Analysis method for simultaneously determining six polyphenol contents in flue-cured tobacco

Abstract

The invention discloses an analysis method for simultaneously determining six polyphenol content in flue-cured tobacco. The method comprises the following steps: grinding leaves of flue-cured tobacco, extracting through a methanol solution, analyzing filtrate through a high performance liquid chromatography after filtering, adopting a HSST3 chromatographic column and a diode array detector, wherein the detection wavelength of the detector is 342mm, the reference wavelength is 480nm, the bandwidth of the detection wavelength is 4nm, the bandwidth of the reference wavelength is 100nm, and the flow velocity of the flowing phase is 0.4mL / min; the flowing phase A is aqueous solution of glacial acetic acid, and the flowing phase B is methanol solution of glacial acetic acid, simultaneously determining contents of six polyphenols in the flue-cured tobacco: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, scopoletin, rutin and kaempferol alcohol-3-rutinoside in a gradient elution manner. The adding standard recovery of the method is between 95.6-109.3%, the detection limit is 3.7-9.9 migrogram / g, the relative standard deviation is 0.5-3.9% (n equals to 4), the relative coefficients of the standard solution are more than 0.9995, and the pre-treatment of the method is simple and the analysis time is short.

Description

technical field [0001] Embodiments of the present invention relate to the technical field of chemical component analysis of tobacco leaves, and more specifically, embodiments of the present invention relate to an analysis method for simultaneously determining the contents of six polyphenols in flue-cured tobacco. Background technique [0002] Tobacco contains a large number of complex phenolic compounds, more than 280 of which have been identified in tobacco leaves and smoke. According to the number of hydroxyl groups in phenolic compounds, they can be divided into two types: simple phenols and polyphenols. There are many types of simple phenols in tobacco, but the content is very small. There are relatively few types of polyphenols, but their high content is also important. This is because the polyphenols in tobacco generally have a pleasant aroma, and can produce a variety of aromatic components due to dry distillation, oxidation, and cracking during burning and smoking....

AI Technical Summary

Problems solved by technology

[0004] The present invention overcomes the deficiencies of the prior art, and provides an analysis method for simultaneously determining the contents of six polyphenols in flue-cured tobacco by using ultra-high performance liquid chromatography, and solves the problems of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, scopoletin, Problems of Complicated Analytical Methods and Inaccurate Results of Six Kinds of Polyphenols Content of Rutin and Camphenyl-3-rutinoside

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