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Bacteriocin bacteriostatic fresh-keeping biological composite film

A biocomposite film and bacteriocin technology, applied in the field of fresh-keeping, can solve problems such as poor antibacterial activity and mechanical properties, achieve good fresh-keeping effect, low production cost, and promote adsorption

Active Publication Date: 2015-05-20
安徽民祯生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polylactic acid has been used as a packaging material due to its degradability and plasticity. If bacteriocin is directly added to a film made of polylactic acid, the hydrophobic properties of polylactic acid will limit the combination of bacteriocin and bacteriocin in hydrophobic polymers. Incompatibility causes phase separation in the membrane, resulting in poor antimicrobial activity and mechanical properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this embodiment, a method for manufacturing a bacteriocin antibacterial and fresh-keeping biocomposite film includes pretreatment of sawdust, film making, dry heat treatment of the biocomposite film and diffusion coating of bacteriocin. The specific operation steps are as follows:

[0027] 1) Pretreatment of sawdust particle raw material for biocomposite film components: use a mill to make sawdust into 100-300μm particles, then dry in a hot air circulation oven at 70°C for 24 hours; then place the dried sawdust in absolute ethanol Stir at room temperature for 1 hour to remove impurities on the surface; dry the treated sawdust at 70°C for 3 hours to evaporate the solvent, then soak in 10% sodium hydroxide solution for 3 hours at 40°C to remove natural impurities including pectin, Lignin, waxy substances and natural oils; then rinsed with distilled water to remove sodium hydroxide, and dried at 60°C for 24h.

[0028] 2) Film making: 5% (w / w) of the above-mentioned pre...

Embodiment 2

[0033] 1) The sawdust is made into 100-300 μm particles with a mill, and then dried in a hot air circulation oven at 70°C for 24 hours; then the dried sawdust is placed in absolute ethanol and stirred at room temperature for 1 hour; the processed sawdust is Dry at 70°C for 3h to evaporate the solvent, then soak in 10% sodium hydroxide solution at 40°C for 3h; then rinse with distilled water to remove sodium hydroxide, and dry at 60°C for 24h.

[0034] 2) Stir and mix 3% (w / w) of the above-mentioned pretreated sawdust particles with 97% (w / w) polylactic acid, add them into a twin-screw extruder, and then blow them into a film with a thickness of 400- 500 μm.

[0035]3) The film sample is placed in a hot air circulation oven at 90°C for 2 hours;

[0036] 4) Soak the film in 0.2% (w / v) nisin solution for 30min at room temperature. Then dry it under natural air flow for 30-60 minutes, seal the package, and store it in a refrigerator at about 5°C.

[0037] Using this plastic wra...

Embodiment 3

[0039] 1) The sawdust is made into 100-300 μm particles with a mill, and then dried in a hot air circulation oven at 70°C for 24 hours; then the dried sawdust is placed in absolute ethanol and stirred at room temperature for 1 hour; the processed sawdust is Dry at 70°C for 3h to evaporate the solvent, then soak in 10% sodium hydroxide solution at 40°C for 3h; then rinse with distilled water to remove sodium hydroxide, and dry at 60°C for 24h.

[0040] 2) 6% (w / w) of the above-mentioned pretreated sawdust particles and 94% (w / w) polylactic acid are mixed evenly, added to the twin-screw extruder, and then blown into a film with a film thickness of 400- 500 μm.

[0041] 3) The film sample is placed in a hot air circulation oven at 90°C for 2 hours;

[0042] 4) Soak the film in 0.2% (w / v) pediocin lactate solution for 30min at room temperature. Then dry it under natural air flow for 30-60 minutes, seal the package, and store it in a refrigerator at about 5°C.

[0043] Using thi...

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Abstract

The invention discloses a bacteriocin bacteriostatic fresh-keeping biological composite film. The biological composite film is prepared by preparing a film matrix from 94-97wt% of polylactic acid and 3-6wt% of sawdust particles and adsorbing a trace amount of bacteriocin on the film surface. A manufacturing method comprises the following steps of stirring and mixing the pretreated sawdust particles and polylactic acid, extruding and blowing the film; carrying out dry heat treatment on the biological composite film and implanting the bacteriocin into the film by virtue of a diffusion coating method. The manufacturing method is simple and low in production cost, the bacteriocin can be effectively adsorbed on the surface of the biological composite film and the industrial production can be realized. The prepared bacteriocin bacteriostatic fresh-keeping biological composite film has the advantages of stronger effects on inhibiting activities of multiple food-borne pathogenic bacteria and food spoilage bacteria, very low solubility in water and complete biodegradability and is especially suitable for fresh-keeping preservation of fresh meat and other high-moisture foods.

Description

technical field [0001] The invention belongs to the field of fresh-keeping technology, and in particular relates to a bacteriocin antibacterial and fresh-keeping biocomposite film. Background technique [0002] Fresh-keeping film has been widely used in people's life. In addition to traditional moisturizing and air-conditioning fresh-keeping film products, some fresh-keeping products made of antibacterial and antibacterial materials have gradually appeared. Some add some chemical antibacterial ingredients such as ethyl p-hydroxybenzoate, sorbic acid, propylene glycol, etc. to the food preservation film material to make the food preservation film obtain the antibacterial effect, but chemical preservatives will bring health hazards and environmental pollution And other issues. Some are added with natural ore powders that can generate negative ions, such as ferrite and magnesium tourmaline, but less negative ions are produced, the preservation time is shorter, and the film thi...

Claims

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Application Information

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IPC IPC(8): C08L67/04C08L97/02
Inventor 宋建民张琴王德海宛荣生彭慕华
Owner 安徽民祯生物工程有限公司
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