Multi-PCR detection kit and method for identifying poultry salmonella

Abstract

The invention discloses a multi-PCR detection kit for identifying poultry salmonella. The multi-PCR detection kit comprises PCR buffer fluid, a deoxyribonucleoside triphosphate mixture, Taq DNA polymerase and primer pairs of a bcf gene, a heli gene, an rhs gene, an sdf gene, a fla gene. The invention further discloses a multi-PCR detection method for identifying the poultry salmonella through the kit. The multi-PCR detection kit can effectively determine whether the salmonella is included in a sample to be detected or not, and can further identify different epidemic salmonella serotypes in poultry production or poultry products. Through a PCR, the salmonella can be detected from the sample at the same time, the different salmonella serotypes are further identified, the detection reaction sensitivity is high, high specificity is achieved, the detection process is fast and efficient, and the kit and method are suitable for batch detection.

Description

technical field [0001] The invention belongs to the technical field of PCR detection, and in particular relates to a multiplex PCR detection kit and a method for identifying avian-derived Salmonella. Background technique [0002] Salmonella is a common zoonotic pathogen. The infection and contamination of some serotypes of Salmonella during poultry production seriously affects poultry production efficiency and endangers the poultry industry. In food poisoning incidents around the world, the poisoning cases caused by Salmonella It has risen to the first or second place, among which Salmonella infection of poultry is one of the important media transmitted to humans through the food chain. According to the US CDC and FDA yearbook reports, epidemiological investigations in poultry production in the past five years have shown that in clinical samples of poultry production, Salmonella Enteritidis is the most prevalent serotype, while Salmonella Heidelberg has the highest detection...

AI Technical Summary

Problems solved by technology

Bacteriological detection is usually based on the growth characteristics of Salmonella. Selective medium is used to make Salmonella the dominant bacterial group. A series of biochemical and serological tests are carried out on the colony isolates to make identification. The whole process of traditional Salmonella detection takes at least 4-7 days, time-consuming and labor-intensive; the immunological detection method detects Salmonella according to the surface antigen characteristics of the bacteria and the specificity of the antigen-antibody reaction, and the level of individual antibodies greatly affects the accuracy of the detection

At present, there are also PCR and other detection methods established for some serotypes of Salmonella. Products on the market (Salmonella PCR Rapid Detection Kit-Beijing Fudean Technology Co., Ltd.; Salmonella (salmonella.spp) Fluorescence Quantitative PCR Detection Kit-DuPont China -mainly used for the detection of Salmonella in food), based on the species-specific gene fragments of Salmonella, use PCR reaction to determine whether the target band is contained by nucleic acid electrophoresis, and then achieve the purpose of detecting whether the sample contains Salmonella; some technologies are Identification of a single Salmonella serotype technique (such as a detection method for Salmonella Enteritidis (S.Enteritidis)), but the above are all single PCR; while other multiplex PCR techniques are to identify different species of pathogenic bacteria at the same time, such as: Salmonella, production single Listeria nucleocytogenes, Escherichia coli multiplex PCR rapid detection kit

Images