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Lactobacillus plantarum and uses thereof

A technology of Lactobacillus plantarum and medicine, applied in the field of microorganisms, can solve the problems of lactic acid bacteria and related products that need to be deepened

Active Publication Date: 2015-06-10
深圳华大基因农业控股有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the research on lactic acid bacteria and related products with probiotic function still needs to be in-depth

Method used

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  • Lactobacillus plantarum and uses thereof
  • Lactobacillus plantarum and uses thereof
  • Lactobacillus plantarum and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Separation and screening

[0023] (1) Separation and purification: Taking various traditional fermented foods as samples, the specific operation is as follows: After gradiently diluting the sample with sterile physiological saline, spread it on MRS solid medium (recipe: peptone 10.0g, beef extract 10.0g , yeast extract 5.0g, diammonium hydrogen citrate ((NH 4 ) 2 HC 6 h 5 o 7 ) 2.0g, glucose (C 6 h 12 o 6 ·H 2 (O) 20.0g, Tween 801.0ml, sodium acetate (CH 3 COONa·3H 2 O) 5.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0 g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g, manganese sulfate (MnSO 4 ·H 2 O) 0.25g, distilled water 1000ml, agar 18.0g), culture in a 37°C incubator for 24-48 hours, observe the morphological characteristics of the colonies, and pick the suspected colonies of lactic acid bacteria on the MRS solid medium, and transfer them to the MRS liquid culture Pure culture medium (recipe: omit agar from MRS solid medium form...

Embodiment 2

[0025] Example 2: Determination of Physiological and Biochemical Indexes to Identify Bacteria

[0026] Carbohydrate determination was performed on the Y-9 isolated in Example 1 using carbohydrate identification reagent strips (manufacturer name: bioMerieux, sa, version number: 07945F-CN-2002 / 11).

[0027]Purpose and principle: API50CH adopts 50 biochemical tests to study the carbohydrate metabolism of microorganisms. The API50CH test strip consists of 50 microwells and is used to study the fermentation of carbohydrate and its derivative substrates. Inoculate the test strip with API50CHL medium, hydrolyze the substrate, and then detect the fermentation test. During the incubation process of the test strip, acid is produced in the small tube on the test strip under anaerobic environment, and the pH changes to change the color of the indicator in the medium, so that the fermentation can be observed. The first well of the test strip does not contain any active ingredient and ser...

Embodiment 3

[0045] Embodiment 3: 16S rDNA strain identification

[0046] The bacterial strain obtained in Example 1 was isolated, and the 16S rDNA strain identification was carried out according to the following steps:

[0047] (1) Use a sterilized inoculation loop to dip in the bacterial solution to streak on the MRS solid medium, and culture at 37°C for 24 hours to obtain a single colony.

[0048] (2) Preparation: After the ultra-clean workbench is sterilized, light the alcohol lamp, take out the sterilized 1.5ml centrifuge tube, and absorb 30μl of double distilled water (ddH 2 O), pick a single bacterium with a sterilized pipette near the flame and pipette and mix in double distilled water to form a bacterial suspension with a certain turbidity.

[0049] (3) Pretreatment: -20°C refrigerator (15min) - 65°C water bath (10min) repeated freezing and thawing three times, after flicking, centrifuge at 6000r / m for 5s in a hand-held centrifuge to make the liquid on the tube wall fall back.

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Abstract

The invention discloses lactobacillus plantarum and uses thereof, and particularly discloses lactobacillus plantarum and uses thereof in preparation of medicines or foods, the medicines, and the foods. The lactobacillus plantarum is deposited in the China Center for Type Culture Collection at Wuhan University in Wuhan, China, in September 6, 2013 with an accession number being CCTCC NO:M2013391 and an accession name being Lactobacillus plantarum Y-9. The lactobacillus plantarum has excellent probiotic functions, acid resistance and cholate resistance, can be stored for a long term, and can be used for effectively reducing cholesterol in blood, lowering blood pressure, boosting immunity, losing weight or adjusting intestinal flora.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular to plantaractobacillus and its use, more specifically to plantaractobacillus and its use in preparing medicine or food, medicine and food. Background technique [0002] With the continuous change of modern human eating habits, the intake of high-fat food accounts for a relatively high proportion in the entire dietary structure. Coupled with environmental pollution, high work and study pressures and other problems that have emerged in recent years, diseases such as hyperlipidemia, high blood pressure, decreased immunity, obesity, and imbalance of intestinal flora have seriously endangered human health. [0003] The sources of intestinal microorganisms can be divided into native bacteria, foreign bacteria and commensal flora. They form an interdependent and mutually restrictive unity with the host environment. The microbiota that permanently settles in the human intestinal trac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/06A61P9/12A61P37/04A61P3/04A61P1/00A23L1/29C12R1/25A23L33/00
CPCA61K35/747C12N1/205C12R2001/25
Inventor 宋垚刘峰顾锐陈富华仇婷刘力硕张兴举张耕耘
Owner 深圳华大基因农业控股有限公司
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