A kind of in vitro culture method of rat vascular endothelial cells

Abstract

The invention provides an in-vitro culturing method for vascular endothelial cells of a rat. The method comprises the following steps: a rat aorta is obtained, washed with a sterile PBS (phosphate buffered saline) solution and then soaked in a DMEM (Dulbecco's modified Eagle's medium)-F12 nutrient solution containing heparin; aortic intima is turned out with a sterile needle and thread, and knots are tied at two ends; the overturned aorta is digested in collagenase; digested aorta is placed in a serum-free DMEM-F12 nutrient solution after cultured in an incubator at the temperature of 37 DEG C, and is cut into small aortic slices; the aortic slices are subjected to adherent culture, the aortic intima parts of the aortic slices are soaked in a DMEM-F12 nutrient solution containing heparin and serum, and the nutrient solution is replaced every 48 h; the aortic slices are moved in the third-fifth days, later, the nutrient solution is replaced every day, a large quantity of vascular endothelial cells can be obtained on the ninth day, and the vascular endothelial cells can be used for passage after the fourteenth day.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for culturing rat vascular endothelial cells in vitro. Background technique [0002] Cardiovascular disease is a class of diseases that pose a great threat to human health. The formation of atherosclerotic lesions and angiogenesis after vascular injury both involve the activation of vascular endothelial cells. Vascular endothelial cells are ideal tool cells for vascular pathology research. Therefore, how to obtain purified vascular endothelial cells easily and stably has always been a challenge. popular research directions. At present, there are a large number of literature reports on the culture method of human umbilical vein endothelial cells and their wide application in the study of vascular pathology, but because human umbilical vein endothelial cells are derived from veins rather than arteries, their research value is limited; at the same time There ...

AI Technical Summary

Problems solved by technology

It is difficult to avoid the friction between cells during the flipping process, which increases the chance of cell damage and contamination

The diameter of the aorta of 160-220g rats is only about 2mm, and the thickness of the vessel wall itself, so the success rate of intimal inversion operation is low and the blood vessels are not fully utilized

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