A kind of rapid propagation method of hemp vine
A fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of natural reproduction ability, weak spreading and spreading ability, not easy to absorb water, scarcity of excellent seedlings, etc. To achieve the effect of shortening the process of seedling cultivation, short time for plant growth, and broad prospects for industrialization development
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[0028] A kind of rapid propagation method of oleifera vine: concrete steps are as follows
[0029] 1) Material selection and disinfection: pick the mature fruits of the year, remove the peel, take out the seeds and rinse them with running water, soak them in saturated bleach supernatant for 15 minutes, and scrub them with a soft brush. After rinsing, rinse them under tap water for 1 hour, and double steam Rinse with water twice, place in an ultra-clean workbench and sterilize with 75% alcohol for 30 seconds, add 0.1% mercuric chloride for 12 minutes, rinse with sterile water three times, dry the surface with sterile filter paper and inoculate;
[0030] 2) Induction culture: the sterilized seeds are inoculated in the bud induction medium sterilized by high temperature and high pressure; culture conditions: the temperature of the culture room is 23°C, the light time is 10h / d, the light intensity is 1200lx, and the culture time is 18d ;
[0031] 3) Proliferation culture: cut the...
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