Troponin I detection kit and detection method

Abstract

The invention relates to the technical field of fluorescence immune chromatography in medical immunology, and particularly relates to a troponin I detection kit and a troponin I detection method. The troponin I detection kit is provided with a test paper card, and is characterized in that the test paper card is provided with a PVC plate, a sample mat, a combination mat, a nitrocellulose membrane and a water absorption mat from bottom to top, wherein the combination mat adsorbs a troponin I monoclonal antibody marked with rare-earth fluorescent micro-spheres, the diameter of each rare-earth fluorescent micro-sphere is 60nm to 120nm, the rare-earth fluorescent micro-spheres are doped with a rare-earth lanthanide element and are stable at a ground state, and the fluorescence with the wavelength range of 540nm to 600nm can be transmitted under the effect of a 340-380nm excitation light source; the monoclonal antibody is in a (2+2) mode, namely, each of a capturing antibody and a detection antibody is a mixture of two high-quality monoclonal antibodies so as to simultaneously guarantee the specificity and affinity. The troponin I detection kit has advantages of simple operation, rapid reaction, high sensitivity, high specificity and the like.

Description

technical field [0001] The invention relates to the technical field of fluorescent immunochromatography in medical immunology, in particular to a troponin I detection kit and a detection method capable of fast and accurate quantitative analysis of troponin I. Background technique [0002] About 17 million people die from cardiovascular diseases every year in the world, and more than half of them die from acute myocardial infarction (AMI). There are about 10 million myocardial infarction patients each year, of which about 3 million are ST-segment elevation acute myocardial infarctions. After myocardial cell injury, the integrity and permeability of the membrane change, and macromolecular substances in the cells escape and can be detected in the blood circulation. These macromolecular substances are called myocardial injury markers, referred to as myocardial markers. The detection of myocardial markers can directly affect the clinical diagnosis, risk stratification, treatment...

AI Technical Summary

Problems solved by technology

The ELISA method has poor quantitative accuracy, long operation time and low degree of automation, and is mostly used for qualitative detection; the sensitivity of radioimmunoassay method can reach 4pg / ml, and can detect normal human serum cTnI, which is more sensitive than double-antibody sandwich method. The disadvantage is that it takes longer time. Long, the test results are unstable, the repeatability is worse than ELISA, and there is a risk of radioactive contamination; latex particle enhanced immunoturbidimetry (PETIA) can be easily applied to automatic biochemical analyzers, but the sensitivity is low and cardiomyocytes cannot be detected The gold standard method has low sensitivity, generally only qualitative, not quantitative, especially the shortcoming of poor repeatability limits its clinical application, especially not suitable for the diagnosis of diseases that need accurate quantification Quantitative detection of body fluid marker proteins; immunoluminescence method has strong specificity and high sensitivity, but requires expensive equipment and experienced operators, and is generally used in specific medical institutions

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