospect in the aspect of production of a natural antioxidant.

Description

technical field [0001] The invention relates to a Brevibacterium prickly ash and its screening and purification method and application. [0002] The bacterial strain obtained in the present invention has been preserved in the China Center for Type Culture Collection on September 14, 2013, number: CCTCCM2013422, classification name: Brevibacterium frigoritolerans GH-HJZ2 (Brevibacterium frigoritolerans GH-HJZ2). Background technique [0003] Natural antioxidants have a wide range of applications and few side effects, so they have always attracted people's attention. Extracting natural antioxidant active substances from plants has been a research hotspot for many scholars. With the enhancement of people's awareness of environmental protection, it is the general trend to extract natural antioxidant substances in a way that does not damage plants. The growth environment of plants is difficult to simulate, the growth cycle is long, and many plants are on the verge of extinction. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/13
Inventor 钟凯李霄洁田苗高鸿山口五十磨
Owner SICHUAN UNIV
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