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A Simple Method for Extracting and Determining Peanut Fatty Acids

A fatty acid and peanut technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of harsh operating conditions and short service life

Active Publication Date: 2018-12-14
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, diazomethane is an efficient and fast transesterification catalyst, which can also be used for the esterification of free fatty acids and phosphate esters, but it is not widely used due to harsh operating conditions and short service life

Method used

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  • A Simple Method for Extracting and Determining Peanut Fatty Acids
  • A Simple Method for Extracting and Determining Peanut Fatty Acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Samples were taken at different stages of the seed development of the peanut variety Huayu 19, starting from 25 days after the needles went to the ground (DAP), and samples were taken every 7 days, and a total of 6 samples were taken. Peel off the husk, keep the seeds, grind repeatedly with liquid nitrogen, and then freeze-dry the ground seeds to obtain dry tissues. Use a universal balance to accurately weigh 0.01g (m) of dried peanut tissue, put it into a 5ml crimp headspace bottle that has been cleaned and dried by alcohol, add 850μl of methanol and 100μl of hydrochloric acid, and ultrasonically (300w, 28kHz) for 10min Mix well. Then 50 μl of 2,2-dimethoxypropane was added to the bottle. Use a capper to seal the headspace vial with an aluminum cap with a polytetrafluoroethylene pad, and react in a water bath at 85°C for 1 h. Oscillate once every 10 minutes during the methyl esterification process. After the reaction, take it out and cool to room temperature. After...

Embodiment 2

[0023] The peanut variety Huayu 19 was grown in a 16-hour light / 8-hour dark (28°C / 22°C) light incubator. Three kinds of peanut tissues including roots, stems and leaves were taken from three-leaf seedlings that grew two weeks after germination, and flowers were taken from peanut plants that grew about 60 days after germination. The samples were washed 3 times with sterilized distilled water, and dried tissues were obtained after freeze-drying. Accurately weigh 0.01 g (m) of dry peanut tissue with a universal balance, put it into a mortar, add liquid nitrogen and grind repeatedly to destroy the cell wall of the tissue. Add 850 μl of methanol to the mortar and transfer the tissue extract and residue thoroughly to a 5 ml crimp top headspace vial. Then add 100 μl of hydrochloric acid to the bottle, and mix with ultrasound (300w, 28kHz) for 10min. Then 50 μl of 2,2-dimethoxypropane was added to the bottle, and the headspace bottle was sealed with an aluminum cap with a polytetraf...

Embodiment 3

[0027] Prepare the internal standard stock solution first. Weigh 10 mg of internal standard nonadecanoic acid (purchased from Aladdin), accurately weigh it, place it in a 10 ml volumetric flask, add isoamyl alcohol to the mark, shake well, transfer it to a sealed screw-top test tube, and store it in cold storage. Obtain internal standard stock solution. Use a universal balance to weigh 0.01g (m) of mature air-dried seeds of wild-type Arabidopsis (Col-0), put them into a 5ml crimp-top headspace bottle that has been cleaned and dried by alcohol, and use a clean glass rod that has been cleaned. Crush the seeds. Add 850 μl of methanol and 100 μl of hydrochloric acid to the bottle, sonicate (300w, 28kHz) for 10 minutes to mix well, and rinse the glass rod. Then 50 μl of 2,2-dimethoxypropane was added to the bottle, and then 36 μl of nonadecanoic acid internal standard stock solution with a concentration of 1 mg / ml was added. Use a capper to seal the headspace vial with an alumin...

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Abstract

The invention discloses a simple method for extracting and determining peanut fatty acids, which comprises the following steps: carrying out freeze-drying on a fresh sample to obtain dry tissues; weighing the dry sample, putting into a mortar, and grinding and pulverizing with liquid nitrogen; or putting the dry sample into a jaw-top empty bottle, and crushing with a glass bar; adding reagents I and II, evenly mixing by using ultrasonic, adding a reagent III, optionally adding an internal standard, and carrying out water bath reaction; after the reaction is finished, cooling, adding an organic reagent and a salt solution, evenly mixing, sucking out the solution, transferring into a centrifuge tube, and standing; carrying out centrifugal separation, and absorbing the supernate for machine determination; and calculating the content of the fatty acid component by a normalization process or internal standard process. The method simplifies the sample pretreatment process, is simple and quick to operate, greatly shortens the testing time, has the advantages of low cost, low quantity of required samples and favorable universality, and is a simple and accurate method for extracting and measuring peanut fatty acids in the laboratory.

Description

technical field [0001] The invention relates to a simple method for extracting and measuring peanut fatty acid, belonging to the technical field of fatty acid extraction. Background technique [0002] Peanut is an important oil crop, cash crop and foreign exchange earning crop in China. The quality of peanut kernel oil is determined by the composition of fatty acids. Peanut fatty acid is mainly composed of eight components including palmitic acid, stearic acid, oleic acid, linoleic acid, arachidic acid, arachidic acid, behenic acid and tetracosanoic acid, among which oleic acid and linoleic acid are the main components. , accounts for about 80%, and is necessary for human health. For example, oleic acid has the function of reducing harmful cholesterol and maintaining the level of beneficial cholesterol in the lipid metabolism of the human body, thereby slowing down atherosclerosis and effectively preventing cardiovascular and cerebrovascular diseases such as coronary heart ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 迟晓元孙全喜潘丽娟陈娜陈明娜王通王冕禹山林杨珍
Owner SHANDONG PEANUT RES INST
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