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Gas emission determinator and method for determining trace nitrogen emission flux using same

A gas emission and measurement device technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as low measurement results, inability to distribute acetylene evenly, and inability to completely inhibit microbial reduction, and achieve the effect of eliminating nitrogen leakage

Active Publication Date: 2015-03-25
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the fact that acetylene cannot be evenly distributed in the soil in real life, and some denitrifying microorganisms have certain resistance to acetylene, they cannot completely inhibit the reduction of microorganisms, which eventually leads to seriously low measurement results.

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  • Gas emission determinator and method for determining trace nitrogen emission flux using same

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specific Embodiment 1

[0029] Specific embodiment 1, when the culture starts, the nitrogen concentration C in the inner sealed tank 3 2 50μg L -1 , Nitrogen concentration C in inner sealed tank 3 after cultivation 1 52.14μg L -1 , The gas volume V of the inner sealed tank 3 is 1.14L, and the soil column area A is 0.038m 2 , When the incubation time T is 10h, the method of using the gas emission measuring device to measure the trace nitrogen emission flux includes the following steps:

[0030] A. Prepare the undisturbed soil and place the soil to be measured into undisturbed soil pillars 5 and place them in the inner sealed tank 3. The height of the undisturbed soil pillars is 130mm. According to the height of the soil pillars, determine the nitrogen emission flux F N In the calculation formula, the gas volume V of the inner sealed tank 3 is 1.14L, and the inner rubber sealing cover 1 made of butyl rubber is used to seal the inner sealed tank 3, because the butyl rubber has low air permeability and good ai...

specific Embodiment 2

[0036] Specific embodiment 2, when the cultivation starts, the nitrogen concentration C in the inner sealed tank 3 2 100μg L -1 , Nitrogen concentration C in inner sealed tank 3 after cultivation 1 150.3μg L -1 , The gas volume V of the inner sealed tank 3 is 1.90L, and the soil column area A is 0.038m 2 , When the incubation time T is 5h, the method of using the gas emission measuring device to measure the trace nitrogen emission flux includes the following steps:

[0037] A. Prepare the undisturbed soil and place the soil to be measured into undisturbed soil pillars 5 and place them in the inner sealed tank 3. The height of the undisturbed soil pillars is 110mm. According to the height of the soil pillars, determine the nitrogen emission flux F N In the calculation formula, the gas volume V of the inner sealed tank 3 is 1.14L, and the inner rubber sealing cover 1 made of butyl rubber is used to seal the inner sealed tank 3, because the butyl rubber has low air permeability and goo...

specific Embodiment 3

[0043] Specific embodiment 3, when the culture starts, the nitrogen concentration C in the inner sealed tank 3 2 100μg L -1 , Nitrogen concentration C in inner sealed tank 3 after cultivation 1 133.3μg L -1 , The gas volume of the inner sealed tank 3 is 2.28L, and the soil column area A is 0.038m 2 , When the incubation time T is 2h, the method of using the gas emission measuring device to measure the trace nitrogen emission flux includes the following steps:

[0044] A. Prepare the undisturbed soil and place the soil to be measured into undisturbed soil pillars 5 and place them in the inner sealed tank 3. The height of the undisturbed soil pillars is 100mm. According to the height of the soil pillars, the nitrogen emission flux F N In the calculation formula, the gas volume V of the inner sealed tank 3 is 2.28L, and the inner rubber sealing cover 1 made of butyl rubber is used to seal the inner sealed tank 3, because the butyl rubber has low air permeability and good air tightness....

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Abstract

The invention discloses a gas emission determinator and a method for determining the trace nitrogen emission flux using the same. The gas emission determinator comprises an inner layer seal tank capable of being sealed, and an outer layer seal tank capable of being sealed, wherein the inner layer seal tank is arranged in the outer layer seal tank in a sleeving manner, and meanwhile is filled with an undisturbed soil column, and an inert gas buffer layer is arranged between the inner layer seal tank and the undisturbed soil column. The method for determining the trace nitrogen emission flux using the gas emission determinator comprises the following implementation steps: A, preparing undisturbed oil; B, replacing gas in a clearance between the inner layer seal tank and the undisturbed soil column; C, replacing gas between the inner layer seal tank and the outer layer seal tank; D, culturing; and E, determining the content of nitrogen in the inner layer seal tank, and calculating the undisturbed soil column nitrogen emission flux. The gas emission determinator and the method for determining the trace nitrogen emission flux using the same have the beneficial effects that the inert gas buffer layer positioned between the inner layer seal tank and the outer layer seal tank can be used for obviously reducing and even eliminating the nitrogen leakage, so that the trace nitrogen flux with high background concentration can be determined accurately.

Description

Technical field [0001] The invention provides a gas emission measuring device and a method for measuring the emission flux of trace nitrogen using the same. Background technique [0002] In ecological research, it is often necessary to measure the trace nitrogen emission flux of the ecosystem under high background concentration conditions. The current trace nitrogen emission flux under high background concentration conditions is generally measured by the acetylene suppression method, that is, the trace nitrogen emission flux is indirectly calculated from the measurement of nitrous oxide by inhibiting the microbial process of reducing nitrous oxide to nitrogen. However, in real life, acetylene cannot be evenly distributed in the soil, and at the same time, some denitrifying microorganisms have certain resistance to acetylene, which cannot completely inhibit the reduction of microorganisms, which ultimately leads to a serious low test result. Summary of the invention [0003] The p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/00
Inventor 秦树平胡春胜张玉铭董文旭
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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