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Method for inhibiting dysosma versipellis explant from contamination and browning

A technology for explants and star anise lotus, which is applied in the field of inhibiting explant pollution and browning, can solve the problems of explant death, easy browning, bacterial contamination, etc. low effect

Inactive Publication Date: 2015-05-06
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are few research reports on the tissue culture and rapid propagation technology of star anise lotus, and its main problem is that serious bacterial pollution is easily produced in the tissue culture process of star anise lotus, and the pollution rate reaches 100%; meanwhile, because star anise lotus contains a large amount of phenolic substances , it is very easy to produce browning phenomenon; the cut stem and leaf tissue release brown substances from the surface to the medium, at this time the medium gradually turns brown, and the explants also gradually turn brown and die, unable to survive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for suppressing pollution and browning of star anise lotus explants, comprising the following steps:

[0034] (1) select the wild star anise rhizome more than 5 years old as the mother plant, and transplant the mother plant to a river sand substrate with a humidity of 65% for pre-cultivation for 1 month until new stems and leaves grow;

[0035] (2) Take steps (1) Stems or young leaves that grow robustly and are free from diseases and insect pests are first placed in N-long chain thiocarboxylate betaine and soaked for 5 minutes, and then rinsed with distilled water for 1 hour. During the rinse process, use Soft hair brush scrubs the dirt on the surface of the explant;

[0036] (3) Place the explants treated in step (2) on the TiO-coated 2 and the nanoscale powder of piezoelectric crystal tourmaline in the tank body of neutral coating made of piezoelectric crystal tourmaline for 10 minutes, and in the tank body, pass into the mixed gas with nitrogen and ozone ma...

Embodiment 2

[0041] A method for suppressing pollution and browning of star anise lotus explants, comprising the following steps:

[0042] (1) select the rhizome of the wild star anise lotus that is more than 5 years old as the mother plant, and transplant the mother plant to a river sand substrate with a humidity of 85% for pre-cultivation for 1 month until new stems and leaves grow;

[0043] (2) Take steps (1) Stems or young leaves that grow robustly and are free from diseases and insect pests are first placed in N-long chain thiocarboxylate betaine and soaked for 5 minutes, and then rinsed with distilled water for 1 hour. During the rinse process, use Soft hair brush scrubs the dirt on the surface of the explant;

[0044] (3) Place the explants treated in step (2) on the TiO-coated 2 and piezoelectric crystal tourmaline nano-scale powder made of neutral coating tank body for 15min, and in the tank, pass into the mixed gas with nitrogen and ozone mass ratio of 10:1, and then use concent...

Embodiment 3

[0049] A method for suppressing pollution and browning of star anise lotus explants, comprising the following steps:

[0050] (1) Select the rhizome of wild star anise lotus more than 5 years old as the mother plant, and transplant the mother plant to a river sand substrate with a humidity of 75% for pre-cultivation for 1 month until new stems and leaves grow;

[0051] (2) Take steps (1) Stems or young leaves that grow robustly and are free from diseases and insect pests are first placed in N-long chain thiocarboxylate betaine and soaked for 5 minutes, and then rinsed with distilled water for 1 hour. During the rinse process, use Soft hair brush scrubs the dirt on the surface of the explant;

[0052] (3) Place the explants treated in step (2) on the TiO-coated 2 and piezoelectric crystal tourmaline nano-scale powder made of neutral coating tank body for 12min, and in the tank body into nitrogen and ozone mass ratio of 10:1 mixed gas, and then with a concentration of 2.5% Ste...

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Abstract

The invention discloses a method for inhibiting a dysosma versipellis explant from contamination and browning. The method comprises the following steps: transplanting a wild dysosma versipellis rhizome which is more than five-year old into clean river sand matrix for preculture; soaking a stem growing strongly into N-long-chain thiocarboxylic acid glycine betaine, washing with distilled water, and brushing the dirt on the surface of the explant; treating the explant by use of anion photocatalysis, sterilizing with sodium hypochlorite, washing with sterile water, sucking moisture on the surface by use of aseptic filter paper until the surface is dry, cutting into squares, and soaking in a heavy metal complexing agent; inoculating onto a modified MS medium for dark culture for two weeks; and then performing bright culture. The method can be used of effectively preventing the dysosma versipellis explant from contamination and browning and providing sufficient aseptic explants for the tissue culture research of the medicinal plant-dysosma versipellis, so that the follow-up tissue culture success rate is ensured, the cost is low and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for inhibiting pollution and browning of explants, in particular to a tissue culture method for inhibiting pollution and browning of star anise lotus explants. Background technique [0002] The medicinal plant star anise belongs to the perennial herb of the genus Podophyllum in the family Berberidaceae, and is one of the traditional main plant sources of podophyllotoxin, the synthetic precursor of anticancer drugs such as etoposide and teniposide. Due to over-excavation, habitat destruction and slow plant growth, the wild resources of star anise lotus are almost exhausted, and the species is endangered. It has been listed as a national rare and endangered third-level protected plant. Therefore, by adopting medicinal plant tissue culture propagation technology to obtain the callus or sterile seedlings of I. chinensis, the resource pressure of I. chinensis can be alleviated, and it can also provide usable aseptic materia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 谭小明周雅琴余丽莹韦莹
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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