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Bacillus subtilis YBM-4 and application thereof in preventing and controlling tobacco black shank and promoting growth

A Bacillus subtilis, YBM-4 technology, applied in the application, pest control, plant growth regulator and other directions, can solve problems such as insufficient control of tobacco black shank, and achieve difficult drug resistance, good biocontrol effect, and good antagonism active effect

Inactive Publication Date: 2017-05-31
中国烟草总公司海南省公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is also commercialized Bacillus subtilis at present, but there are still deficiencies in the prevention and treatment of tobacco black shank and growth promotion

Method used

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  • Bacillus subtilis YBM-4 and application thereof in preventing and controlling tobacco black shank and promoting growth
  • Bacillus subtilis YBM-4 and application thereof in preventing and controlling tobacco black shank and promoting growth
  • Bacillus subtilis YBM-4 and application thereof in preventing and controlling tobacco black shank and promoting growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Screening of Bacillus subtilis YBM-4 and its antagonism to black shank

[0044] 1.1 Isolation and screening of antagonistic bacteria:

[0045] A total of 87 soils were collected from Kaiyang, Qingzhen, Guizhou, Panzhihua, Xichang, Sichuan, Zhucheng, Yishui, Jimo, Shandong, and other places. In order to obtain highly effective antagonistic bacteria, the root soil of black shank diseased plants at a depth of 5-10cm and the adjacent soil were selected. The root soil of healthy plants was marked and stored in a -20°C refrigerator until use.

[0046]The 87 parts of soil collected were treated as follows: firstly, the roots, stems, leaves and other sundries in the soil were removed, crushed and passed through a 20-mesh sieve. Weigh 5g of the treated soil with an electronic balance, put it into a 100mL Erlenmeyer flask, add water to make up to 50mL, shake evenly, take 1mL of the supernatant and dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 times. Use a p...

Embodiment 2

[0067] Example 2 Pot experiment on control effect of Bacillus subtilis YBM-4 on controlling tobacco black shank

[0068] Shake cultured YBM-4 bacteria solution, the concentration of the bacteria solution is about (2±0.5)×10 8 cfu / mL, after diluting 100 times, root-irrigate the potted tobacco plants that have grown to 5-6 true leaves, and at the same time inoculate the tobacco plants with blackleg bacteria under the same conditions: scratch lightly with a knife Injure the roots of tobacco plants, and inoculate the cultured blackleg bacteria into the wound. The medicine was applied every 7 days for a total of 3 times. The control agent is sprayed with 58% nail cream · manganese zinc wettable powder and 100 billion / g Bacillus subtilis powder respectively 500 times liquid to inoculate the tobacco strain of black shank bacteria, and the blank control is treated with an equal amount of sterile water, and the others are the same. See Table 1 for specific operations. 20 plants per ...

Embodiment 3

[0086] Example 3 Growth-promoting test of Bacillus subtilis YBM-4

[0087] In order to verify the growth-promoting effect of YBM-4 antagonistic bacteria on tobacco plants, first, the tobacco seedlings that had grown to 5-6 true leaves were removed from the false planting trays, and the test antagonistic bacteria liquid prepared in advance (1 × 10 6 cfu / mL), take it out after 30 min and transplant it into a flowerpot filled with sterilized soil. After 3 days of transplanting the slow seedlings, the roots of the tobacco plants were respectively treated with antagonistic antibacterial liquid, 10 mL / plant, 10 plants each time, repeated 3 times, and the blank control was treated with the same amount of water. Cultivate and observe in a greenhouse with a temperature of 28°C and a humidity of about 80%. At 15d, 30d, and 45d after transplanting, 10 representative tobacco plants were randomly selected from 30 plants, and the growth-promoting indicators were recorded, and the fresh wei...

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Abstract

The invention relates to a bacillus subtilis YBM-4, of which the classification name is Bacillus subtilis. The bacillus subtilis YBM-4 is collected at China General Microbiological Culture Collection Center (CGMCC) on April 15th, 2016 at No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the collection number of CGMCC No.12355. The bacterial strain has the following advantages: (1) excellent biocontrol effect: in a pot experiment, a YBM-4 bacterial solution is used for treating and inoculating a tobacco plant suffering from tobacco black shank, the preventing and controlling effect can reach up to 84.33% and is obviously better than the preventing and controlling effect of 58% metalaxyl manganese zinc and biological agent bacillus subtilis, the bacillus subtilis has a potential application value at the aspect of biological prevention and control of the tobacco black shank, the development and application prospects are excellent, the biological prevention and control are environment-friendly, difficult to generate drug resistance and safe to human and livestock, and conditions are created for the development of green prevention and control of the tobacco; and (2) excellent growth promoting effect: the biomass and agronomic trait of the YBM-4 bacteria tobacco plant have an obvious growth promoting effect and are obviously different from a control group.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a bacillus subtilis YBM-4 and its application in preventing and controlling tobacco black shank and promoting growth. Background technique [0002] Tobacco black shank fungus (Phytophthora parasitica var. nicotianae) is a facultative parasite that is devastating to tobacco. Black shank bacteria like high temperature and high humidity. When the temperature is 28-30 ℃ and the humidity reaches above 80%, it will create favorable conditions for the release of sporangia and the spread of zoospores. At this time, it is most likely to cause the epidemic of tobacco black shank And occur, causing a large area of ​​tobacco plant death. Tobacco, as its host plant, reaches its peak during the period from the cluster stage to the vigorous growth period, and mainly damages the roots and stem bases of tobacco plants. In addition, the continuous cropping of the land for ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/04A01P3/00A01P21/00C12R1/125
CPCA01N63/30C12N1/205C12R2001/125
Inventor 肖丽娜陈德鑫李锡宏黎妍妍徐同伟廖勇邓兆权任春燕胡兴李余江
Owner 中国烟草总公司海南省公司
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