Cardiac troponin I rapid detection method and corresponding detection kit

Abstract

The invention relates to a cardiac troponin I rapid detection method. The cardiac troponin I rapid detection method is characterized by comprising the following steps: step (1): mixing nanoscale light sensitive microspheres with dyes inside, luminous microspheres coated by active molecules, active molecules labeled by biotins and a sample to be tested to obtain a mixture; step (2): carrying out incubation culture on the mixture, enabling components having immunological characteristics to react to obtain a compound; and step (3): irradiating the compound with exciting light to enable the compound to emit light, and determining by using a photon counter to obtain the detection value. The invention also provides a cardiac troponin I rapid detection kit. According to the cardiac troponin I rapid detection method and the corresponding detection kit, the detection result is stable, the accuracy is high, the cost is low, the sensitivity is high, the precision is high, the detection range is wide and the method and the kit are suitable for clinical expansion.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to immunoassay, specifically a rapid detection method for cardiac troponin I and a corresponding detection kit. Background technique [0002] Human cardiac troponin I (cTnI) is a cardiac protein with a molecular weight of 22.5KD. It forms a cardiac troponin complex with troponin T (TnT) and troponin C (TnC) to complete intracellular movement together. Fundamental functions of calcium signaling in protein-protein interactions. Cardiac troponin I (cTnI) is expressed in myocardial tissue, but not in fetal, healthy or adult skeletal muscle in a diseased state, so it is highly specific to the myocardium. Because troponin has the advantages of strong tissue specificity, long diagnostic window period, rapid determination method, and early appearance in blood, its role in the diagnosis of acute myocardial infarction (AMI) has attracted more and more attention. cTn is considered ...

AI Technical Summary

Problems solved by technology

However, due to the relatively backward detection method of cTnI in the past and high false positives, it has affected its clinical value. In recent years, due to the update of detection technology, a fast, simple and reliable method for the determination of cTnI has been established rapidly.

[0018] Colloidal gold and chemiluminescence are common detection methods for cTnI in the market. Although colloidal gold is low in cost, it can only be semi-quantitative or qualitative. There are limitations such as low detection sensitivity and poor precision. Chemiluminescence can be quantitative but the cost is high.

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