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Oxytetracycline sensing detection method based on graphene aptamer hydrogel

A detection method, graphene technology, applied in the field of environmental analysis, can solve the problems of expensive instruments and cumbersome steps, and achieve the effects of high selectivity, strong anti-interference, and simple operation

Inactive Publication Date: 2015-06-24
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods mainly have the advantages of high sensitivity and strong specificity, but generally require expensive instruments, cumbersome steps and sample pretreatment

Method used

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  • Oxytetracycline sensing detection method based on graphene aptamer hydrogel
  • Oxytetracycline sensing detection method based on graphene aptamer hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A method for sensing and detecting oxytetracycline based on graphene aptamer hydrogel.

[0026] Accurately weigh 2g of graphite powder, add to 20ml of concentrated sulfuric acid, stir, and let stand for 12h. Slowly add 5g KMnO 4 , and ultrasonic 8h. Then slowly add 30ml of water, stir, add 150ml of water after cooling down, and add 20ml of 30% hydrogen peroxide solution. At this time, the system turns from brown to golden yellow immediately, which means the synthesis is complete. To purify the synthesized GO, first add 2mol / L dilute hydrochloric acid, centrifuge at 7000r / min and discard the upper solution, repeat twice, and then repeat the above steps 5 times with high-purity water. Finally, put it into a 10,000 Dalton dialysis bag and dialyze for 1-2 weeks.

[0027] The graphene solution concentration was 1 mg / ml, and the aptamer concentration was 0.5 mg / ml. Since the hydrothermal synthesis method of this method takes a long time, the graphene oxide solu...

Embodiment 2

[0029] Example 2: A method for sensing and detecting oxytetracycline based on graphene aptamer hydrogel

[0030] Accurately weigh 1.5g of graphite powder, add to 28ml of concentrated sulfuric acid, stir, and let stand for 12h. Slowly add 4.5g KMnO 4 , and ultrasonic 8h. Then slowly add 40ml of water and stir. After cooling down, add 130ml of water and 18ml of 30% hydrogen peroxide solution. At this time, the system changes from brown to golden yellow immediately, which means the synthesis is complete. To purify the synthesized GO, first add 1.5mol / L dilute hydrochloric acid, centrifuge at 7500r / min and discard the upper solution, repeat twice, and then repeat the above steps 5 times with high-purity water. Finally, it was put into an 8000 Dalton dialysis bag and dialyzed for 2 weeks.

[0031] The graphene solution concentration is 1.5 mg / ml, and the aptamer concentration is 0.8 mg / ml. Since the hydrothermal synthesis method of this method takes a long time, the graphene ox...

Embodiment 3

[0033] Example 3: A method for sensing and detecting oxytetracycline based on graphene aptamer hydrogel.

[0034] Accurately weigh 1.25g of graphite powder, add to 23ml of concentrated sulfuric acid, stir, and let stand for 10h. Slowly add 4.5g KMnO 4 , and ultrasonic 8h. Then slowly add 35ml of water, stir, add 145ml of water after cooling down, and add 18ml of 30% hydrogen peroxide solution. At this time, the system turns from brown to golden yellow immediately, which means the synthesis is complete. To purify the synthesized GO, first add 1.5mol / L dilute hydrochloric acid, centrifuge at 7500r / min and discard the upper solution, repeat twice, and then repeat the above steps 5 times with high-purity water. Finally, it was put into a 10,000 Dalton dialysis bag and dialyzed for 2 weeks.

[0035] The graphene solution concentration was 0.9mg / ml, and the aptamer concentration was 0.4mg / ml. Since the hydrothermal synthesis method of this method takes a long time, the graphene ox...

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Abstract

The invention provides a colorimetric sensing method for detecting low-concentration oxytetracycline in water, belonging to the field of environment analysis. Graphene aptamer hydrogel meeting the detection requirement is synthesized, and a three-dimensional structure is represented by figures in the abstract. A scanning electronic microscope is used for observing the morphology, and the performance and the specificity of oxytetracycline in the detection solution are tested. A result shows that the method has the performance of qualitatively and quantitatively detecting an oxytetracycline sample solution within the concentration range of 50ppb and 1ppm; the qualitative detection is sensitive to reaction and convenient to observe; the quantitative detection is high in relevance; antibiotics such as carubicin, isochlortetracycline hydrochloride, sulfamethoxazole and erythrocin and oxytetracycline can be specifically distinguished.

Description

technical field [0001] The invention belongs to the field of environmental analysis and relates to a colorimetric sensing detection method for oxytetracycline in aqueous solution. Background technique [0002] In recent years, oxytetracycline, as a common antibiotic drug, has been widely used in the treatment of human and various animal diseases, as well as additives in animal husbandry and fish farming. Relevant medical research shows that the metabolism of the human body and other animals cannot completely decompose oxytetracycline, and the remaining oxytetracycline enters the environment through the metabolism of animals. What's more serious is that currently widely used water pollution control technologies seldom carry out targeted regulation on antibiotic pollution, so the content of various antibiotics in treated sewage is still at a relatively high level. [0003] Aiming at the increasingly serious contamination of antibiotics, various analytical and detection method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 赵慧敏杜磊全燮陈硕
Owner DALIAN UNIV OF TECH
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