Preparation method of anticoagulant, anticoagulant and method for preparing blood smear using same

Abstract

The invention provides a preparation method of an anticoagulant, an anticoagulant and a method for preparing a blood smear using the anticoagulant. The preparation method comprises: taking heparin sodium and distilled water respectively, and then fully dissolving the heparin sodium in the distilled water to obtain an anticoagulant mother liquor; The low-volume syringe is quantitatively injected into the high-capacity syringe with the pre-baked anticoagulant diluent; the high-capacity syringe containing the pre-baked anticoagulant diluent is dried at 40-60°C for use. The method for using the anticoagulant to prepare a blood smear includes: collecting blood with a high-capacity syringe equipped with an anticoagulant; mixing the high-capacity syringe after blood collection; taking the blood obtained in step f, and preparing a blood smear with a glass slide . Using the anticoagulant preparation method provided in this application, the anticoagulant and the method for preparing a blood smear using the same, inexperienced students can also obtain a clear blood smear, which truly reproduces the morphological structure and characteristics of blood cells.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method of an anticoagulant, an anticoagulant and a method for preparing a blood smear using the anticoagulant. Background technique [0002] The production of blood smears is widely used clinically. The preparation of blood smears with suitable thickness, uniform distribution and good staining is one of the important basic techniques of hematological examination. [0003] In the production of blood smears, pushing the smear is the difficulty and key in the whole production technology. In the current undergraduate teaching experiment, there is an experiment of making and observing blood smears. At present, the main method used in the laboratory is to directly collect blood and directly make blood smears, but for the vast majority of students, it is the first time for them to push the slides, without the slightest experience, they often drop blood on the slides , before p...

AI Technical Summary

Problems solved by technology

At present, the main method used in the laboratory is to directly collect blood and directly make blood smears, but for the vast majority of students, it is the first time for them to push the slides, without the slightest experience, they often drop blood on the slides , it is too late to push the slide, the blood has coagulated on the slide, so it is necessary to use the blood added with a small amount of anticoagulant to make the blood smear

[0004] In actual operation, the exact dose of anticoagulant is often difficult to grasp with less blood volume. In actual operation, due to the accuracy of anticoagulant dose many times, red blood cells in the blood smears made Severely deformed, which led to the complete failure of the entire experiment

This is because the amount of blood used to make a blood smear is very small, and the amount of anticoagulant added is also required to be very small. In this case, the amount added is required to be very precise. Excessive amount will damage the cells. The shape of red blood cells, especially red blood cells, will be severely deformed and jagged; if the dosage is too small, the anticoagulant effect will not be achieved

However, if such a small precise dosage is to be directly used and directly added, it needs to be measured by a very precise instrument, which is expensive; if the conventional method is used for dissolution and constant volume, it is not only time-consuming, but also requires abundant operating experience

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