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Sodium polyacrylate-grafted and sodium alginate-embedded probiotic microcapsule and preparation method thereof

A technology of sodium polyacrylate and sodium alginate, used in microcapsules, capsule delivery, pharmaceutical formulations, etc., can solve the problems of large gel pores and inability to effectively block the infiltration of gastric acid.

Inactive Publication Date: 2015-05-27
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the problem that the gel pores formed by the cross-linking of sodium alginate and calcium ions are too large and cannot effectively block the infiltration of gastric acid, the present invention starts with increasing the carboxyl content of sodium alginate, devotes itself to the transformation of the inner wall material structure, and provides a Sodium polyacrylate grafted with sodium alginate to embed probiotic microcapsules and preparation method thereof

Method used

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  • Sodium polyacrylate-grafted and sodium alginate-embedded probiotic microcapsule and preparation method thereof
  • Sodium polyacrylate-grafted and sodium alginate-embedded probiotic microcapsule and preparation method thereof
  • Sodium polyacrylate-grafted and sodium alginate-embedded probiotic microcapsule and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0034] Example 1: Synthesis and characterization of sodium polyacrylate grafted with sodium alginate 1# (Alg-PAAs1)

[0035] Dissolve 1 g of sodium alginate (Alg) (weight-average molecular weight: 2×106) in 20 mL of water, the concentration of Alg is about 50 mg / mL, stir well until it is completely dissolved, heat up to 58 °C under nitrogen atmosphere, and continue Stir for 30 min, add 0.09 g of ammonium persulfate, and initiate at this temperature for 15 min; neutralize the acrylic acid monomer with sodium hydroxide solution under ice-water bath conditions, and slowly drop 0.72 g of sodium acrylate monomer into the Alg solution , raised the temperature to 70°C for 2 h, adjusted the pH of the solution to 6.5, dialyzed the solution against water for 2 d, and freeze-dried to obtain sodium polyacrylate grafted sodium alginate 1# (Alg-PAAs1). figure 1As shown, the detection results show that the z / (x+y) in the Alg-PAAs1 structure is about 2:1, which is basically close to the feedi...

Embodiment 2

[0036] Example 2: Synthesis and characterization of sodium polyacrylate grafted with sodium alginate 2# (Alg-PAAs2)

[0037] Dissolve 1 g of sodium alginate (Alg) (weight-average molecular weight: 3×106) in 100 mL of water, the concentration of Alg is about 10 mg / mL, stir well until it is completely dissolved, heat up to 62 °C under nitrogen atmosphere, and continue Stir for 60 min, add 0.20 g of ammonium persulfate, and initiate at this temperature for 15 min; neutralize the acrylic acid monomer with sodium hydroxide solution under ice-water bath conditions, and slowly drop 2.88 g of sodium acrylate monomer into the Alg solution , raised the temperature to 80°C for 3 h, adjusted the pH to 7.4, dialyzed the solution against water for 3 d, and freeze-dried to obtain sodium polyacrylate grafted sodium alginate 2# (Alg-PAAs2). z / (x+y) is approximately 8:1. Calculation based on the feed ratio: x / (x+y)=10:50 in the structural formula of Alg-PAAs1.

[0038] Material Viscosity ...

Embodiment 3

[0043] Example 3: Preparation and Characterization of Hollow Microcapsules

[0044] 1. Preparation of Alg / Alg-PAAs1 microcapsules

[0045] Mix 1 wt% Alg-PAAs1 and 3 wt% Alg solution at a mass ratio of 1:1, add dropwise to 0.3 mol / L calcium chloride solution, and transfer to 0.5 wt% pH after solidification at 4°C for 60 min =5.8 chitosan solution for 15 min, washed with sterile water to obtain the hollow microstructure with sodium alginate and sodium polyacrylate grafted with sodium alginate 1# (Alg / Alg-PAAs1) as the inner wall material capsule.

[0046] 2. The 2 wt% Alg solution was made into hollow microcapsules with sodium alginate (Alg) as the inner wall material by the same method.

[0047] Characterization of Alg / Alg-PAAs1 Hollow Microcapsules

[0048] 1) Particle size of microcapsules

[0049] Blot the residual water on the surface of Alg / Alg-PAAs1 or Alg hollow microcapsules on filter paper, and measure the particle diameters of 10 microspheres randomly with a verni...

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Abstract

The invention discloses a sodium polyacrylate-grafted and sodium alginate-embedded probiotic microcapsule, which is prepared by carrying out ionic crosslinking and embedding on probiotic with a complex of sodium polyacrylate-grafted sodium alginate and sodium alginate as an inner layer wall material, and coating chitosan on an outer layer. The embedded probiotic is one or more than two of lactobacillus, galactococcus or bifidobacterium; and the sodium polyacrylate is a state-approved food additive, and is safe and nontoxic in embedding. The buffer action on gastric acid caused by the inner layer wall material is increased through a grafting method; reduction of viable count in a gastric juice environment is reduced; meanwhile, the mechanical strength of the microcapsule is enhanced by cross-linking property of the capsule and calcium ions; the viscosity of a wall material solution is lower than that of an existing high polymer material; the preparation difficulty of the microcapsule is reduced; the prepared microcapsule has the functions of resisting gastric juice and accelerating intestinal release; the effective living bacteria count of intestinal tracts can be improved when the microcapsule is applied to embedding the probiotic; and the in-vivo probiotic function is enhanced.

Description

technical field [0001] The invention belongs to the technical field of preparation of probiotic products, in particular to sodium polyacrylate grafted with sodium alginate to embed probiotic microcapsules and a preparation method thereof. Background technique [0002] In today's society, with the increasing pressure of people's life and work, coupled with the influence of environmental pollution and other reasons, the sub-health population continues to increase. Drinking beverages containing probiotics has become a good choice for people's health care. Studies have confirmed that probiotics have the functions of maintaining the ecological balance of the intestinal tract, preventing cancer and improving the body's immunity. The health effects of probiotics are closely related to the number of live bacteria reaching the intestinal tract. However, gastric acid, bile salts, and digestive enzymes in the human body all have bactericidal effects (the bactericidal effect of gastri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K35/744A61K35/745A61K35/747A61K47/36A61P1/00A61P37/04A61P35/00
Inventor 王艳萍刘媛孙也
Owner TIANJIN UNIV OF SCI & TECH
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