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AFLP finger-print chromatogram construction of metalaxyl resistance by phytophthora sojae

A technology of Phytophthora soybean, fingerprinting, applied in the direction of microbe-based methods, microbiological measurement/testing, microbiology, etc., can solve problems such as low specificity, difficult application, and difficult to reach the practical application level

Inactive Publication Date: 2015-07-01
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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Problems solved by technology

In recent years, there have been many reports on the detection of Phytophthora (including Phytophthora soybean) using serum techniques, but there are many difficulties in the practical application of serological methods, mainly because the antiserum titer of polyclonal antibodies is not high and the specificity is not strong , it is difficult to reach the practical application level; the monoclonal antibody is too single (maybe at the genus level, or at the species level or even at the physiological small species level), and it may be missed in actual application, making it difficult to apply

Method used

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  • AFLP finger-print chromatogram construction of metalaxyl resistance by phytophthora sojae
  • AFLP finger-print chromatogram construction of metalaxyl resistance by phytophthora sojae
  • AFLP finger-print chromatogram construction of metalaxyl resistance by phytophthora sojae

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Experimental program
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Embodiment Construction

[0043] Experimental material preparation process

[0044] 1 experimental strain

[0045] 1.1 Isolation of soil strains

[0046] 1.1.1 Collection location: Phytophthora soybean outbreak site in Lindian area, Daqing City, Heilongjiang Province.

[0047] 1.1.2 Collection method:

[0048] 1.1.3 Examination of diseased tissue: Cut the diseased tissue into small pieces of 1 cm, place it in 10% KOH aqueous solution, and place it at room temperature for 4 hours. After the diseased tissue is transparent, peel off the epidermis, and observe the pathogens in the tissue under a microscope.

[0049] 1.1.4 Soil preparation: Air-dry the collected diseased soil at room temperature, crush it, sieve it (aperture 2mm), and store it in a refrigerator at 4°C for 30-60 days, so that P.s in the diseased soil exists in the form of oospores .

[0050]1.1.5 Leaf disc trapping method: the test leaf disc is an American variety that does not give up the Rps gene. Weigh 5g of diseased soil, 5g of ster...

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Abstract

The invention provides an AFLP finger-print chromatogram construction of metalaxyl resistance by phytophthora sojae, which belongs to the phythophthora sojae variation detection field. The invention is characterized in that a stable mutant for resistance of metalaxyl by phytophthora sojae is employed, AFLP finger-print chromatogram construction is carried out to obtain specific bands, by constructing the AFLP finger-print chromatogram of metalaxyl resistance by phytophthora sojae, and the invention provides an effective durable resistance species.

Description

【Technical field】 [0001] The invention relates to the construction of the AFLP fingerprint of the resistance of Phytophthora soybean to metalaxyl. 【Background technique】 [0002] Phytophthorasojae (Phytophthorasojae) can cause Phytophthora sojae root rot, which is extremely harmful in soybean production. Soybean blight has become a prominent problem in China's soybean production and has brought serious economic losses to my country's soybean production. [0003] Phytophthora soybean is a soil-borne fungus, but the pathogen is highly parasitic and cannot compete for growth and colonization on soil particles, and oospores can survive for many years in plant residues and soil. Phytophthora sojae on lima beans, corn flour, V 8 It grows faster on medium such as juice, and the colonies are uniform and the edges are neat. Mycelium is 3-9 μm wide, easy to curl; mycelium is coral-like, with nearly right-angled branches, slightly constricted at the base of branches, and septa are f...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/645
Inventor 刘淑霞潘冬梅杜春玉魏国江沈玉江国莉玲高宇韩喜财宋鑫玲
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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